Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12470 and SGL_RS12475 are separated by 304 nucleotides SGL_RS12475 and SGL_RS12480 are separated by 242 nucleotides SGL_RS12480 and SGL_RS12485 are separated by 32 nucleotides
SGL_RS12470: SGL_RS12470 - DUF3318 domain-containing protein, at 2,253,169 to 2,253,807
_RS12470
SGL_RS12475: SGL_RS12475 - cation-translocating P-type ATPase, at 2,254,112 to 2,256,454
_RS12475
SGL_RS12480: SGL_RS12480 - FHA domain-containing protein, at 2,256,697 to 2,257,170
_RS12480
SGL_RS12485: SGL_RS12485 - YebC/PmpR family DNA-binding transcriptional regulator, at 2,257,203 to 2,257,964
_RS12485
Position (kb)
2254
2255
2256
2257 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2253.816 kb on - strand at 2253.845 kb on - strand at 2254.191 kb on + strand at 2254.192 kb on - strand at 2254.406 kb on - strand, within SGL_RS12475 at 2254.480 kb on + strand, within SGL_RS12475 at 2254.540 kb on + strand, within SGL_RS12475 at 2254.584 kb on - strand, within SGL_RS12475 at 2254.791 kb on - strand, within SGL_RS12475 at 2254.806 kb on + strand, within SGL_RS12475 at 2254.919 kb on + strand, within SGL_RS12475 at 2255.099 kb on + strand, within SGL_RS12475 at 2255.107 kb on - strand, within SGL_RS12475 at 2255.210 kb on + strand, within SGL_RS12475 at 2255.477 kb on + strand, within SGL_RS12475 at 2255.567 kb on - strand, within SGL_RS12475 at 2255.629 kb on - strand, within SGL_RS12475 at 2255.758 kb on - strand, within SGL_RS12475 at 2255.958 kb on - strand, within SGL_RS12475 at 2256.115 kb on + strand, within SGL_RS12475 at 2256.115 kb on + strand, within SGL_RS12475 at 2256.118 kb on - strand, within SGL_RS12475 at 2256.219 kb on + strand, within SGL_RS12475 at 2256.245 kb on - strand at 2256.406 kb on + strand at 2256.777 kb on + strand, within SGL_RS12480 at 2256.933 kb on - strand, within SGL_RS12480 at 2257.053 kb on + strand, within SGL_RS12480 at 2257.063 kb on + strand, within SGL_RS12480 at 2257.233 kb on - strand at 2257.316 kb on - strand, within SGL_RS12485 at 2257.321 kb on - strand, within SGL_RS12485 at 2257.426 kb on + strand, within SGL_RS12485 at 2257.434 kb on - strand, within SGL_RS12485
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 2,253,816 - -1.0 2,253,845 - -0.0 2,254,191 + -2.7 2,254,192 - -3.4 2,254,406 - SGL_RS12475 0.13 -5.8 2,254,480 + SGL_RS12475 0.16 -0.7 2,254,540 + SGL_RS12475 0.18 -4.9 2,254,584 - SGL_RS12475 0.20 -5.0 2,254,791 - SGL_RS12475 0.29 -2.7 2,254,806 + SGL_RS12475 0.30 -3.2 2,254,919 + SGL_RS12475 0.34 -1.4 2,255,099 + SGL_RS12475 0.42 -3.6 2,255,107 - SGL_RS12475 0.42 -1.6 2,255,210 + SGL_RS12475 0.47 -4.0 2,255,477 + SGL_RS12475 0.58 -3.2 2,255,567 - SGL_RS12475 0.62 -3.9 2,255,629 - SGL_RS12475 0.65 -5.5 2,255,758 - SGL_RS12475 0.70 -1.1 2,255,958 - SGL_RS12475 0.79 -4.7 2,256,115 + SGL_RS12475 0.85 -2.6 2,256,115 + SGL_RS12475 0.85 -5.0 2,256,118 - SGL_RS12475 0.86 -4.1 2,256,219 + SGL_RS12475 0.90 -3.6 2,256,245 - -4.7 2,256,406 + +0.6 2,256,777 + SGL_RS12480 0.17 +0.0 2,256,933 - SGL_RS12480 0.50 -1.1 2,257,053 + SGL_RS12480 0.75 -0.1 2,257,063 + SGL_RS12480 0.77 -1.2 2,257,233 - -0.1 2,257,316 - SGL_RS12485 0.15 -0.3 2,257,321 - SGL_RS12485 0.15 -0.5 2,257,426 + SGL_RS12485 0.29 -1.3 2,257,434 - SGL_RS12485 0.30 -0.5
Or see this region's nucleotide sequence