Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02145 and SGL_RS02150 are separated by 72 nucleotides SGL_RS02150 and SGL_RS02155 are separated by 38 nucleotides SGL_RS02155 and SGL_RS02160 are separated by 224 nucleotides
SGL_RS02145: SGL_RS02145 - metallophosphoesterase, at 65,827 to 67,533
_RS02145
SGL_RS02150: SGL_RS02150 - WecB/TagA/CpsF family glycosyltransferase, at 67,606 to 68,361
_RS02150
SGL_RS02155: SGL_RS02155 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 68,400 to 69,059
_RS02155
SGL_RS02160: SGL_RS02160 - GDSL-type esterase/lipase family protein, at 69,284 to 69,985
_RS02160
Position (kb) 67
68
69 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 66.671 kb on - strand, within SGL_RS02145 at 66.752 kb on - strand, within SGL_RS02145 at 66.757 kb on - strand, within SGL_RS02145 at 66.785 kb on + strand, within SGL_RS02145 at 66.785 kb on + strand, within SGL_RS02145 at 66.829 kb on - strand, within SGL_RS02145 at 66.835 kb on + strand, within SGL_RS02145 at 66.877 kb on - strand, within SGL_RS02145 at 66.972 kb on - strand, within SGL_RS02145 at 66.999 kb on + strand, within SGL_RS02145 at 67.043 kb on - strand, within SGL_RS02145 at 67.087 kb on - strand, within SGL_RS02145 at 67.163 kb on - strand, within SGL_RS02145 at 67.166 kb on + strand, within SGL_RS02145 at 67.200 kb on - strand, within SGL_RS02145 at 67.260 kb on - strand, within SGL_RS02145 at 67.322 kb on + strand, within SGL_RS02145 at 67.330 kb on - strand, within SGL_RS02145 at 67.330 kb on - strand, within SGL_RS02145 at 67.330 kb on - strand, within SGL_RS02145 at 67.399 kb on + strand at 67.399 kb on + strand at 67.406 kb on + strand at 67.463 kb on - strand at 67.657 kb on + strand at 67.660 kb on - strand at 67.786 kb on + strand, within SGL_RS02150 at 67.794 kb on - strand, within SGL_RS02150 at 67.823 kb on + strand, within SGL_RS02150 at 67.838 kb on + strand, within SGL_RS02150 at 67.850 kb on + strand, within SGL_RS02150 at 68.167 kb on + strand, within SGL_RS02150 at 68.528 kb on + strand, within SGL_RS02155 at 68.600 kb on + strand, within SGL_RS02155 at 68.720 kb on - strand, within SGL_RS02155 at 68.790 kb on - strand, within SGL_RS02155 at 68.879 kb on - strand, within SGL_RS02155 at 68.975 kb on - strand, within SGL_RS02155 at 68.988 kb on - strand, within SGL_RS02155 at 69.173 kb on + strand at 69.355 kb on - strand, within SGL_RS02160
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 66,671 - SGL_RS02145 0.49 +1.0 66,752 - SGL_RS02145 0.54 +0.8 66,757 - SGL_RS02145 0.54 -0.0 66,785 + SGL_RS02145 0.56 +0.7 66,785 + SGL_RS02145 0.56 -0.8 66,829 - SGL_RS02145 0.59 +0.2 66,835 + SGL_RS02145 0.59 +0.5 66,877 - SGL_RS02145 0.62 +0.3 66,972 - SGL_RS02145 0.67 +0.6 66,999 + SGL_RS02145 0.69 +1.9 67,043 - SGL_RS02145 0.71 -2.4 67,087 - SGL_RS02145 0.74 +0.1 67,163 - SGL_RS02145 0.78 +0.9 67,166 + SGL_RS02145 0.78 +1.5 67,200 - SGL_RS02145 0.80 -0.0 67,260 - SGL_RS02145 0.84 +1.7 67,322 + SGL_RS02145 0.88 -0.1 67,330 - SGL_RS02145 0.88 +0.5 67,330 - SGL_RS02145 0.88 +0.3 67,330 - SGL_RS02145 0.88 -0.0 67,399 + -0.4 67,399 + +2.3 67,406 + +1.0 67,463 - -0.3 67,657 + -0.2 67,660 - +1.0 67,786 + SGL_RS02150 0.24 -0.0 67,794 - SGL_RS02150 0.25 -0.7 67,823 + SGL_RS02150 0.29 -0.6 67,838 + SGL_RS02150 0.31 +0.1 67,850 + SGL_RS02150 0.32 -1.3 68,167 + SGL_RS02150 0.74 -1.1 68,528 + SGL_RS02155 0.19 +4.3 68,600 + SGL_RS02155 0.30 +4.6 68,720 - SGL_RS02155 0.48 +4.2 68,790 - SGL_RS02155 0.59 +5.0 68,879 - SGL_RS02155 0.73 +3.7 68,975 - SGL_RS02155 0.87 +4.6 68,988 - SGL_RS02155 0.89 +4.5 69,173 + -0.5 69,355 - SGL_RS02160 0.10 -0.4
Or see this region's nucleotide sequence