Experiment: R2A_PIPES with Cobalt chloride 512 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_03495 and yajQ are separated by 108 nucleotides yajQ and yhiN are separated by 89 nucleotides yhiN and mmsB are separated by 84 nucleotides
OKGIIK_03495: OKGIIK_03495 - hypothetical protein, at 777,100 to 777,771
_03495
OKGIIK_03500: yajQ - YajQ family cyclic di-GMP-binding protein, at 777,880 to 778,362
yajQ
OKGIIK_03505: yhiN - NAD(P)/FAD-dependent oxidoreductase, at 778,452 to 779,630
yhiN
OKGIIK_03510: mmsB - 3-hydroxyisobutyrate dehydrogenase or related beta-hydroxyacid dehydrogenase, at 779,715 to 780,599
mmsB
Position (kb) 778
779
780 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 777.722 kb on + strand at 777.722 kb on + strand at 777.723 kb on - strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.788 kb on + strand at 777.789 kb on - strand at 777.789 kb on - strand at 777.789 kb on - strand at 777.789 kb on - strand at 777.789 kb on - strand at 777.789 kb on - strand at 777.789 kb on - strand at 777.789 kb on - strand at 777.861 kb on + strand at 778.247 kb on + strand, within yajQ at 778.247 kb on + strand, within yajQ at 778.247 kb on + strand, within yajQ at 778.247 kb on + strand, within yajQ at 778.247 kb on + strand, within yajQ at 778.247 kb on + strand, within yajQ at 778.247 kb on + strand, within yajQ at 778.247 kb on + strand, within yajQ at 778.248 kb on - strand, within yajQ at 778.248 kb on - strand, within yajQ at 778.248 kb on - strand, within yajQ at 778.248 kb on - strand, within yajQ at 778.248 kb on - strand, within yajQ at 778.248 kb on - strand, within yajQ at 778.395 kb on + strand at 778.395 kb on + strand at 778.395 kb on + strand at 778.395 kb on + strand at 778.396 kb on - strand at 778.396 kb on - strand at 778.396 kb on - strand at 778.396 kb on - strand at 778.396 kb on - strand at 778.396 kb on - strand at 778.396 kb on - strand at 778.396 kb on - strand at 778.416 kb on - strand at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.632 kb on + strand, within yhiN at 778.633 kb on - strand, within yhiN at 778.633 kb on - strand, within yhiN at 778.633 kb on - strand, within yhiN at 778.633 kb on - strand, within yhiN at 778.677 kb on + strand, within yhiN at 778.725 kb on + strand, within yhiN at 778.726 kb on - strand, within yhiN at 779.058 kb on + strand, within yhiN at 779.059 kb on - strand, within yhiN at 779.059 kb on - strand, within yhiN at 779.184 kb on + strand, within yhiN at 779.185 kb on - strand, within yhiN at 779.532 kb on + strand at 779.532 kb on + strand at 779.532 kb on + strand at 779.532 kb on + strand at 779.532 kb on + strand at 779.532 kb on + strand at 779.532 kb on + strand at 779.533 kb on - strand at 779.533 kb on - strand at 779.533 kb on - strand at 779.533 kb on - strand at 779.533 kb on - strand at 779.533 kb on - strand at 779.577 kb on + strand at 779.577 kb on + strand at 779.578 kb on - strand at 779.578 kb on - strand at 779.668 kb on - strand at 780.052 kb on - strand, within mmsB at 780.052 kb on - strand, within mmsB at 780.052 kb on - strand, within mmsB at 780.052 kb on - strand, within mmsB at 780.052 kb on - strand, within mmsB at 780.068 kb on - strand, within mmsB at 780.107 kb on + strand, within mmsB at 780.108 kb on - strand, within mmsB at 780.214 kb on + strand, within mmsB at 780.214 kb on + strand, within mmsB at 780.214 kb on + strand, within mmsB at 780.215 kb on - strand, within mmsB at 780.291 kb on + strand, within mmsB at 780.292 kb on - strand, within mmsB at 780.354 kb on + strand, within mmsB at 780.627 kb on - strand at 780.627 kb on - strand at 780.627 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cobalt chloride 512 uM remove 777,722 + +0.1 777,722 + +0.7 777,723 - -1.2 777,788 + +0.0 777,788 + -0.4 777,788 + -0.3 777,788 + +0.6 777,788 + -0.6 777,788 + +0.5 777,788 + +0.5 777,788 + -0.0 777,788 + +1.1 777,789 - +0.0 777,789 - +1.9 777,789 - -1.1 777,789 - +0.4 777,789 - +0.8 777,789 - +0.1 777,789 - +0.6 777,789 - -0.8 777,861 + +0.0 778,247 + yajQ OKGIIK_03500 0.76 -1.5 778,247 + yajQ OKGIIK_03500 0.76 -1.0 778,247 + yajQ OKGIIK_03500 0.76 +2.2 778,247 + yajQ OKGIIK_03500 0.76 -1.7 778,247 + yajQ OKGIIK_03500 0.76 +1.0 778,247 + yajQ OKGIIK_03500 0.76 -0.2 778,247 + yajQ OKGIIK_03500 0.76 -0.2 778,247 + yajQ OKGIIK_03500 0.76 +1.6 778,248 - yajQ OKGIIK_03500 0.76 +0.9 778,248 - yajQ OKGIIK_03500 0.76 -0.6 778,248 - yajQ OKGIIK_03500 0.76 +0.1 778,248 - yajQ OKGIIK_03500 0.76 -1.5 778,248 - yajQ OKGIIK_03500 0.76 -0.6 778,248 - yajQ OKGIIK_03500 0.76 -0.2 778,395 + -0.8 778,395 + -0.6 778,395 + -0.8 778,395 + -0.6 778,396 - +1.7 778,396 - +0.6 778,396 - +0.8 778,396 - +0.1 778,396 - -1.2 778,396 - -2.1 778,396 - +0.2 778,396 - -1.0 778,416 - +1.0 778,632 + yhiN OKGIIK_03505 0.15 +1.7 778,632 + yhiN OKGIIK_03505 0.15 -0.9 778,632 + yhiN OKGIIK_03505 0.15 -0.8 778,632 + yhiN OKGIIK_03505 0.15 +2.0 778,632 + yhiN OKGIIK_03505 0.15 +0.6 778,632 + yhiN OKGIIK_03505 0.15 -0.1 778,632 + yhiN OKGIIK_03505 0.15 -0.2 778,632 + yhiN OKGIIK_03505 0.15 -1.6 778,632 + yhiN OKGIIK_03505 0.15 +0.8 778,632 + yhiN OKGIIK_03505 0.15 -2.2 778,632 + yhiN OKGIIK_03505 0.15 -2.1 778,632 + yhiN OKGIIK_03505 0.15 -0.6 778,633 - yhiN OKGIIK_03505 0.15 +0.4 778,633 - yhiN OKGIIK_03505 0.15 +0.7 778,633 - yhiN OKGIIK_03505 0.15 +0.5 778,633 - yhiN OKGIIK_03505 0.15 -0.6 778,677 + yhiN OKGIIK_03505 0.19 +0.9 778,725 + yhiN OKGIIK_03505 0.23 -0.0 778,726 - yhiN OKGIIK_03505 0.23 -1.0 779,058 + yhiN OKGIIK_03505 0.51 -0.6 779,059 - yhiN OKGIIK_03505 0.51 +0.3 779,059 - yhiN OKGIIK_03505 0.51 -0.1 779,184 + yhiN OKGIIK_03505 0.62 -1.5 779,185 - yhiN OKGIIK_03505 0.62 -2.0 779,532 + -0.9 779,532 + +0.1 779,532 + -0.4 779,532 + +1.2 779,532 + -1.3 779,532 + +0.8 779,532 + -0.2 779,533 - +1.4 779,533 - -0.5 779,533 - -1.5 779,533 - -0.1 779,533 - -0.4 779,533 - -3.6 779,577 + +0.4 779,577 + +0.8 779,578 - -0.8 779,578 - +1.4 779,668 - +0.4 780,052 - mmsB OKGIIK_03510 0.38 -0.2 780,052 - mmsB OKGIIK_03510 0.38 +0.1 780,052 - mmsB OKGIIK_03510 0.38 +1.2 780,052 - mmsB OKGIIK_03510 0.38 -0.2 780,052 - mmsB OKGIIK_03510 0.38 +0.1 780,068 - mmsB OKGIIK_03510 0.40 +0.4 780,107 + mmsB OKGIIK_03510 0.44 -1.1 780,108 - mmsB OKGIIK_03510 0.44 -0.5 780,214 + mmsB OKGIIK_03510 0.56 -0.8 780,214 + mmsB OKGIIK_03510 0.56 -1.0 780,214 + mmsB OKGIIK_03510 0.56 -0.6 780,215 - mmsB OKGIIK_03510 0.56 -1.2 780,291 + mmsB OKGIIK_03510 0.65 -2.6 780,292 - mmsB OKGIIK_03510 0.65 +1.4 780,354 + mmsB OKGIIK_03510 0.72 -0.6 780,627 - -1.1 780,627 - -0.1 780,627 - +1.4
Or see this region's nucleotide sequence