Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03750 and SGL_RS03755 overlap by 27 nucleotides SGL_RS03755 and SGL_RS03760 overlap by 1 nucleotides SGL_RS03760 and SGL_RS03765 are separated by 270 nucleotides
SGL_RS03750: SGL_RS03750 - esterase-like activity of phytase family protein, at 388,065 to 389,201
_RS03750
SGL_RS03755: SGL_RS03755 - FAD-binding oxidoreductase, at 389,175 to 390,353
_RS03755
SGL_RS03760: SGL_RS03760 - hypothetical protein, at 390,353 to 390,634
_RS03760
SGL_RS03765: SGL_RS03765 - bestrophin family protein, at 390,905 to 391,840
_RS03765
Position (kb) 389
390
391 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 388.195 kb on + strand, within SGL_RS03750 at 388.203 kb on - strand, within SGL_RS03750 at 388.251 kb on - strand, within SGL_RS03750 at 388.313 kb on + strand, within SGL_RS03750 at 388.313 kb on + strand, within SGL_RS03750 at 388.321 kb on - strand, within SGL_RS03750 at 388.321 kb on - strand, within SGL_RS03750 at 388.326 kb on - strand, within SGL_RS03750 at 388.333 kb on + strand, within SGL_RS03750 at 388.352 kb on - strand, within SGL_RS03750 at 388.394 kb on + strand, within SGL_RS03750 at 388.574 kb on - strand, within SGL_RS03750 at 388.601 kb on + strand, within SGL_RS03750 at 388.610 kb on - strand, within SGL_RS03750 at 388.704 kb on + strand, within SGL_RS03750 at 388.802 kb on - strand, within SGL_RS03750 at 388.811 kb on + strand, within SGL_RS03750 at 388.908 kb on - strand, within SGL_RS03750 at 388.912 kb on + strand, within SGL_RS03750 at 389.011 kb on + strand, within SGL_RS03750 at 389.099 kb on - strand at 389.217 kb on + strand at 389.357 kb on - strand, within SGL_RS03755 at 389.380 kb on - strand, within SGL_RS03755 at 389.382 kb on + strand, within SGL_RS03755 at 389.455 kb on + strand, within SGL_RS03755 at 389.463 kb on - strand, within SGL_RS03755 at 389.542 kb on + strand, within SGL_RS03755 at 389.550 kb on - strand, within SGL_RS03755 at 389.574 kb on - strand, within SGL_RS03755 at 389.589 kb on - strand, within SGL_RS03755 at 389.625 kb on - strand, within SGL_RS03755 at 389.638 kb on - strand, within SGL_RS03755 at 389.683 kb on + strand, within SGL_RS03755 at 389.710 kb on - strand, within SGL_RS03755 at 389.744 kb on + strand, within SGL_RS03755 at 389.752 kb on - strand, within SGL_RS03755 at 389.759 kb on - strand, within SGL_RS03755 at 389.889 kb on + strand, within SGL_RS03755 at 389.996 kb on + strand, within SGL_RS03755 at 389.996 kb on + strand, within SGL_RS03755 at 390.053 kb on + strand, within SGL_RS03755 at 390.179 kb on + strand, within SGL_RS03755 at 390.179 kb on + strand, within SGL_RS03755 at 390.185 kb on - strand, within SGL_RS03755 at 390.219 kb on + strand, within SGL_RS03755 at 390.219 kb on + strand, within SGL_RS03755 at 390.219 kb on + strand, within SGL_RS03755 at 390.449 kb on - strand, within SGL_RS03760 at 390.569 kb on - strand, within SGL_RS03760 at 390.590 kb on + strand, within SGL_RS03760 at 390.591 kb on - strand, within SGL_RS03760 at 390.962 kb on + strand at 390.965 kb on + strand at 390.970 kb on - strand at 391.103 kb on - strand, within SGL_RS03765 at 391.175 kb on + strand, within SGL_RS03765 at 391.183 kb on - strand, within SGL_RS03765 at 391.227 kb on + strand, within SGL_RS03765
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 388,195 + SGL_RS03750 0.11 +0.2 388,203 - SGL_RS03750 0.12 +0.2 388,251 - SGL_RS03750 0.16 +0.3 388,313 + SGL_RS03750 0.22 -0.5 388,313 + SGL_RS03750 0.22 -0.5 388,321 - SGL_RS03750 0.23 +0.0 388,321 - SGL_RS03750 0.23 +0.3 388,326 - SGL_RS03750 0.23 -1.0 388,333 + SGL_RS03750 0.24 +0.3 388,352 - SGL_RS03750 0.25 -0.9 388,394 + SGL_RS03750 0.29 -0.8 388,574 - SGL_RS03750 0.45 -0.3 388,601 + SGL_RS03750 0.47 +0.2 388,610 - SGL_RS03750 0.48 +0.1 388,704 + SGL_RS03750 0.56 -0.2 388,802 - SGL_RS03750 0.65 +0.4 388,811 + SGL_RS03750 0.66 +0.3 388,908 - SGL_RS03750 0.74 +1.2 388,912 + SGL_RS03750 0.74 +0.1 389,011 + SGL_RS03750 0.83 +0.3 389,099 - +0.1 389,217 + -2.8 389,357 - SGL_RS03755 0.15 -0.7 389,380 - SGL_RS03755 0.17 -2.5 389,382 + SGL_RS03755 0.18 -2.6 389,455 + SGL_RS03755 0.24 -1.0 389,463 - SGL_RS03755 0.24 -1.7 389,542 + SGL_RS03755 0.31 -0.5 389,550 - SGL_RS03755 0.32 -3.5 389,574 - SGL_RS03755 0.34 -2.7 389,589 - SGL_RS03755 0.35 -2.5 389,625 - SGL_RS03755 0.38 -2.3 389,638 - SGL_RS03755 0.39 -1.2 389,683 + SGL_RS03755 0.43 -2.5 389,710 - SGL_RS03755 0.45 -1.8 389,744 + SGL_RS03755 0.48 -2.5 389,752 - SGL_RS03755 0.49 -2.0 389,759 - SGL_RS03755 0.50 -3.7 389,889 + SGL_RS03755 0.61 -1.0 389,996 + SGL_RS03755 0.70 +0.6 389,996 + SGL_RS03755 0.70 -2.5 390,053 + SGL_RS03755 0.74 -1.6 390,179 + SGL_RS03755 0.85 -1.7 390,179 + SGL_RS03755 0.85 -2.5 390,185 - SGL_RS03755 0.86 -2.0 390,219 + SGL_RS03755 0.89 -2.4 390,219 + SGL_RS03755 0.89 -0.5 390,219 + SGL_RS03755 0.89 +1.4 390,449 - SGL_RS03760 0.34 -1.4 390,569 - SGL_RS03760 0.77 -0.8 390,590 + SGL_RS03760 0.84 -1.9 390,591 - SGL_RS03760 0.84 -2.0 390,962 + -0.8 390,965 + -0.8 390,970 - -0.1 391,103 - SGL_RS03765 0.21 -0.2 391,175 + SGL_RS03765 0.29 -0.8 391,183 - SGL_RS03765 0.30 -0.2 391,227 + SGL_RS03765 0.34 -0.4
Or see this region's nucleotide sequence