Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17300 and SGL_RS17305 are separated by 133 nucleotides SGL_RS17305 and SGL_RS17310 are separated by 14 nucleotides SGL_RS17310 and SGL_RS17315 are separated by 196 nucleotides SGL_RS17315 and SGL_RS17320 are separated by 36 nucleotides
SGL_RS17300: SGL_RS17300 - M3 family metallopeptidase, at 3,336,464 to 3,338,548
_RS17300
SGL_RS17305: SGL_RS17305 - pyrroline-5-carboxylate reductase, at 3,338,682 to 3,339,485
_RS17305
SGL_RS17310: SGL_RS17310 - glycoside hydrolase 100 family protein, at 3,339,500 to 3,340,969
_RS17310
SGL_RS17315: SGL_RS17315 - class I SAM-dependent methyltransferase, at 3,341,166 to 3,341,843
_RS17315
SGL_RS17320: SGL_RS17320 - hypothetical protein, at 3,341,880 to 3,342,128
_RS17320
Position (kb)
3339
3340
3341 Strain fitness (log2 ratio)
-1
0
1 at 3338.536 kb on - strand at 3338.538 kb on + strand at 3338.546 kb on - strand at 3338.668 kb on - strand at 3338.668 kb on - strand at 3338.859 kb on - strand, within SGL_RS17305 at 3338.912 kb on - strand, within SGL_RS17305 at 3339.007 kb on - strand, within SGL_RS17305 at 3339.032 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.137 kb on + strand, within SGL_RS17305 at 3339.255 kb on + strand, within SGL_RS17305 at 3339.367 kb on + strand, within SGL_RS17305 at 3339.553 kb on - strand at 3339.819 kb on - strand, within SGL_RS17310 at 3339.922 kb on - strand, within SGL_RS17310 at 3339.950 kb on + strand, within SGL_RS17310 at 3340.169 kb on - strand, within SGL_RS17310 at 3340.280 kb on + strand, within SGL_RS17310 at 3340.313 kb on + strand, within SGL_RS17310 at 3340.448 kb on + strand, within SGL_RS17310 at 3340.610 kb on + strand, within SGL_RS17310 at 3340.656 kb on + strand, within SGL_RS17310 at 3340.865 kb on + strand at 3340.990 kb on - strand at 3341.066 kb on - strand at 3341.275 kb on - strand, within SGL_RS17315 at 3341.482 kb on - strand, within SGL_RS17315 at 3341.482 kb on - strand, within SGL_RS17315 at 3341.506 kb on - strand, within SGL_RS17315 at 3341.525 kb on + strand, within SGL_RS17315 at 3341.586 kb on + strand, within SGL_RS17315 at 3341.655 kb on + strand, within SGL_RS17315 at 3341.768 kb on - strand, within SGL_RS17315
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 3,338,536 - +0.4 3,338,538 + +0.2 3,338,546 - -0.2 3,338,668 - -0.8 3,338,668 - -1.6 3,338,859 - SGL_RS17305 0.22 -1.3 3,338,912 - SGL_RS17305 0.29 -0.4 3,339,007 - SGL_RS17305 0.40 -1.3 3,339,032 + SGL_RS17305 0.44 -0.0 3,339,113 + SGL_RS17305 0.54 -0.4 3,339,113 + SGL_RS17305 0.54 -1.3 3,339,137 + SGL_RS17305 0.57 -1.4 3,339,255 + SGL_RS17305 0.71 -0.4 3,339,367 + SGL_RS17305 0.85 -0.8 3,339,553 - +0.7 3,339,819 - SGL_RS17310 0.22 +0.8 3,339,922 - SGL_RS17310 0.29 +1.1 3,339,950 + SGL_RS17310 0.31 +1.3 3,340,169 - SGL_RS17310 0.46 +0.7 3,340,280 + SGL_RS17310 0.53 +1.3 3,340,313 + SGL_RS17310 0.55 +0.5 3,340,448 + SGL_RS17310 0.64 +1.1 3,340,610 + SGL_RS17310 0.76 +0.6 3,340,656 + SGL_RS17310 0.79 +0.6 3,340,865 + +0.4 3,340,990 - +0.4 3,341,066 - -1.0 3,341,275 - SGL_RS17315 0.16 -0.5 3,341,482 - SGL_RS17315 0.47 +0.7 3,341,482 - SGL_RS17315 0.47 +0.3 3,341,506 - SGL_RS17315 0.50 -0.2 3,341,525 + SGL_RS17315 0.53 +0.1 3,341,586 + SGL_RS17315 0.62 +0.2 3,341,655 + SGL_RS17315 0.72 +0.9 3,341,768 - SGL_RS17315 0.89 +1.1
Or see this region's nucleotide sequence