Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15565 and SGL_RS15570 are separated by 89 nucleotides SGL_RS15570 and SGL_RS15575 are separated by 116 nucleotides SGL_RS15575 and SGL_RS15580 are separated by 35 nucleotides
SGL_RS15565: SGL_RS15565 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 2,940,566 to 2,941,666
_RS15565
SGL_RS15570: SGL_RS15570 - AI-2E family transporter, at 2,941,756 to 2,942,883
_RS15570
SGL_RS15575: SGL_RS15575 - creatininase family protein, at 2,943,000 to 2,943,734
_RS15575
SGL_RS15580: SGL_RS15580 - hypothetical protein, at 2,943,770 to 2,944,201
_RS15580
Position (kb)
2941
2942
2943 Strain fitness (log2 ratio)
-2
-1
0
1 at 2940.802 kb on + strand, within SGL_RS15565 at 2940.845 kb on + strand, within SGL_RS15565 at 2940.853 kb on + strand, within SGL_RS15565 at 2941.006 kb on + strand, within SGL_RS15565 at 2941.166 kb on + strand, within SGL_RS15565 at 2941.280 kb on - strand, within SGL_RS15565 at 2941.296 kb on + strand, within SGL_RS15565 at 2941.304 kb on - strand, within SGL_RS15565 at 2941.601 kb on + strand at 2941.728 kb on + strand at 2941.735 kb on - strand at 2941.736 kb on - strand at 2941.736 kb on - strand at 2941.878 kb on - strand, within SGL_RS15570 at 2942.065 kb on + strand, within SGL_RS15570 at 2942.143 kb on - strand, within SGL_RS15570 at 2942.442 kb on - strand, within SGL_RS15570 at 2942.461 kb on - strand, within SGL_RS15570 at 2942.488 kb on - strand, within SGL_RS15570 at 2942.619 kb on + strand, within SGL_RS15570 at 2942.631 kb on + strand, within SGL_RS15570 at 2942.786 kb on + strand at 2942.808 kb on + strand at 2942.819 kb on + strand at 2942.900 kb on - strand at 2943.069 kb on + strand at 2943.082 kb on - strand, within SGL_RS15575 at 2943.198 kb on - strand, within SGL_RS15575 at 2943.265 kb on + strand, within SGL_RS15575 at 2943.273 kb on - strand, within SGL_RS15575 at 2943.273 kb on - strand, within SGL_RS15575 at 2943.465 kb on - strand, within SGL_RS15575 at 2943.497 kb on + strand, within SGL_RS15575 at 2943.624 kb on + strand, within SGL_RS15575 at 2943.627 kb on - strand, within SGL_RS15575 at 2943.632 kb on - strand, within SGL_RS15575 at 2943.881 kb on - strand, within SGL_RS15580
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,940,802 + SGL_RS15565 0.21 +0.1 2,940,845 + SGL_RS15565 0.25 -0.5 2,940,853 + SGL_RS15565 0.26 +0.2 2,941,006 + SGL_RS15565 0.40 +0.6 2,941,166 + SGL_RS15565 0.54 -0.2 2,941,280 - SGL_RS15565 0.65 +0.5 2,941,296 + SGL_RS15565 0.66 +0.0 2,941,304 - SGL_RS15565 0.67 +0.7 2,941,601 + +0.2 2,941,728 + -0.5 2,941,735 - +0.1 2,941,736 - -0.6 2,941,736 - -0.2 2,941,878 - SGL_RS15570 0.11 -0.6 2,942,065 + SGL_RS15570 0.27 -2.2 2,942,143 - SGL_RS15570 0.34 -1.0 2,942,442 - SGL_RS15570 0.61 -1.3 2,942,461 - SGL_RS15570 0.62 -0.2 2,942,488 - SGL_RS15570 0.65 -0.6 2,942,619 + SGL_RS15570 0.77 -0.8 2,942,631 + SGL_RS15570 0.78 -0.8 2,942,786 + -0.4 2,942,808 + -0.5 2,942,819 + -1.7 2,942,900 - -0.6 2,943,069 + -0.4 2,943,082 - SGL_RS15575 0.11 -0.4 2,943,198 - SGL_RS15575 0.27 -0.8 2,943,265 + SGL_RS15575 0.36 -0.5 2,943,273 - SGL_RS15575 0.37 -0.2 2,943,273 - SGL_RS15575 0.37 -0.2 2,943,465 - SGL_RS15575 0.63 -0.8 2,943,497 + SGL_RS15575 0.68 +0.2 2,943,624 + SGL_RS15575 0.85 +0.5 2,943,627 - SGL_RS15575 0.85 -0.5 2,943,632 - SGL_RS15575 0.86 +0.2 2,943,881 - SGL_RS15580 0.26 -0.0
Or see this region's nucleotide sequence