Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.3051.1 and Xcc-8004.3052.1 overlap by 4 nucleotides Xcc-8004.3052.1 and Xcc-8004.3053.1 are separated by 35 nucleotides Xcc-8004.3053.1 and Xcc-8004.3054.1 are separated by 3 nucleotides
Xcc-8004.3051.1: Xcc-8004.3051.1 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 2,984,375 to 2,985,229
Xcc-8004.3051.1
Xcc-8004.3052.1: Xcc-8004.3052.1 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 2,985,226 to 2,986,041
Xcc-8004.3052.1
Xcc-8004.3053.1: Xcc-8004.3053.1 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 2,986,077 to 2,986,562
Xcc-8004.3053.1
Xcc-8004.3054.1: Xcc-8004.3054.1 - Poly(A) polymerase (EC 2.7.7.19), at 2,986,566 to 2,987,933
Xcc-8004.3054.1
Position (kb)
2986
2987 Strain fitness (log2 ratio)
-1
0
1
2 at 2986.051 kb on - strand at 2986.051 kb on - strand at 2986.051 kb on - strand at 2986.051 kb on - strand at 2986.051 kb on - strand at 2986.063 kb on - strand at 2986.075 kb on - strand at 2986.075 kb on - strand at 2986.075 kb on - strand at 2986.075 kb on - strand at 2986.075 kb on - strand at 2986.075 kb on - strand at 2986.078 kb on + strand at 2987.459 kb on - strand, within Xcc-8004.3054.1 at 2987.459 kb on - strand, within Xcc-8004.3054.1 at 2987.459 kb on - strand, within Xcc-8004.3054.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together) remove 2,986,051 - +0.7 2,986,051 - -0.5 2,986,051 - -0.6 2,986,051 - +0.6 2,986,051 - +2.4 2,986,063 - +1.1 2,986,075 - +1.3 2,986,075 - -0.9 2,986,075 - -0.4 2,986,075 - +0.7 2,986,075 - +1.0 2,986,075 - +0.8 2,986,078 + -0.2 2,987,459 - Xcc-8004.3054.1 0.65 -1.6 2,987,459 - Xcc-8004.3054.1 0.65 +0.2 2,987,459 - Xcc-8004.3054.1 0.65 -0.5
Or see this region's nucleotide sequence