Experiment: Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1305 and GFF1306 are separated by 289 nucleotides GFF1306 and GFF1307 are separated by 39 nucleotides GFF1307 and GFF1308 are separated by 40 nucleotides
GFF1305 - hypothetical protein, at 61,306 to 62,262
GFF1305
GFF1306 - putative low molecular weight protein-tyrosine-phosphatase AmsI, at 62,552 to 62,992
GFF1306
GFF1307 - Tyrosine-protein kinase wzc, at 63,032 to 65,254
GFF1307
GFF1308 - D-inositol-3-phosphate glycosyltransferase, at 65,295 to 66,377
GFF1308
Position (kb) 63
64
65
66 Strain fitness (log2 ratio) -2
-1
0
1 at 62.036 kb on + strand, within GFF1305 at 62.036 kb on + strand, within GFF1305 at 62.036 kb on + strand, within GFF1305 at 62.036 kb on + strand, within GFF1305 at 62.036 kb on + strand, within GFF1305 at 62.037 kb on - strand, within GFF1305 at 62.037 kb on - strand, within GFF1305 at 62.241 kb on + strand at 62.290 kb on + strand at 62.291 kb on - strand at 62.297 kb on - strand at 62.297 kb on - strand at 62.297 kb on - strand at 62.337 kb on + strand at 62.442 kb on - strand at 62.455 kb on + strand at 62.535 kb on - strand at 62.587 kb on + strand at 62.587 kb on + strand at 62.587 kb on + strand at 62.588 kb on - strand at 62.588 kb on - strand at 62.589 kb on + strand at 62.589 kb on + strand at 62.589 kb on + strand at 62.589 kb on + strand at 62.590 kb on - strand at 62.590 kb on - strand at 62.648 kb on - strand, within GFF1306 at 62.895 kb on + strand, within GFF1306 at 63.012 kb on + strand at 63.031 kb on + strand at 63.032 kb on - strand at 63.040 kb on - strand at 63.079 kb on + strand at 63.079 kb on + strand at 63.079 kb on + strand at 63.194 kb on + strand at 63.195 kb on - strand at 63.195 kb on - strand at 63.467 kb on + strand, within GFF1307 at 63.468 kb on - strand, within GFF1307 at 63.639 kb on + strand, within GFF1307 at 63.639 kb on + strand, within GFF1307 at 63.640 kb on - strand, within GFF1307 at 63.640 kb on - strand, within GFF1307 at 63.640 kb on - strand, within GFF1307 at 63.691 kb on - strand, within GFF1307 at 63.716 kb on - strand, within GFF1307 at 63.716 kb on - strand, within GFF1307 at 63.842 kb on + strand, within GFF1307 at 63.843 kb on - strand, within GFF1307 at 64.107 kb on - strand, within GFF1307 at 64.242 kb on - strand, within GFF1307 at 64.242 kb on - strand, within GFF1307 at 64.242 kb on - strand, within GFF1307 at 64.488 kb on - strand, within GFF1307 at 64.571 kb on + strand, within GFF1307 at 64.948 kb on + strand, within GFF1307 at 64.948 kb on + strand, within GFF1307 at 64.948 kb on + strand, within GFF1307 at 64.949 kb on - strand, within GFF1307 at 64.949 kb on - strand, within GFF1307 at 64.949 kb on - strand, within GFF1307 at 64.949 kb on - strand, within GFF1307 at 65.202 kb on - strand at 65.202 kb on - strand at 65.204 kb on + strand at 65.205 kb on - strand at 65.219 kb on + strand at 65.222 kb on + strand at 65.222 kb on + strand at 65.222 kb on + strand at 65.222 kb on + strand at 65.222 kb on + strand at 65.222 kb on + strand at 65.223 kb on - strand at 65.223 kb on - strand at 65.223 kb on - strand at 65.223 kb on - strand at 65.223 kb on - strand at 65.228 kb on + strand at 65.228 kb on + strand at 65.229 kb on - strand at 65.229 kb on - strand at 65.234 kb on + strand at 65.235 kb on - strand at 65.392 kb on - strand at 65.403 kb on + strand at 65.404 kb on - strand, within GFF1308 at 65.404 kb on - strand, within GFF1308 at 65.637 kb on + strand, within GFF1308 at 65.694 kb on + strand, within GFF1308 at 65.765 kb on + strand, within GFF1308 at 65.766 kb on - strand, within GFF1308 at 65.766 kb on - strand, within GFF1308 at 65.811 kb on + strand, within GFF1308 at 65.811 kb on + strand, within GFF1308 at 65.812 kb on - strand, within GFF1308 at 65.812 kb on - strand, within GFF1308 at 65.812 kb on - strand, within GFF1308 at 66.025 kb on - strand, within GFF1308 at 66.063 kb on + strand, within GFF1308 at 66.064 kb on - strand, within GFF1308 at 66.207 kb on + strand, within GFF1308
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Methylosinus sp. DMC45 1:1 with Pseudomonas_DMC3_ML3; dNMS with methane remove 62,036 + GFF1305 0.76 +0.8 62,036 + GFF1305 0.76 -0.5 62,036 + GFF1305 0.76 +0.5 62,036 + GFF1305 0.76 +0.0 62,036 + GFF1305 0.76 +0.8 62,037 - GFF1305 0.76 +0.1 62,037 - GFF1305 0.76 +0.2 62,241 + -0.5 62,290 + -0.6 62,291 - -0.3 62,297 - -0.8 62,297 - +0.5 62,297 - +0.0 62,337 + -0.6 62,442 - -0.5 62,455 + -1.0 62,535 - +0.6 62,587 + -0.1 62,587 + -1.1 62,587 + -0.6 62,588 - -0.9 62,588 - -0.7 62,589 + +0.6 62,589 + +1.0 62,589 + -0.8 62,589 + +0.6 62,590 - +0.5 62,590 - -2.0 62,648 - GFF1306 0.22 +0.2 62,895 + GFF1306 0.78 +0.5 63,012 + -1.0 63,031 + +0.2 63,032 - +0.3 63,040 - -0.4 63,079 + -0.4 63,079 + -0.8 63,079 + +1.1 63,194 + +0.2 63,195 - -0.0 63,195 - -0.1 63,467 + GFF1307 0.20 -0.6 63,468 - GFF1307 0.20 +0.6 63,639 + GFF1307 0.27 +0.8 63,639 + GFF1307 0.27 -1.4 63,640 - GFF1307 0.27 +0.4 63,640 - GFF1307 0.27 -1.1 63,640 - GFF1307 0.27 -0.3 63,691 - GFF1307 0.30 -0.5 63,716 - GFF1307 0.31 -0.7 63,716 - GFF1307 0.31 -0.8 63,842 + GFF1307 0.36 +0.0 63,843 - GFF1307 0.36 -1.2 64,107 - GFF1307 0.48 -1.4 64,242 - GFF1307 0.54 -0.2 64,242 - GFF1307 0.54 +0.8 64,242 - GFF1307 0.54 +1.1 64,488 - GFF1307 0.65 +1.0 64,571 + GFF1307 0.69 -0.2 64,948 + GFF1307 0.86 -0.3 64,948 + GFF1307 0.86 +0.9 64,948 + GFF1307 0.86 +0.1 64,949 - GFF1307 0.86 -0.6 64,949 - GFF1307 0.86 -0.1 64,949 - GFF1307 0.86 -0.9 64,949 - GFF1307 0.86 -1.4 65,202 - +0.4 65,202 - -0.1 65,204 + +0.8 65,205 - -0.2 65,219 + +0.9 65,222 + -0.2 65,222 + +0.6 65,222 + -1.1 65,222 + -0.5 65,222 + +0.6 65,222 + +0.6 65,223 - -0.6 65,223 - -0.2 65,223 - -0.7 65,223 - -1.2 65,223 - +0.2 65,228 + +0.5 65,228 + +0.4 65,229 - -1.2 65,229 - +0.5 65,234 + -0.6 65,235 - +0.3 65,392 - +0.2 65,403 + -1.0 65,404 - GFF1308 0.10 +1.0 65,404 - GFF1308 0.10 -1.0 65,637 + GFF1308 0.32 -0.3 65,694 + GFF1308 0.37 -1.1 65,765 + GFF1308 0.43 -0.5 65,766 - GFF1308 0.43 -1.1 65,766 - GFF1308 0.43 -0.6 65,811 + GFF1308 0.48 -0.2 65,811 + GFF1308 0.48 +0.4 65,812 - GFF1308 0.48 -1.8 65,812 - GFF1308 0.48 +1.2 65,812 - GFF1308 0.48 -0.4 66,025 - GFF1308 0.67 -0.6 66,063 + GFF1308 0.71 +0.2 66,064 - GFF1308 0.71 +0.5 66,207 + GFF1308 0.84 -0.6
Or see this region's nucleotide sequence