Experiment: R2A_PIPES with Cadmium chloride 128 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppx and ppk1 are separated by 81 nucleotides ppk1 and phoR are separated by 54 nucleotides
OKGIIK_03775: ppx - exopolyphosphatase, at 831,954 to 833,471
ppx
OKGIIK_03780: ppk1 - polyphosphate kinase 1, at 833,553 to 835,646
ppk1
OKGIIK_03785: phoR - phosphate regulon sensor histidine kinase PhoR, at 835,701 to 837,008
phoR
Position (kb) 833
834
835
836 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 832.822 kb on + strand, within ppx at 832.823 kb on - strand, within ppx at 832.823 kb on - strand, within ppx at 832.849 kb on + strand, within ppx at 832.850 kb on - strand, within ppx at 833.067 kb on + strand, within ppx at 833.067 kb on + strand, within ppx at 833.067 kb on + strand, within ppx at 833.068 kb on - strand, within ppx at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.518 kb on + strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.519 kb on - strand at 833.563 kb on + strand at 833.563 kb on + strand at 833.564 kb on - strand at 833.564 kb on - strand at 833.594 kb on - strand at 833.594 kb on - strand at 833.626 kb on + strand at 833.626 kb on + strand at 833.626 kb on + strand at 833.626 kb on + strand at 833.626 kb on + strand at 833.788 kb on + strand, within ppk1 at 833.788 kb on + strand, within ppk1 at 833.788 kb on + strand, within ppk1 at 833.789 kb on - strand, within ppk1 at 833.789 kb on - strand, within ppk1 at 833.789 kb on - strand, within ppk1 at 833.821 kb on + strand, within ppk1 at 833.821 kb on + strand, within ppk1 at 833.821 kb on + strand, within ppk1 at 833.821 kb on + strand, within ppk1 at 833.822 kb on - strand, within ppk1 at 833.822 kb on - strand, within ppk1 at 833.822 kb on - strand, within ppk1 at 833.822 kb on - strand, within ppk1 at 833.822 kb on - strand, within ppk1 at 833.822 kb on - strand, within ppk1 at 833.822 kb on - strand, within ppk1 at 833.959 kb on + strand, within ppk1 at 834.077 kb on - strand, within ppk1 at 834.077 kb on - strand, within ppk1 at 834.077 kb on - strand, within ppk1 at 834.077 kb on - strand, within ppk1 at 834.077 kb on - strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.205 kb on + strand, within ppk1 at 834.206 kb on - strand, within ppk1 at 834.206 kb on - strand, within ppk1 at 834.206 kb on - strand, within ppk1 at 834.206 kb on - strand, within ppk1 at 834.206 kb on - strand, within ppk1 at 834.206 kb on - strand, within ppk1 at 834.228 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.229 kb on + strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.230 kb on - strand, within ppk1 at 834.253 kb on + strand, within ppk1 at 834.253 kb on + strand, within ppk1 at 834.253 kb on + strand, within ppk1 at 834.254 kb on - strand, within ppk1 at 834.254 kb on - strand, within ppk1 at 834.254 kb on - strand, within ppk1 at 834.313 kb on + strand, within ppk1 at 834.313 kb on + strand, within ppk1 at 834.314 kb on - strand, within ppk1 at 834.328 kb on + strand, within ppk1 at 834.328 kb on + strand, within ppk1 at 834.328 kb on + strand, within ppk1 at 834.329 kb on - strand, within ppk1 at 834.329 kb on - strand, within ppk1 at 834.487 kb on + strand, within ppk1 at 834.488 kb on - strand, within ppk1 at 834.510 kb on + strand, within ppk1 at 834.510 kb on + strand, within ppk1 at 834.511 kb on - strand, within ppk1 at 834.511 kb on - strand, within ppk1 at 834.511 kb on - strand, within ppk1 at 834.511 kb on - strand, within ppk1 at 834.511 kb on - strand, within ppk1 at 834.511 kb on - strand, within ppk1 at 834.568 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.694 kb on + strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.695 kb on - strand, within ppk1 at 834.740 kb on - strand, within ppk1 at 834.740 kb on - strand, within ppk1 at 834.740 kb on - strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.222 kb on + strand, within ppk1 at 835.223 kb on - strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.327 kb on + strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.328 kb on - strand, within ppk1 at 835.421 kb on - strand, within ppk1 at 835.421 kb on - strand, within ppk1 at 835.421 kb on - strand, within ppk1 at 835.496 kb on - strand at 835.496 kb on - strand at 835.523 kb on + strand at 835.524 kb on - strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.588 kb on + strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.589 kb on - strand at 835.888 kb on + strand, within phoR at 835.889 kb on - strand, within phoR at 835.954 kb on + strand, within phoR at 835.954 kb on + strand, within phoR at 835.955 kb on - strand, within phoR at 836.014 kb on + strand, within phoR at 836.015 kb on - strand, within phoR at 836.326 kb on + strand, within phoR
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 128 uM remove 832,822 + ppx OKGIIK_03775 0.57 +1.2 832,823 - ppx OKGIIK_03775 0.57 -0.4 832,823 - ppx OKGIIK_03775 0.57 +0.6 832,849 + ppx OKGIIK_03775 0.59 -0.9 832,850 - ppx OKGIIK_03775 0.59 +1.4 833,067 + ppx OKGIIK_03775 0.73 +0.2 833,067 + ppx OKGIIK_03775 0.73 -1.4 833,067 + ppx OKGIIK_03775 0.73 +2.0 833,068 - ppx OKGIIK_03775 0.73 -1.9 833,518 + +0.5 833,518 + +0.8 833,518 + +0.3 833,518 + -0.7 833,518 + +0.8 833,518 + -0.1 833,518 + -1.6 833,518 + -0.3 833,518 + +0.2 833,518 + +1.2 833,518 + +0.5 833,518 + -1.4 833,518 + -0.6 833,518 + -0.3 833,518 + +0.2 833,519 - +0.0 833,519 - -0.4 833,519 - +2.2 833,519 - -0.5 833,519 - -1.2 833,519 - +0.3 833,519 - +0.6 833,519 - +0.2 833,519 - -2.0 833,519 - +1.7 833,519 - -0.4 833,519 - +0.9 833,519 - +1.2 833,519 - +1.2 833,519 - -2.2 833,519 - -2.0 833,519 - -1.0 833,519 - -1.0 833,519 - -0.2 833,563 + +0.3 833,563 + +0.2 833,564 - -0.2 833,564 - +0.0 833,594 - -0.6 833,594 - -0.1 833,626 + -0.4 833,626 + +1.5 833,626 + +0.5 833,626 + +0.4 833,626 + -0.6 833,788 + ppk1 OKGIIK_03780 0.11 +0.6 833,788 + ppk1 OKGIIK_03780 0.11 -0.6 833,788 + ppk1 OKGIIK_03780 0.11 -0.7 833,789 - ppk1 OKGIIK_03780 0.11 -0.3 833,789 - ppk1 OKGIIK_03780 0.11 +0.2 833,789 - ppk1 OKGIIK_03780 0.11 -0.1 833,821 + ppk1 OKGIIK_03780 0.13 +0.0 833,821 + ppk1 OKGIIK_03780 0.13 +1.2 833,821 + ppk1 OKGIIK_03780 0.13 +0.5 833,821 + ppk1 OKGIIK_03780 0.13 -1.5 833,822 - ppk1 OKGIIK_03780 0.13 -0.1 833,822 - ppk1 OKGIIK_03780 0.13 +1.2 833,822 - ppk1 OKGIIK_03780 0.13 -0.4 833,822 - ppk1 OKGIIK_03780 0.13 +1.1 833,822 - ppk1 OKGIIK_03780 0.13 +1.8 833,822 - ppk1 OKGIIK_03780 0.13 +0.7 833,822 - ppk1 OKGIIK_03780 0.13 +0.2 833,959 + ppk1 OKGIIK_03780 0.19 -1.2 834,077 - ppk1 OKGIIK_03780 0.25 -1.4 834,077 - ppk1 OKGIIK_03780 0.25 -0.1 834,077 - ppk1 OKGIIK_03780 0.25 +0.3 834,077 - ppk1 OKGIIK_03780 0.25 -1.0 834,077 - ppk1 OKGIIK_03780 0.25 -1.1 834,205 + ppk1 OKGIIK_03780 0.31 +0.6 834,205 + ppk1 OKGIIK_03780 0.31 -0.1 834,205 + ppk1 OKGIIK_03780 0.31 -1.0 834,205 + ppk1 OKGIIK_03780 0.31 -0.4 834,205 + ppk1 OKGIIK_03780 0.31 -0.8 834,205 + ppk1 OKGIIK_03780 0.31 +0.2 834,205 + ppk1 OKGIIK_03780 0.31 +1.3 834,205 + ppk1 OKGIIK_03780 0.31 -0.4 834,205 + ppk1 OKGIIK_03780 0.31 +0.2 834,205 + ppk1 OKGIIK_03780 0.31 -1.8 834,206 - ppk1 OKGIIK_03780 0.31 -1.0 834,206 - ppk1 OKGIIK_03780 0.31 +0.9 834,206 - ppk1 OKGIIK_03780 0.31 +1.1 834,206 - ppk1 OKGIIK_03780 0.31 +0.9 834,206 - ppk1 OKGIIK_03780 0.31 +0.1 834,206 - ppk1 OKGIIK_03780 0.31 -0.4 834,228 + ppk1 OKGIIK_03780 0.32 -0.1 834,229 + ppk1 OKGIIK_03780 0.32 +1.5 834,229 + ppk1 OKGIIK_03780 0.32 +0.5 834,229 + ppk1 OKGIIK_03780 0.32 -1.6 834,229 + ppk1 OKGIIK_03780 0.32 +0.6 834,229 + ppk1 OKGIIK_03780 0.32 +1.1 834,229 + ppk1 OKGIIK_03780 0.32 -1.4 834,229 + ppk1 OKGIIK_03780 0.32 -2.0 834,229 + ppk1 OKGIIK_03780 0.32 +0.2 834,229 + ppk1 OKGIIK_03780 0.32 -0.3 834,229 + ppk1 OKGIIK_03780 0.32 -1.2 834,229 + ppk1 OKGIIK_03780 0.32 -2.2 834,229 + ppk1 OKGIIK_03780 0.32 +1.5 834,229 + ppk1 OKGIIK_03780 0.32 -0.4 834,229 + ppk1 OKGIIK_03780 0.32 +0.9 834,229 + ppk1 OKGIIK_03780 0.32 +0.9 834,230 - ppk1 OKGIIK_03780 0.32 +0.5 834,230 - ppk1 OKGIIK_03780 0.32 +0.8 834,230 - ppk1 OKGIIK_03780 0.32 +1.2 834,230 - ppk1 OKGIIK_03780 0.32 -2.3 834,230 - ppk1 OKGIIK_03780 0.32 -2.2 834,230 - ppk1 OKGIIK_03780 0.32 -1.2 834,230 - ppk1 OKGIIK_03780 0.32 +0.2 834,230 - ppk1 OKGIIK_03780 0.32 +0.4 834,230 - ppk1 OKGIIK_03780 0.32 -2.9 834,230 - ppk1 OKGIIK_03780 0.32 -1.6 834,230 - ppk1 OKGIIK_03780 0.32 +0.2 834,230 - ppk1 OKGIIK_03780 0.32 -2.0 834,230 - ppk1 OKGIIK_03780 0.32 +0.2 834,253 + ppk1 OKGIIK_03780 0.33 +0.2 834,253 + ppk1 OKGIIK_03780 0.33 +0.2 834,253 + ppk1 OKGIIK_03780 0.33 +0.7 834,254 - ppk1 OKGIIK_03780 0.33 -0.6 834,254 - ppk1 OKGIIK_03780 0.33 -0.5 834,254 - ppk1 OKGIIK_03780 0.33 +0.2 834,313 + ppk1 OKGIIK_03780 0.36 +1.8 834,313 + ppk1 OKGIIK_03780 0.36 +0.0 834,314 - ppk1 OKGIIK_03780 0.36 -1.0 834,328 + ppk1 OKGIIK_03780 0.37 -1.9 834,328 + ppk1 OKGIIK_03780 0.37 -0.8 834,328 + ppk1 OKGIIK_03780 0.37 +0.6 834,329 - ppk1 OKGIIK_03780 0.37 -0.4 834,329 - ppk1 OKGIIK_03780 0.37 -0.6 834,487 + ppk1 OKGIIK_03780 0.45 -0.4 834,488 - ppk1 OKGIIK_03780 0.45 -0.0 834,510 + ppk1 OKGIIK_03780 0.46 -0.4 834,510 + ppk1 OKGIIK_03780 0.46 -0.4 834,511 - ppk1 OKGIIK_03780 0.46 -0.5 834,511 - ppk1 OKGIIK_03780 0.46 -0.1 834,511 - ppk1 OKGIIK_03780 0.46 +0.2 834,511 - ppk1 OKGIIK_03780 0.46 -0.2 834,511 - ppk1 OKGIIK_03780 0.46 +0.9 834,511 - ppk1 OKGIIK_03780 0.46 +1.2 834,568 + ppk1 OKGIIK_03780 0.48 +0.3 834,694 + ppk1 OKGIIK_03780 0.54 -1.2 834,694 + ppk1 OKGIIK_03780 0.54 -2.2 834,694 + ppk1 OKGIIK_03780 0.54 -1.2 834,694 + ppk1 OKGIIK_03780 0.54 -0.5 834,694 + ppk1 OKGIIK_03780 0.54 -0.6 834,694 + ppk1 OKGIIK_03780 0.54 +0.7 834,694 + ppk1 OKGIIK_03780 0.54 +0.2 834,694 + ppk1 OKGIIK_03780 0.54 -0.1 834,694 + ppk1 OKGIIK_03780 0.54 -0.1 834,695 - ppk1 OKGIIK_03780 0.55 -1.2 834,695 - ppk1 OKGIIK_03780 0.55 +2.5 834,695 - ppk1 OKGIIK_03780 0.55 -0.4 834,695 - ppk1 OKGIIK_03780 0.55 +0.4 834,695 - ppk1 OKGIIK_03780 0.55 -0.6 834,695 - ppk1 OKGIIK_03780 0.55 -0.4 834,695 - ppk1 OKGIIK_03780 0.55 +0.2 834,695 - ppk1 OKGIIK_03780 0.55 +0.2 834,695 - ppk1 OKGIIK_03780 0.55 -0.4 834,740 - ppk1 OKGIIK_03780 0.57 -0.5 834,740 - ppk1 OKGIIK_03780 0.57 -1.0 834,740 - ppk1 OKGIIK_03780 0.57 +0.4 835,222 + ppk1 OKGIIK_03780 0.80 -1.0 835,222 + ppk1 OKGIIK_03780 0.80 -0.4 835,222 + ppk1 OKGIIK_03780 0.80 -0.1 835,222 + ppk1 OKGIIK_03780 0.80 +0.2 835,223 - ppk1 OKGIIK_03780 0.80 +0.0 835,327 + ppk1 OKGIIK_03780 0.85 +0.1 835,327 + ppk1 OKGIIK_03780 0.85 -0.8 835,327 + ppk1 OKGIIK_03780 0.85 +0.3 835,327 + ppk1 OKGIIK_03780 0.85 +0.2 835,328 - ppk1 OKGIIK_03780 0.85 +0.0 835,328 - ppk1 OKGIIK_03780 0.85 +1.2 835,328 - ppk1 OKGIIK_03780 0.85 -0.1 835,328 - ppk1 OKGIIK_03780 0.85 -1.1 835,421 - ppk1 OKGIIK_03780 0.89 -1.0 835,421 - ppk1 OKGIIK_03780 0.89 -0.8 835,421 - ppk1 OKGIIK_03780 0.89 +1.0 835,496 - -0.1 835,496 - -0.8 835,523 + +0.3 835,524 - +0.6 835,588 + -0.0 835,588 + +0.8 835,588 + -1.0 835,588 + -1.1 835,588 + -1.6 835,588 + -0.3 835,588 + +0.5 835,589 - -1.4 835,589 - -2.4 835,589 - +1.5 835,589 - +0.3 835,589 - +0.6 835,589 - +0.3 835,589 - +0.2 835,589 - +0.2 835,888 + phoR OKGIIK_03785 0.14 +0.9 835,889 - phoR OKGIIK_03785 0.14 +0.2 835,954 + phoR OKGIIK_03785 0.19 +1.4 835,954 + phoR OKGIIK_03785 0.19 +0.2 835,955 - phoR OKGIIK_03785 0.19 -1.0 836,014 + phoR OKGIIK_03785 0.24 -1.4 836,015 - phoR OKGIIK_03785 0.24 -0.3 836,326 + phoR OKGIIK_03785 0.48 +0.6
Or see this region's nucleotide sequence