Experiment: 5-Aminovaleric carbon 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_04725 and PFR28_04726 are separated by 46 nucleotides PFR28_04726 and PFR28_04727 are separated by 275 nucleotides PFR28_04727 and PFR28_04728 are separated by 156 nucleotides
PFR28_04725: PFR28_04725 - NAD(P) transhydrogenase subunit alpha part 1, at 5,379,842 to 5,380,963
_04725
PFR28_04726: PFR28_04726 - hypothetical protein, at 5,381,010 to 5,381,105
_04726
PFR28_04727: PFR28_04727 - Glycine cleavage system transcriptional activator, at 5,381,381 to 5,382,295
_04727
PFR28_04728: PFR28_04728 - Acyl-CoA dehydrogenase, at 5,382,452 to 5,383,633
_04728
Position (kb)
5381
5382
5383 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5380.522 kb on - strand, within PFR28_04725 at 5380.522 kb on - strand, within PFR28_04725 at 5380.522 kb on - strand, within PFR28_04725 at 5380.522 kb on - strand, within PFR28_04725 at 5380.524 kb on + strand, within PFR28_04725 at 5380.524 kb on + strand, within PFR28_04725 at 5380.524 kb on + strand, within PFR28_04725 at 5380.525 kb on - strand, within PFR28_04725 at 5380.525 kb on - strand, within PFR28_04725 at 5380.525 kb on - strand, within PFR28_04725 at 5380.525 kb on - strand, within PFR28_04725 at 5380.525 kb on - strand, within PFR28_04725 at 5380.525 kb on - strand, within PFR28_04725 at 5380.551 kb on + strand, within PFR28_04725 at 5380.561 kb on + strand, within PFR28_04725 at 5380.690 kb on + strand, within PFR28_04725 at 5380.690 kb on + strand, within PFR28_04725 at 5380.691 kb on - strand, within PFR28_04725 at 5380.691 kb on - strand, within PFR28_04725 at 5380.815 kb on + strand, within PFR28_04725 at 5380.816 kb on - strand, within PFR28_04725 at 5380.870 kb on + strand at 5380.870 kb on + strand at 5380.870 kb on + strand at 5380.870 kb on + strand at 5380.871 kb on - strand at 5380.871 kb on - strand at 5380.871 kb on - strand at 5380.871 kb on - strand at 5380.871 kb on - strand at 5380.986 kb on - strand at 5380.986 kb on - strand at 5380.986 kb on - strand at 5380.992 kb on + strand at 5380.992 kb on + strand at 5380.992 kb on + strand at 5380.992 kb on + strand at 5380.993 kb on - strand at 5380.993 kb on - strand at 5381.125 kb on + strand at 5381.295 kb on + strand at 5381.295 kb on + strand at 5381.296 kb on - strand at 5381.325 kb on + strand at 5381.326 kb on - strand at 5381.343 kb on - strand at 5381.399 kb on + strand at 5381.400 kb on - strand at 5381.412 kb on + strand at 5381.493 kb on + strand, within PFR28_04727 at 5381.493 kb on + strand, within PFR28_04727 at 5381.493 kb on + strand, within PFR28_04727 at 5381.493 kb on + strand, within PFR28_04727 at 5381.493 kb on + strand, within PFR28_04727 at 5381.493 kb on + strand, within PFR28_04727 at 5381.493 kb on + strand, within PFR28_04727 at 5381.493 kb on + strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.494 kb on - strand, within PFR28_04727 at 5381.496 kb on + strand, within PFR28_04727 at 5381.496 kb on + strand, within PFR28_04727 at 5381.496 kb on + strand, within PFR28_04727 at 5381.497 kb on - strand, within PFR28_04727 at 5381.564 kb on + strand, within PFR28_04727 at 5381.564 kb on + strand, within PFR28_04727 at 5381.564 kb on + strand, within PFR28_04727 at 5381.564 kb on + strand, within PFR28_04727 at 5381.564 kb on + strand, within PFR28_04727 at 5381.565 kb on - strand, within PFR28_04727 at 5381.565 kb on - strand, within PFR28_04727 at 5381.565 kb on - strand, within PFR28_04727 at 5381.630 kb on + strand, within PFR28_04727 at 5381.630 kb on + strand, within PFR28_04727 at 5381.630 kb on + strand, within PFR28_04727 at 5381.630 kb on + strand, within PFR28_04727 at 5381.630 kb on + strand, within PFR28_04727 at 5381.631 kb on - strand, within PFR28_04727 at 5381.631 kb on - strand, within PFR28_04727 at 5381.631 kb on - strand, within PFR28_04727 at 5381.631 kb on - strand, within PFR28_04727 at 5381.631 kb on - strand, within PFR28_04727 at 5381.637 kb on + strand, within PFR28_04727 at 5381.704 kb on - strand, within PFR28_04727 at 5381.704 kb on - strand, within PFR28_04727 at 5381.859 kb on + strand, within PFR28_04727 at 5381.859 kb on + strand, within PFR28_04727 at 5381.859 kb on + strand, within PFR28_04727 at 5381.859 kb on + strand, within PFR28_04727 at 5381.859 kb on + strand, within PFR28_04727 at 5381.859 kb on + strand, within PFR28_04727 at 5381.859 kb on + strand, within PFR28_04727 at 5381.860 kb on - strand, within PFR28_04727 at 5381.860 kb on - strand, within PFR28_04727 at 5381.860 kb on - strand, within PFR28_04727 at 5381.860 kb on - strand, within PFR28_04727 at 5381.860 kb on - strand, within PFR28_04727 at 5381.860 kb on - strand, within PFR28_04727 at 5381.969 kb on + strand, within PFR28_04727 at 5381.969 kb on + strand, within PFR28_04727 at 5381.969 kb on + strand, within PFR28_04727 at 5381.996 kb on + strand, within PFR28_04727 at 5382.011 kb on + strand, within PFR28_04727 at 5382.011 kb on + strand, within PFR28_04727 at 5382.011 kb on + strand, within PFR28_04727 at 5382.011 kb on + strand, within PFR28_04727 at 5382.012 kb on - strand, within PFR28_04727 at 5382.089 kb on - strand, within PFR28_04727 at 5382.090 kb on + strand, within PFR28_04727 at 5382.090 kb on + strand, within PFR28_04727 at 5382.091 kb on - strand, within PFR28_04727 at 5382.097 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.185 kb on + strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.186 kb on - strand, within PFR28_04727 at 5382.199 kb on + strand, within PFR28_04727 at 5382.295 kb on + strand at 5382.296 kb on - strand at 5382.297 kb on + strand at 5382.297 kb on + strand at 5382.297 kb on + strand at 5382.297 kb on + strand at 5382.297 kb on + strand at 5382.297 kb on + strand at 5382.297 kb on + strand at 5382.297 kb on + strand at 5382.298 kb on - strand at 5382.298 kb on - strand at 5382.298 kb on - strand at 5382.298 kb on - strand at 5382.298 kb on - strand at 5382.298 kb on - strand at 5382.323 kb on + strand at 5382.324 kb on - strand at 5382.324 kb on - strand at 5382.324 kb on - strand at 5382.324 kb on - strand at 5382.324 kb on - strand at 5382.324 kb on - strand at 5382.324 kb on - strand at 5382.359 kb on - strand at 5382.408 kb on + strand at 5382.408 kb on + strand at 5382.408 kb on + strand at 5382.408 kb on + strand at 5382.409 kb on - strand at 5382.409 kb on - strand at 5382.668 kb on + strand, within PFR28_04728 at 5382.689 kb on + strand, within PFR28_04728 at 5382.689 kb on + strand, within PFR28_04728 at 5382.689 kb on + strand, within PFR28_04728 at 5382.689 kb on + strand, within PFR28_04728 at 5382.689 kb on + strand, within PFR28_04728 at 5382.690 kb on - strand, within PFR28_04728 at 5382.690 kb on - strand, within PFR28_04728 at 5382.699 kb on - strand, within PFR28_04728 at 5382.699 kb on - strand, within PFR28_04728 at 5382.699 kb on - strand, within PFR28_04728 at 5382.699 kb on - strand, within PFR28_04728 at 5382.699 kb on - strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.821 kb on + strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.822 kb on - strand, within PFR28_04728 at 5382.907 kb on + strand, within PFR28_04728 at 5382.908 kb on - strand, within PFR28_04728 at 5382.908 kb on - strand, within PFR28_04728 at 5382.908 kb on - strand, within PFR28_04728 at 5382.908 kb on - strand, within PFR28_04728 at 5382.936 kb on - strand, within PFR28_04728 at 5382.964 kb on + strand, within PFR28_04728 at 5382.964 kb on + strand, within PFR28_04728 at 5382.964 kb on + strand, within PFR28_04728 at 5382.965 kb on - strand, within PFR28_04728 at 5382.965 kb on - strand, within PFR28_04728 at 5382.987 kb on + strand, within PFR28_04728 at 5382.987 kb on + strand, within PFR28_04728 at 5382.987 kb on + strand, within PFR28_04728 at 5382.987 kb on + strand, within PFR28_04728 at 5382.988 kb on - strand, within PFR28_04728 at 5382.988 kb on - strand, within PFR28_04728 at 5382.988 kb on - strand, within PFR28_04728 at 5383.126 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.131 kb on + strand, within PFR28_04728 at 5383.132 kb on - strand, within PFR28_04728 at 5383.132 kb on - strand, within PFR28_04728 at 5383.132 kb on - strand, within PFR28_04728 at 5383.132 kb on - strand, within PFR28_04728 at 5383.132 kb on - strand, within PFR28_04728 at 5383.268 kb on + strand, within PFR28_04728
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Aminovaleric carbon 10 mM remove 5,380,522 - PFR28_04725 0.61 -1.2 5,380,522 - PFR28_04725 0.61 -0.0 5,380,522 - PFR28_04725 0.61 -0.1 5,380,522 - PFR28_04725 0.61 -0.1 5,380,524 + PFR28_04725 0.61 -1.2 5,380,524 + PFR28_04725 0.61 +0.1 5,380,524 + PFR28_04725 0.61 +2.6 5,380,525 - PFR28_04725 0.61 +0.0 5,380,525 - PFR28_04725 0.61 +0.0 5,380,525 - PFR28_04725 0.61 +0.2 5,380,525 - PFR28_04725 0.61 -1.3 5,380,525 - PFR28_04725 0.61 +0.4 5,380,525 - PFR28_04725 0.61 -0.3 5,380,551 + PFR28_04725 0.63 +0.2 5,380,561 + PFR28_04725 0.64 -0.3 5,380,690 + PFR28_04725 0.76 +0.2 5,380,690 + PFR28_04725 0.76 +0.1 5,380,691 - PFR28_04725 0.76 +0.4 5,380,691 - PFR28_04725 0.76 +0.3 5,380,815 + PFR28_04725 0.87 +0.3 5,380,816 - PFR28_04725 0.87 +1.0 5,380,870 + -0.7 5,380,870 + -0.0 5,380,870 + +0.5 5,380,870 + -1.2 5,380,871 - -0.7 5,380,871 - -1.9 5,380,871 - -2.4 5,380,871 - +0.2 5,380,871 - +0.3 5,380,986 - +0.5 5,380,986 - -0.8 5,380,986 - -0.3 5,380,992 + -1.0 5,380,992 + +1.5 5,380,992 + -2.3 5,380,992 + +0.5 5,380,993 - +1.8 5,380,993 - +0.7 5,381,125 + -0.1 5,381,295 + -0.4 5,381,295 + +0.2 5,381,296 - +0.6 5,381,325 + -0.3 5,381,326 - +1.2 5,381,343 - +0.2 5,381,399 + -2.4 5,381,400 - +0.4 5,381,412 + -0.4 5,381,493 + PFR28_04727 0.12 -1.7 5,381,493 + PFR28_04727 0.12 -4.2 5,381,493 + PFR28_04727 0.12 -3.1 5,381,493 + PFR28_04727 0.12 +0.0 5,381,493 + PFR28_04727 0.12 -0.4 5,381,493 + PFR28_04727 0.12 -2.7 5,381,493 + PFR28_04727 0.12 -3.5 5,381,493 + PFR28_04727 0.12 -4.2 5,381,494 - PFR28_04727 0.12 +0.6 5,381,494 - PFR28_04727 0.12 -2.1 5,381,494 - PFR28_04727 0.12 -3.8 5,381,494 - PFR28_04727 0.12 -3.5 5,381,494 - PFR28_04727 0.12 -1.7 5,381,494 - PFR28_04727 0.12 -2.8 5,381,494 - PFR28_04727 0.12 -1.0 5,381,494 - PFR28_04727 0.12 -4.0 5,381,494 - PFR28_04727 0.12 -2.3 5,381,496 + PFR28_04727 0.13 -1.6 5,381,496 + PFR28_04727 0.13 -4.2 5,381,496 + PFR28_04727 0.13 -2.9 5,381,497 - PFR28_04727 0.13 -2.0 5,381,564 + PFR28_04727 0.20 -2.3 5,381,564 + PFR28_04727 0.20 -1.9 5,381,564 + PFR28_04727 0.20 -4.1 5,381,564 + PFR28_04727 0.20 -2.9 5,381,564 + PFR28_04727 0.20 -4.7 5,381,565 - PFR28_04727 0.20 -1.5 5,381,565 - PFR28_04727 0.20 -2.0 5,381,565 - PFR28_04727 0.20 -2.4 5,381,630 + PFR28_04727 0.27 -3.6 5,381,630 + PFR28_04727 0.27 -1.9 5,381,630 + PFR28_04727 0.27 -2.2 5,381,630 + PFR28_04727 0.27 -3.5 5,381,630 + PFR28_04727 0.27 -1.6 5,381,631 - PFR28_04727 0.27 -3.8 5,381,631 - PFR28_04727 0.27 -1.6 5,381,631 - PFR28_04727 0.27 -4.0 5,381,631 - PFR28_04727 0.27 -3.5 5,381,631 - PFR28_04727 0.27 -3.8 5,381,637 + PFR28_04727 0.28 -4.0 5,381,704 - PFR28_04727 0.35 -3.4 5,381,704 - PFR28_04727 0.35 -3.5 5,381,859 + PFR28_04727 0.52 -3.6 5,381,859 + PFR28_04727 0.52 -4.8 5,381,859 + PFR28_04727 0.52 -1.9 5,381,859 + PFR28_04727 0.52 -5.7 5,381,859 + PFR28_04727 0.52 -3.2 5,381,859 + PFR28_04727 0.52 -3.3 5,381,859 + PFR28_04727 0.52 -2.9 5,381,860 - PFR28_04727 0.52 -3.5 5,381,860 - PFR28_04727 0.52 -4.3 5,381,860 - PFR28_04727 0.52 -3.2 5,381,860 - PFR28_04727 0.52 -3.5 5,381,860 - PFR28_04727 0.52 -2.2 5,381,860 - PFR28_04727 0.52 -3.9 5,381,969 + PFR28_04727 0.64 -0.2 5,381,969 + PFR28_04727 0.64 -1.6 5,381,969 + PFR28_04727 0.64 -2.0 5,381,996 + PFR28_04727 0.67 -1.0 5,382,011 + PFR28_04727 0.69 -3.3 5,382,011 + PFR28_04727 0.69 -2.4 5,382,011 + PFR28_04727 0.69 -1.4 5,382,011 + PFR28_04727 0.69 -1.9 5,382,012 - PFR28_04727 0.69 -4.2 5,382,089 - PFR28_04727 0.77 -1.2 5,382,090 + PFR28_04727 0.77 -2.7 5,382,090 + PFR28_04727 0.77 -3.9 5,382,091 - PFR28_04727 0.78 -3.9 5,382,097 + PFR28_04727 0.78 -4.7 5,382,185 + PFR28_04727 0.88 -2.5 5,382,185 + PFR28_04727 0.88 -2.3 5,382,185 + PFR28_04727 0.88 -2.1 5,382,185 + PFR28_04727 0.88 -3.4 5,382,185 + PFR28_04727 0.88 -4.0 5,382,185 + PFR28_04727 0.88 -3.2 5,382,185 + PFR28_04727 0.88 -4.0 5,382,185 + PFR28_04727 0.88 -2.9 5,382,185 + PFR28_04727 0.88 -4.0 5,382,185 + PFR28_04727 0.88 -4.3 5,382,186 - PFR28_04727 0.88 -2.7 5,382,186 - PFR28_04727 0.88 -2.2 5,382,186 - PFR28_04727 0.88 -3.9 5,382,186 - PFR28_04727 0.88 -4.4 5,382,186 - PFR28_04727 0.88 -2.7 5,382,186 - PFR28_04727 0.88 -3.7 5,382,186 - PFR28_04727 0.88 -3.7 5,382,186 - PFR28_04727 0.88 -4.4 5,382,199 + PFR28_04727 0.89 -3.9 5,382,295 + -3.7 5,382,296 - -0.7 5,382,297 + -3.7 5,382,297 + -3.8 5,382,297 + -1.6 5,382,297 + -1.7 5,382,297 + -2.5 5,382,297 + -2.8 5,382,297 + -3.6 5,382,297 + -4.3 5,382,298 - -0.2 5,382,298 - -1.9 5,382,298 - -0.2 5,382,298 - -0.9 5,382,298 - -0.5 5,382,298 - -1.9 5,382,323 + +1.6 5,382,324 - -2.3 5,382,324 - +0.7 5,382,324 - -0.0 5,382,324 - -0.1 5,382,324 - -0.2 5,382,324 - +0.5 5,382,324 - -1.1 5,382,359 - -3.2 5,382,408 + -2.2 5,382,408 + -3.0 5,382,408 + -2.9 5,382,408 + -2.5 5,382,409 - +0.0 5,382,409 - -3.6 5,382,668 + PFR28_04728 0.18 -3.4 5,382,689 + PFR28_04728 0.20 -4.1 5,382,689 + PFR28_04728 0.20 -1.6 5,382,689 + PFR28_04728 0.20 -3.6 5,382,689 + PFR28_04728 0.20 -2.5 5,382,689 + PFR28_04728 0.20 -3.2 5,382,690 - PFR28_04728 0.20 -2.9 5,382,690 - PFR28_04728 0.20 -2.5 5,382,699 - PFR28_04728 0.21 -2.5 5,382,699 - PFR28_04728 0.21 -3.9 5,382,699 - PFR28_04728 0.21 -1.4 5,382,699 - PFR28_04728 0.21 -1.6 5,382,699 - PFR28_04728 0.21 -0.7 5,382,821 + PFR28_04728 0.31 -3.4 5,382,821 + PFR28_04728 0.31 -3.9 5,382,821 + PFR28_04728 0.31 -2.2 5,382,821 + PFR28_04728 0.31 -1.2 5,382,821 + PFR28_04728 0.31 -1.6 5,382,821 + PFR28_04728 0.31 -3.2 5,382,821 + PFR28_04728 0.31 -1.0 5,382,821 + PFR28_04728 0.31 -0.4 5,382,821 + PFR28_04728 0.31 -2.5 5,382,822 - PFR28_04728 0.31 -2.9 5,382,822 - PFR28_04728 0.31 -2.2 5,382,822 - PFR28_04728 0.31 -2.7 5,382,822 - PFR28_04728 0.31 -2.5 5,382,822 - PFR28_04728 0.31 -4.6 5,382,822 - PFR28_04728 0.31 -4.2 5,382,822 - PFR28_04728 0.31 -3.9 5,382,822 - PFR28_04728 0.31 -3.1 5,382,822 - PFR28_04728 0.31 -3.3 5,382,907 + PFR28_04728 0.38 -2.0 5,382,908 - PFR28_04728 0.39 -2.9 5,382,908 - PFR28_04728 0.39 -2.8 5,382,908 - PFR28_04728 0.39 -3.4 5,382,908 - PFR28_04728 0.39 -3.6 5,382,936 - PFR28_04728 0.41 -3.3 5,382,964 + PFR28_04728 0.43 -3.3 5,382,964 + PFR28_04728 0.43 -2.8 5,382,964 + PFR28_04728 0.43 -3.0 5,382,965 - PFR28_04728 0.43 -2.9 5,382,965 - PFR28_04728 0.43 -2.0 5,382,987 + PFR28_04728 0.45 -3.6 5,382,987 + PFR28_04728 0.45 -1.6 5,382,987 + PFR28_04728 0.45 -2.6 5,382,987 + PFR28_04728 0.45 -0.7 5,382,988 - PFR28_04728 0.45 -2.5 5,382,988 - PFR28_04728 0.45 -3.6 5,382,988 - PFR28_04728 0.45 -4.0 5,383,126 + PFR28_04728 0.57 -1.2 5,383,131 + PFR28_04728 0.57 -2.4 5,383,131 + PFR28_04728 0.57 -2.6 5,383,131 + PFR28_04728 0.57 -1.5 5,383,131 + PFR28_04728 0.57 -3.2 5,383,131 + PFR28_04728 0.57 -2.4 5,383,131 + PFR28_04728 0.57 -1.9 5,383,131 + PFR28_04728 0.57 -2.7 5,383,131 + PFR28_04728 0.57 -2.6 5,383,131 + PFR28_04728 0.57 -2.1 5,383,132 - PFR28_04728 0.58 -3.4 5,383,132 - PFR28_04728 0.58 -4.0 5,383,132 - PFR28_04728 0.58 -2.8 5,383,132 - PFR28_04728 0.58 -3.4 5,383,132 - PFR28_04728 0.58 -4.4 5,383,268 + PFR28_04728 0.69 -2.2
Or see this region's nucleotide sequence