Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12735 and SGL_RS12740 are separated by 61 nucleotides SGL_RS12740 and SGL_RS12745 are separated by 385 nucleotides SGL_RS12745 and SGL_RS12750 are separated by 168 nucleotides
SGL_RS12735: SGL_RS12735 - virginiamycin B hydrolase Vgb, at 2,309,413 to 2,310,471
_RS12735
SGL_RS12740: SGL_RS12740 - hypothetical protein, at 2,310,533 to 2,310,991
_RS12740
SGL_RS12745: SGL_RS12745 - glycine cleavage system aminomethyltransferase GcvT, at 2,311,377 to 2,312,495
_RS12745
SGL_RS12750: SGL_RS12750 - molecular chaperone DnaK, at 2,312,664 to 2,314,574
_RS12750
Position (kb)
2311
2312
2313 Strain fitness (log2 ratio)
-2
-1
0
1 at 2310.456 kb on - strand at 2310.497 kb on - strand at 2310.578 kb on - strand at 2310.590 kb on - strand, within SGL_RS12740 at 2310.724 kb on + strand, within SGL_RS12740 at 2310.780 kb on + strand, within SGL_RS12740 at 2310.835 kb on + strand, within SGL_RS12740 at 2310.900 kb on - strand, within SGL_RS12740 at 2310.902 kb on - strand, within SGL_RS12740 at 2310.950 kb on + strand at 2310.958 kb on - strand at 2310.958 kb on - strand at 2311.152 kb on - strand at 2311.276 kb on + strand at 2311.528 kb on - strand, within SGL_RS12745 at 2311.528 kb on - strand, within SGL_RS12745 at 2311.690 kb on - strand, within SGL_RS12745 at 2311.799 kb on - strand, within SGL_RS12745 at 2311.810 kb on - strand, within SGL_RS12745 at 2311.825 kb on - strand, within SGL_RS12745 at 2312.217 kb on + strand, within SGL_RS12745 at 2312.224 kb on + strand, within SGL_RS12745 at 2312.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,310,456 - -0.4 2,310,497 - +0.6 2,310,578 - +0.0 2,310,590 - SGL_RS12740 0.12 +0.3 2,310,724 + SGL_RS12740 0.42 +0.3 2,310,780 + SGL_RS12740 0.54 -0.9 2,310,835 + SGL_RS12740 0.66 -0.3 2,310,900 - SGL_RS12740 0.80 -1.0 2,310,902 - SGL_RS12740 0.80 +0.6 2,310,950 + -0.8 2,310,958 - +0.5 2,310,958 - +0.3 2,311,152 - +0.6 2,311,276 + +0.3 2,311,528 - SGL_RS12745 0.13 -1.6 2,311,528 - SGL_RS12745 0.13 +0.9 2,311,690 - SGL_RS12745 0.28 -2.2 2,311,799 - SGL_RS12745 0.38 -2.2 2,311,810 - SGL_RS12745 0.39 -1.9 2,311,825 - SGL_RS12745 0.40 -1.6 2,312,217 + SGL_RS12745 0.75 -1.4 2,312,224 + SGL_RS12745 0.76 -2.1 2,312,592 - +0.5
Or see this region's nucleotide sequence