Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16015 and SGL_RS19080 are separated by 22 nucleotides SGL_RS19080 and SGL_RS16020 are separated by 128 nucleotides SGL_RS16020 and SGL_RS16025 overlap by 20 nucleotides SGL_RS16025 and SGL_RS16030 are separated by 177 nucleotides
SGL_RS16015: SGL_RS16015 - type I methionyl aminopeptidase, at 3,042,789 to 3,043,613
_RS16015
SGL_RS19080: SGL_RS19080 - Rpn family recombination-promoting nuclease/putative transposase, at 3,043,636 to 3,043,926
_RS19080
SGL_RS16020: SGL_RS16020 - PIN domain-containing protein, at 3,044,055 to 3,044,438
_RS16020
SGL_RS16025: SGL_RS16025 - type II toxin-antitoxin system Phd/YefM family antitoxin, at 3,044,419 to 3,044,655
_RS16025
SGL_RS16030: SGL_RS16030 - bifunctional nicotinamide-nucleotide adenylyltransferase/Nudix hydroxylase, at 3,044,833 to 3,045,852
_RS16030
Position (kb)
3044
3045 Strain fitness (log2 ratio)
-1
0
1 at 3043.126 kb on + strand, within SGL_RS16015 at 3043.385 kb on + strand, within SGL_RS16015 at 3043.398 kb on + strand, within SGL_RS16015 at 3043.431 kb on + strand, within SGL_RS16015 at 3043.462 kb on + strand, within SGL_RS16015 at 3043.503 kb on - strand, within SGL_RS16015 at 3043.522 kb on + strand, within SGL_RS16015 at 3043.556 kb on + strand at 3043.575 kb on - strand at 3043.597 kb on + strand at 3043.838 kb on - strand, within SGL_RS19080 at 3043.941 kb on + strand at 3043.955 kb on - strand at 3043.985 kb on - strand at 3044.071 kb on + strand at 3044.123 kb on - strand, within SGL_RS16020 at 3044.138 kb on - strand, within SGL_RS16020 at 3044.177 kb on + strand, within SGL_RS16020 at 3044.180 kb on - strand, within SGL_RS16020 at 3044.228 kb on + strand, within SGL_RS16020 at 3044.236 kb on - strand, within SGL_RS16020 at 3044.241 kb on - strand, within SGL_RS16020 at 3044.330 kb on + strand, within SGL_RS16020 at 3044.331 kb on + strand, within SGL_RS16020 at 3044.339 kb on - strand, within SGL_RS16020 at 3044.339 kb on - strand, within SGL_RS16020 at 3044.344 kb on + strand, within SGL_RS16020 at 3044.344 kb on + strand, within SGL_RS16020 at 3044.396 kb on - strand, within SGL_RS16020 at 3044.434 kb on - strand at 3044.440 kb on + strand at 3044.459 kb on - strand, within SGL_RS16025 at 3044.485 kb on + strand, within SGL_RS16025 at 3044.498 kb on - strand, within SGL_RS16025 at 3044.662 kb on - strand at 3044.838 kb on - strand at 3044.879 kb on + strand at 3044.893 kb on + strand at 3044.903 kb on + strand at 3044.911 kb on - strand at 3044.911 kb on - strand at 3044.911 kb on - strand at 3044.993 kb on + strand, within SGL_RS16030 at 3045.032 kb on + strand, within SGL_RS16030 at 3045.162 kb on - strand, within SGL_RS16030 at 3045.356 kb on + strand, within SGL_RS16030 at 3045.392 kb on + strand, within SGL_RS16030 at 3045.392 kb on + strand, within SGL_RS16030 at 3045.402 kb on + strand, within SGL_RS16030 at 3045.402 kb on + strand, within SGL_RS16030 at 3045.402 kb on + strand, within SGL_RS16030 at 3045.410 kb on - strand, within SGL_RS16030
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 3,043,126 + SGL_RS16015 0.41 +0.2 3,043,385 + SGL_RS16015 0.72 -0.1 3,043,398 + SGL_RS16015 0.74 -0.3 3,043,431 + SGL_RS16015 0.78 -0.3 3,043,462 + SGL_RS16015 0.82 +0.0 3,043,503 - SGL_RS16015 0.87 +0.4 3,043,522 + SGL_RS16015 0.89 +0.3 3,043,556 + -0.3 3,043,575 - -0.9 3,043,597 + -0.1 3,043,838 - SGL_RS19080 0.69 +0.3 3,043,941 + -0.4 3,043,955 - -0.4 3,043,985 - -0.2 3,044,071 + -1.0 3,044,123 - SGL_RS16020 0.18 -0.5 3,044,138 - SGL_RS16020 0.22 -0.5 3,044,177 + SGL_RS16020 0.32 -0.8 3,044,180 - SGL_RS16020 0.33 -1.0 3,044,228 + SGL_RS16020 0.45 -0.1 3,044,236 - SGL_RS16020 0.47 -0.7 3,044,241 - SGL_RS16020 0.48 +0.1 3,044,330 + SGL_RS16020 0.72 -1.2 3,044,331 + SGL_RS16020 0.72 -0.4 3,044,339 - SGL_RS16020 0.74 +0.0 3,044,339 - SGL_RS16020 0.74 -0.0 3,044,344 + SGL_RS16020 0.75 -0.2 3,044,344 + SGL_RS16020 0.75 +0.3 3,044,396 - SGL_RS16020 0.89 +0.3 3,044,434 - +0.2 3,044,440 + +0.4 3,044,459 - SGL_RS16025 0.17 +0.2 3,044,485 + SGL_RS16025 0.28 +0.5 3,044,498 - SGL_RS16025 0.33 -0.6 3,044,662 - -0.4 3,044,838 - -0.3 3,044,879 + -0.8 3,044,893 + +0.3 3,044,903 + +0.4 3,044,911 - -0.2 3,044,911 - +0.1 3,044,911 - -0.1 3,044,993 + SGL_RS16030 0.16 +0.1 3,045,032 + SGL_RS16030 0.20 -0.2 3,045,162 - SGL_RS16030 0.32 +0.8 3,045,356 + SGL_RS16030 0.51 -0.1 3,045,392 + SGL_RS16030 0.55 +0.3 3,045,392 + SGL_RS16030 0.55 -0.2 3,045,402 + SGL_RS16030 0.56 -0.5 3,045,402 + SGL_RS16030 0.56 -0.6 3,045,402 + SGL_RS16030 0.56 -1.0 3,045,410 - SGL_RS16030 0.57 +1.5
Or see this region's nucleotide sequence