Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_08440 and tolQ are separated by 8 nucleotides tolQ and OKGIIK_08450 are separated by 99 nucleotides OKGIIK_08450 and lolD are separated by 40 nucleotides lolD and OKGIIK_08460 overlap by 8 nucleotides
OKGIIK_08440: OKGIIK_08440 - Biopolymer transport protein ExbD, at 1,850,681 to 1,851,112
_08440
OKGIIK_08445: tolQ - biopolymer transporter ExbB, at 1,851,121 to 1,851,771
tolQ
OKGIIK_08450: OKGIIK_08450 - DNA internalization-related competence protein ComEC/Rec2, at 1,851,871 to 1,854,261
_08450
OKGIIK_08455: lolD - lipoprotein-releasing ABC transporter ATP-binding protein LolD, at 1,854,302 to 1,855,015
lolD
OKGIIK_08460: OKGIIK_08460 - lipoprotein-releasing ABC transporter permease subunit, at 1,855,008 to 1,856,249
_08460
Position (kb)
1851
1852
1853
1854
1855 Strain fitness (log2 ratio)
-2
-1
0
1 at 1851.267 kb on - strand, within tolQ at 1851.282 kb on - strand, within tolQ at 1851.282 kb on - strand, within tolQ at 1851.471 kb on - strand, within tolQ at 1851.647 kb on - strand, within tolQ at 1851.647 kb on - strand, within tolQ at 1851.647 kb on - strand, within tolQ at 1851.647 kb on - strand, within tolQ at 1851.647 kb on - strand, within tolQ at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.829 kb on + strand at 1851.830 kb on - strand at 1851.830 kb on - strand at 1851.830 kb on - strand at 1851.830 kb on - strand at 1851.830 kb on - strand at 1851.830 kb on - strand at 1851.830 kb on - strand at 1851.830 kb on - strand at 1851.885 kb on - strand at 1851.973 kb on - strand at 1851.973 kb on - strand at 1852.001 kb on + strand at 1852.001 kb on + strand at 1852.001 kb on + strand at 1852.002 kb on - strand at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.113 kb on + strand, within OKGIIK_08450 at 1852.114 kb on - strand, within OKGIIK_08450 at 1852.114 kb on - strand, within OKGIIK_08450 at 1852.114 kb on - strand, within OKGIIK_08450 at 1852.114 kb on - strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.556 kb on + strand, within OKGIIK_08450 at 1852.557 kb on - strand, within OKGIIK_08450 at 1852.557 kb on - strand, within OKGIIK_08450 at 1852.557 kb on - strand, within OKGIIK_08450 at 1852.557 kb on - strand, within OKGIIK_08450 at 1852.557 kb on - strand, within OKGIIK_08450 at 1852.588 kb on + strand, within OKGIIK_08450 at 1852.589 kb on - strand, within OKGIIK_08450 at 1852.781 kb on + strand, within OKGIIK_08450 at 1852.781 kb on + strand, within OKGIIK_08450 at 1852.781 kb on + strand, within OKGIIK_08450 at 1852.781 kb on + strand, within OKGIIK_08450 at 1852.781 kb on + strand, within OKGIIK_08450 at 1852.781 kb on + strand, within OKGIIK_08450 at 1852.782 kb on - strand, within OKGIIK_08450 at 1852.782 kb on - strand, within OKGIIK_08450 at 1852.782 kb on - strand, within OKGIIK_08450 at 1852.782 kb on - strand, within OKGIIK_08450 at 1852.782 kb on - strand, within OKGIIK_08450 at 1852.782 kb on - strand, within OKGIIK_08450 at 1853.262 kb on + strand, within OKGIIK_08450 at 1853.263 kb on - strand, within OKGIIK_08450 at 1853.263 kb on - strand, within OKGIIK_08450 at 1853.300 kb on + strand, within OKGIIK_08450 at 1853.301 kb on - strand, within OKGIIK_08450 at 1853.381 kb on + strand, within OKGIIK_08450 at 1853.381 kb on + strand, within OKGIIK_08450 at 1853.401 kb on + strand, within OKGIIK_08450 at 1853.402 kb on - strand, within OKGIIK_08450 at 1853.654 kb on + strand, within OKGIIK_08450 at 1853.654 kb on + strand, within OKGIIK_08450 at 1853.654 kb on + strand, within OKGIIK_08450 at 1853.654 kb on + strand, within OKGIIK_08450 at 1853.792 kb on + strand, within OKGIIK_08450 at 1853.792 kb on + strand, within OKGIIK_08450 at 1853.793 kb on - strand, within OKGIIK_08450 at 1853.793 kb on - strand, within OKGIIK_08450 at 1854.208 kb on + strand at 1854.209 kb on - strand at 1854.245 kb on + strand at 1854.246 kb on - strand at 1854.246 kb on - strand at 1854.246 kb on - strand at 1854.246 kb on - strand at 1854.246 kb on - strand at 1855.010 kb on - strand at 1855.010 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 1,851,267 - tolQ OKGIIK_08445 0.22 -1.0 1,851,282 - tolQ OKGIIK_08445 0.25 +0.4 1,851,282 - tolQ OKGIIK_08445 0.25 -1.2 1,851,471 - tolQ OKGIIK_08445 0.54 -2.6 1,851,647 - tolQ OKGIIK_08445 0.81 -0.7 1,851,647 - tolQ OKGIIK_08445 0.81 +0.1 1,851,647 - tolQ OKGIIK_08445 0.81 -0.5 1,851,647 - tolQ OKGIIK_08445 0.81 -1.3 1,851,647 - tolQ OKGIIK_08445 0.81 +0.0 1,851,829 + -1.1 1,851,829 + -2.3 1,851,829 + +0.6 1,851,829 + -0.1 1,851,829 + +0.0 1,851,829 + -2.0 1,851,829 + -0.3 1,851,829 + +0.7 1,851,829 + +0.1 1,851,829 + +0.6 1,851,829 + -0.7 1,851,829 + +0.1 1,851,829 + +0.7 1,851,830 - -0.3 1,851,830 - -1.5 1,851,830 - -0.7 1,851,830 - +0.3 1,851,830 - -0.3 1,851,830 - +1.3 1,851,830 - +1.0 1,851,830 - -0.9 1,851,885 - +0.7 1,851,973 - -0.8 1,851,973 - -1.5 1,852,001 + -0.4 1,852,001 + +0.5 1,852,001 + -1.9 1,852,002 - -0.8 1,852,113 + OKGIIK_08450 0.10 -1.3 1,852,113 + OKGIIK_08450 0.10 -0.7 1,852,113 + OKGIIK_08450 0.10 -0.7 1,852,113 + OKGIIK_08450 0.10 +0.9 1,852,113 + OKGIIK_08450 0.10 -0.5 1,852,113 + OKGIIK_08450 0.10 +0.6 1,852,113 + OKGIIK_08450 0.10 +0.4 1,852,113 + OKGIIK_08450 0.10 +0.1 1,852,114 - OKGIIK_08450 0.10 -0.1 1,852,114 - OKGIIK_08450 0.10 -0.5 1,852,114 - OKGIIK_08450 0.10 -0.0 1,852,114 - OKGIIK_08450 0.10 -1.8 1,852,556 + OKGIIK_08450 0.29 -1.0 1,852,556 + OKGIIK_08450 0.29 +0.6 1,852,556 + OKGIIK_08450 0.29 -1.0 1,852,556 + OKGIIK_08450 0.29 -0.8 1,852,556 + OKGIIK_08450 0.29 +1.1 1,852,556 + OKGIIK_08450 0.29 +0.2 1,852,556 + OKGIIK_08450 0.29 +0.9 1,852,556 + OKGIIK_08450 0.29 -1.6 1,852,557 - OKGIIK_08450 0.29 +0.8 1,852,557 - OKGIIK_08450 0.29 -1.5 1,852,557 - OKGIIK_08450 0.29 -0.5 1,852,557 - OKGIIK_08450 0.29 -1.7 1,852,557 - OKGIIK_08450 0.29 +0.1 1,852,588 + OKGIIK_08450 0.30 -1.0 1,852,589 - OKGIIK_08450 0.30 -1.4 1,852,781 + OKGIIK_08450 0.38 +0.6 1,852,781 + OKGIIK_08450 0.38 +0.3 1,852,781 + OKGIIK_08450 0.38 -0.0 1,852,781 + OKGIIK_08450 0.38 -0.3 1,852,781 + OKGIIK_08450 0.38 +0.9 1,852,781 + OKGIIK_08450 0.38 +0.1 1,852,782 - OKGIIK_08450 0.38 +0.4 1,852,782 - OKGIIK_08450 0.38 -0.3 1,852,782 - OKGIIK_08450 0.38 +0.7 1,852,782 - OKGIIK_08450 0.38 -0.7 1,852,782 - OKGIIK_08450 0.38 -1.0 1,852,782 - OKGIIK_08450 0.38 -1.5 1,853,262 + OKGIIK_08450 0.58 +0.4 1,853,263 - OKGIIK_08450 0.58 -0.0 1,853,263 - OKGIIK_08450 0.58 -0.7 1,853,300 + OKGIIK_08450 0.60 -1.5 1,853,301 - OKGIIK_08450 0.60 +0.0 1,853,381 + OKGIIK_08450 0.63 -1.0 1,853,381 + OKGIIK_08450 0.63 +0.0 1,853,401 + OKGIIK_08450 0.64 -1.0 1,853,402 - OKGIIK_08450 0.64 +0.1 1,853,654 + OKGIIK_08450 0.75 +0.4 1,853,654 + OKGIIK_08450 0.75 +1.5 1,853,654 + OKGIIK_08450 0.75 -0.6 1,853,654 + OKGIIK_08450 0.75 -0.2 1,853,792 + OKGIIK_08450 0.80 -2.7 1,853,792 + OKGIIK_08450 0.80 -0.7 1,853,793 - OKGIIK_08450 0.80 -0.9 1,853,793 - OKGIIK_08450 0.80 +0.3 1,854,208 + -0.5 1,854,209 - -0.1 1,854,245 + -0.9 1,854,246 - -0.0 1,854,246 - +0.8 1,854,246 - -1.4 1,854,246 - -1.3 1,854,246 - -0.9 1,855,010 - -0.8 1,855,010 - +1.2
Or see this region's nucleotide sequence