Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1960 and GFF1961 are separated by 372 nucleotides GFF1961 and GFF1962 are separated by 35 nucleotides GFF1962 and GFF1963 overlap by 4 nucleotides GFF1963 and GFF1964 are separated by 26 nucleotides GFF1964 and GFF1965 are separated by 28 nucleotides
GFF1960 - Cell wall hydrolyses involved in spore germination, at 1,986,479 to 1,987,501
GFF1960
GFF1961 - Entericidin A/B family protein, at 1,987,874 to 1,988,002
GFF1961
GFF1962 - Twin-arginine translocation protein TatC, at 1,988,038 to 1,988,829
GFF1962
GFF1963 - Twin-arginine translocation protein TatB, at 1,988,826 to 1,989,281
GFF1963
GFF1964 - Twin-arginine translocation protein TatA, at 1,989,308 to 1,989,547
GFF1964
GFF1965 - Segregation and condensation protein B, at 1,989,576 to 1,990,169
GFF1965
Position (kb)
1988
1989 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1987.039 kb on - strand, within GFF1960 at 1987.048 kb on + strand, within GFF1960 at 1987.048 kb on + strand, within GFF1960 at 1987.056 kb on - strand, within GFF1960 at 1987.056 kb on - strand, within GFF1960 at 1987.056 kb on - strand, within GFF1960 at 1987.061 kb on + strand, within GFF1960 at 1987.069 kb on - strand, within GFF1960 at 1987.078 kb on + strand, within GFF1960 at 1987.080 kb on + strand, within GFF1960 at 1987.080 kb on + strand, within GFF1960 at 1987.108 kb on + strand, within GFF1960 at 1987.109 kb on + strand, within GFF1960 at 1987.109 kb on + strand, within GFF1960 at 1987.109 kb on + strand, within GFF1960 at 1987.110 kb on - strand, within GFF1960 at 1987.117 kb on - strand, within GFF1960 at 1987.118 kb on - strand, within GFF1960 at 1987.120 kb on + strand, within GFF1960 at 1987.120 kb on + strand, within GFF1960 at 1987.140 kb on + strand, within GFF1960 at 1987.142 kb on - strand, within GFF1960 at 1987.183 kb on + strand, within GFF1960 at 1987.208 kb on - strand, within GFF1960 at 1987.223 kb on + strand, within GFF1960 at 1987.234 kb on - strand, within GFF1960 at 1987.241 kb on + strand, within GFF1960 at 1987.258 kb on + strand, within GFF1960 at 1987.295 kb on + strand, within GFF1960 at 1987.295 kb on - strand, within GFF1960 at 1987.300 kb on + strand, within GFF1960 at 1987.305 kb on + strand, within GFF1960 at 1987.313 kb on - strand, within GFF1960 at 1987.326 kb on - strand, within GFF1960 at 1987.331 kb on + strand, within GFF1960 at 1987.331 kb on + strand, within GFF1960 at 1987.331 kb on + strand, within GFF1960 at 1987.331 kb on + strand, within GFF1960 at 1987.336 kb on + strand, within GFF1960 at 1987.339 kb on - strand, within GFF1960 at 1987.339 kb on - strand, within GFF1960 at 1987.344 kb on - strand, within GFF1960 at 1987.349 kb on - strand, within GFF1960 at 1987.362 kb on + strand, within GFF1960 at 1987.377 kb on + strand, within GFF1960 at 1987.385 kb on - strand, within GFF1960 at 1987.387 kb on - strand, within GFF1960 at 1987.395 kb on - strand, within GFF1960 at 1987.395 kb on - strand, within GFF1960 at 1987.399 kb on + strand at 1987.407 kb on - strand at 1987.407 kb on - strand at 1987.472 kb on + strand at 1987.472 kb on - strand at 1987.495 kb on + strand at 1987.503 kb on - strand at 1987.503 kb on - strand at 1987.523 kb on + strand at 1987.602 kb on + strand at 1987.629 kb on - strand at 1987.670 kb on - strand at 1987.698 kb on + strand at 1987.706 kb on - strand at 1987.726 kb on - strand at 1987.755 kb on + strand at 1987.755 kb on + strand at 1987.786 kb on + strand at 1987.792 kb on - strand at 1987.805 kb on + strand at 1987.813 kb on - strand at 1987.821 kb on - strand at 1987.825 kb on + strand at 1987.835 kb on - strand at 1987.881 kb on + strand at 1987.889 kb on - strand, within GFF1961 at 1987.906 kb on + strand, within GFF1961 at 1987.918 kb on + strand, within GFF1961 at 1987.948 kb on - strand, within GFF1961 at 1987.952 kb on + strand, within GFF1961 at 1987.955 kb on - strand, within GFF1961 at 1987.958 kb on + strand, within GFF1961 at 1987.966 kb on - strand, within GFF1961 at 1987.966 kb on - strand, within GFF1961 at 1987.966 kb on - strand, within GFF1961 at 1987.971 kb on - strand, within GFF1961 at 1987.971 kb on - strand, within GFF1961 at 1987.973 kb on + strand, within GFF1961 at 1988.009 kb on - strand at 1988.014 kb on - strand at 1988.854 kb on - strand at 1988.909 kb on - strand, within GFF1963 at 1988.916 kb on - strand, within GFF1963 at 1988.961 kb on - strand, within GFF1963 at 1989.028 kb on - strand, within GFF1963 at 1989.296 kb on - strand at 1989.306 kb on - strand at 1989.314 kb on - strand at 1989.567 kb on - strand at 1989.582 kb on - strand at 1989.615 kb on + strand at 1989.623 kb on - strand at 1989.623 kb on - strand at 1989.648 kb on + strand, within GFF1965 at 1989.661 kb on - strand, within GFF1965 at 1989.682 kb on + strand, within GFF1965 at 1989.695 kb on - strand, within GFF1965 at 1989.723 kb on + strand, within GFF1965 at 1989.730 kb on - strand, within GFF1965 at 1989.744 kb on - strand, within GFF1965 at 1989.753 kb on + strand, within GFF1965 at 1989.753 kb on + strand, within GFF1965 at 1989.781 kb on - strand, within GFF1965 at 1989.787 kb on + strand, within GFF1965 at 1989.791 kb on - strand, within GFF1965 at 1989.801 kb on - strand, within GFF1965 at 1989.806 kb on + strand, within GFF1965 at 1989.811 kb on + strand, within GFF1965 at 1989.814 kb on - strand, within GFF1965 at 1989.819 kb on - strand, within GFF1965 at 1989.825 kb on - strand, within GFF1965 at 1989.825 kb on - strand, within GFF1965 at 1989.825 kb on - strand, within GFF1965 at 1989.827 kb on + strand, within GFF1965 at 1989.827 kb on + strand, within GFF1965
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,987,039 - GFF1960 0.55 +0.3 1,987,048 + GFF1960 0.56 -1.3 1,987,048 + GFF1960 0.56 -1.2 1,987,056 - GFF1960 0.56 +0.1 1,987,056 - GFF1960 0.56 -0.3 1,987,056 - GFF1960 0.56 +0.7 1,987,061 + GFF1960 0.57 -0.1 1,987,069 - GFF1960 0.58 -1.7 1,987,078 + GFF1960 0.59 -2.6 1,987,080 + GFF1960 0.59 -0.2 1,987,080 + GFF1960 0.59 -0.6 1,987,108 + GFF1960 0.61 -0.7 1,987,109 + GFF1960 0.62 -0.9 1,987,109 + GFF1960 0.62 -0.7 1,987,109 + GFF1960 0.62 +0.7 1,987,110 - GFF1960 0.62 -0.8 1,987,117 - GFF1960 0.62 -1.5 1,987,118 - GFF1960 0.62 -1.0 1,987,120 + GFF1960 0.63 +0.2 1,987,120 + GFF1960 0.63 -0.4 1,987,140 + GFF1960 0.65 +0.8 1,987,142 - GFF1960 0.65 -0.4 1,987,183 + GFF1960 0.69 -0.4 1,987,208 - GFF1960 0.71 +0.3 1,987,223 + GFF1960 0.73 -0.5 1,987,234 - GFF1960 0.74 +0.1 1,987,241 + GFF1960 0.74 -0.8 1,987,258 + GFF1960 0.76 -2.3 1,987,295 + GFF1960 0.80 -0.1 1,987,295 - GFF1960 0.80 -0.2 1,987,300 + GFF1960 0.80 +1.9 1,987,305 + GFF1960 0.81 +0.8 1,987,313 - GFF1960 0.82 -0.2 1,987,326 - GFF1960 0.83 +0.5 1,987,331 + GFF1960 0.83 -0.1 1,987,331 + GFF1960 0.83 -1.4 1,987,331 + GFF1960 0.83 +1.2 1,987,331 + GFF1960 0.83 -0.7 1,987,336 + GFF1960 0.84 +0.8 1,987,339 - GFF1960 0.84 -0.0 1,987,339 - GFF1960 0.84 -0.6 1,987,344 - GFF1960 0.85 -1.0 1,987,349 - GFF1960 0.85 -0.5 1,987,362 + GFF1960 0.86 -0.2 1,987,377 + GFF1960 0.88 -1.0 1,987,385 - GFF1960 0.89 -1.5 1,987,387 - GFF1960 0.89 +1.2 1,987,395 - GFF1960 0.90 -1.4 1,987,395 - GFF1960 0.90 -0.2 1,987,399 + -0.1 1,987,407 - -1.3 1,987,407 - +0.5 1,987,472 + +0.7 1,987,472 - -0.7 1,987,495 + +0.3 1,987,503 - -1.3 1,987,503 - +0.6 1,987,523 + -2.0 1,987,602 + -1.1 1,987,629 - -0.7 1,987,670 - -0.3 1,987,698 + +0.3 1,987,706 - -2.2 1,987,726 - -1.9 1,987,755 + -1.3 1,987,755 + -0.6 1,987,786 + -0.0 1,987,792 - +0.6 1,987,805 + -0.3 1,987,813 - -0.3 1,987,821 - -1.9 1,987,825 + -0.3 1,987,835 - -0.5 1,987,881 + +0.3 1,987,889 - GFF1961 0.12 -1.2 1,987,906 + GFF1961 0.25 -0.7 1,987,918 + GFF1961 0.34 -0.2 1,987,948 - GFF1961 0.57 -0.7 1,987,952 + GFF1961 0.60 +0.8 1,987,955 - GFF1961 0.63 +0.3 1,987,958 + GFF1961 0.65 +0.2 1,987,966 - GFF1961 0.71 -0.2 1,987,966 - GFF1961 0.71 +0.5 1,987,966 - GFF1961 0.71 -1.1 1,987,971 - GFF1961 0.75 -0.4 1,987,971 - GFF1961 0.75 -1.2 1,987,973 + GFF1961 0.77 -0.3 1,988,009 - +0.1 1,988,014 - +0.7 1,988,854 - +0.2 1,988,909 - GFF1963 0.18 +0.1 1,988,916 - GFF1963 0.20 +0.9 1,988,961 - GFF1963 0.30 -0.9 1,989,028 - GFF1963 0.44 -2.6 1,989,296 - -0.8 1,989,306 - +0.1 1,989,314 - -2.3 1,989,567 - -0.8 1,989,582 - +0.0 1,989,615 + +0.4 1,989,623 - +0.3 1,989,623 - +0.2 1,989,648 + GFF1965 0.12 +0.1 1,989,661 - GFF1965 0.14 +1.2 1,989,682 + GFF1965 0.18 +1.0 1,989,695 - GFF1965 0.20 +1.0 1,989,723 + GFF1965 0.25 -0.9 1,989,730 - GFF1965 0.26 -0.5 1,989,744 - GFF1965 0.28 +0.3 1,989,753 + GFF1965 0.30 -1.4 1,989,753 + GFF1965 0.30 -1.2 1,989,781 - GFF1965 0.35 -1.3 1,989,787 + GFF1965 0.36 -1.7 1,989,791 - GFF1965 0.36 -0.6 1,989,801 - GFF1965 0.38 -0.1 1,989,806 + GFF1965 0.39 -1.5 1,989,811 + GFF1965 0.40 -0.2 1,989,814 - GFF1965 0.40 -0.0 1,989,819 - GFF1965 0.41 -0.2 1,989,825 - GFF1965 0.42 -0.7 1,989,825 - GFF1965 0.42 +0.7 1,989,825 - GFF1965 0.42 -1.3 1,989,827 + GFF1965 0.42 +1.2 1,989,827 + GFF1965 0.42 -1.0
Or see this region's nucleotide sequence