Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00300 and SGL_RS00305 are separated by 73 nucleotides SGL_RS00305 and SGL_RS19300 are separated by 329 nucleotides SGL_RS19300 and SGL_RS18490 overlap by 53 nucleotides SGL_RS18490 and SGL_RS00315 overlap by 74 nucleotides
SGL_RS00300: SGL_RS00300 - ParA family protein, at 67,362 to 67,976
_RS00300
SGL_RS00305: SGL_RS00305 - ATP-binding protein, at 68,050 to 68,574
_RS00305
SGL_RS19300: SGL_RS19300 - hypothetical protein, at 68,904 to 69,296
_RS19300
SGL_RS18490: SGL_RS18490 - hypothetical protein, at 69,244 to 69,408
_RS18490
SGL_RS00315: SGL_RS00315 - hypothetical protein, at 69,335 to 70,810
_RS00315
Position (kb) 68
69
70 Strain fitness (log2 ratio) -2
-1
0
1 at 67.947 kb on + strand at 68.309 kb on + strand, within SGL_RS00305 at 68.317 kb on - strand, within SGL_RS00305 at 68.317 kb on - strand, within SGL_RS00305 at 68.317 kb on - strand, within SGL_RS00305 at 68.317 kb on - strand, within SGL_RS00305 at 68.427 kb on - strand, within SGL_RS00305 at 68.548 kb on + strand at 68.564 kb on + strand at 68.564 kb on + strand at 68.582 kb on + strand at 68.582 kb on + strand at 68.590 kb on - strand at 68.590 kb on - strand at 68.590 kb on - strand at 68.660 kb on - strand at 68.694 kb on - strand at 68.882 kb on - strand at 68.888 kb on - strand at 68.967 kb on + strand, within SGL_RS19300 at 69.318 kb on + strand, within SGL_RS18490 at 69.479 kb on + strand at 69.479 kb on + strand at 69.497 kb on - strand, within SGL_RS00315 at 69.508 kb on + strand, within SGL_RS00315 at 69.637 kb on + strand, within SGL_RS00315
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 67,947 + +0.7 68,309 + SGL_RS00305 0.49 -0.0 68,317 - SGL_RS00305 0.51 -0.1 68,317 - SGL_RS00305 0.51 +0.1 68,317 - SGL_RS00305 0.51 +0.4 68,317 - SGL_RS00305 0.51 +0.9 68,427 - SGL_RS00305 0.72 +0.2 68,548 + -0.4 68,564 + -0.1 68,564 + +0.0 68,582 + +0.2 68,582 + +0.2 68,590 - -0.6 68,590 - -0.2 68,590 - +0.3 68,660 - +0.2 68,694 - -0.2 68,882 - +0.2 68,888 - -0.6 68,967 + SGL_RS19300 0.16 +0.3 69,318 + SGL_RS18490 0.45 -0.5 69,479 + -0.4 69,479 + -2.0 69,497 - SGL_RS00315 0.11 -0.0 69,508 + SGL_RS00315 0.12 +1.6 69,637 + SGL_RS00315 0.20 -0.9
Or see this region's nucleotide sequence