Experiment: LB_plus_SM_buffer with FelixO1_phage 0.05 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF313 and GFF314 are separated by 62 nucleotides GFF314 and GFF315 are separated by 9 nucleotides GFF315 and GFF316 are separated by 90 nucleotides GFF316 and GFF317 overlap by 17 nucleotides
GFF313 - Putative carbohydrate kinase in cluster with fructose-bisphosphate aldolase, at 30,841 to 32,325
GFF313
GFF314 - Fructose-bisphosphate aldolase (EC 4.1.2.13), at 32,388 to 33,248
GFF314
GFF315 - Phosphotransferase system HPr enzyme STM3779, at 33,258 to 33,527
GFF315
GFF316 - Putative DNA-binding protein in cluster with Type I restriction-modification system, at 33,618 to 33,941
GFF316
GFF317 - FIG074102: hypothetical protein, at 33,925 to 34,329
GFF317
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-2
-1
0
1 at 31.466 kb on + strand, within GFF313 at 31.535 kb on + strand, within GFF313 at 31.564 kb on - strand, within GFF313 at 31.638 kb on - strand, within GFF313 at 31.648 kb on - strand, within GFF313 at 32.406 kb on - strand at 32.799 kb on - strand, within GFF314 at 32.917 kb on + strand, within GFF314 at 32.917 kb on + strand, within GFF314 at 32.917 kb on + strand, within GFF314 at 32.959 kb on + strand, within GFF314 at 33.044 kb on + strand, within GFF314 at 33.055 kb on + strand, within GFF314 at 33.056 kb on - strand, within GFF314 at 33.255 kb on + strand at 33.281 kb on + strand at 33.524 kb on - strand at 33.634 kb on - strand at 33.656 kb on + strand, within GFF316 at 33.656 kb on + strand, within GFF316 at 33.657 kb on - strand, within GFF316 at 33.657 kb on - strand, within GFF316 at 33.657 kb on - strand, within GFF316 at 33.966 kb on + strand, within GFF317 at 33.967 kb on - strand, within GFF317 at 34.007 kb on + strand, within GFF317 at 34.018 kb on + strand, within GFF317 at 34.046 kb on + strand, within GFF317 at 34.050 kb on + strand, within GFF317 at 34.050 kb on + strand, within GFF317 at 34.050 kb on + strand, within GFF317 at 34.051 kb on - strand, within GFF317 at 34.051 kb on - strand, within GFF317 at 34.051 kb on - strand, within GFF317 at 34.176 kb on + strand, within GFF317 at 34.216 kb on + strand, within GFF317 at 34.216 kb on + strand, within GFF317
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.05 MOI remove 31,466 + GFF313 0.42 -0.8 31,535 + GFF313 0.47 +0.7 31,564 - GFF313 0.49 -0.7 31,638 - GFF313 0.54 -0.4 31,648 - GFF313 0.54 +0.2 32,406 - -0.8 32,799 - GFF314 0.48 -0.0 32,917 + GFF314 0.61 -0.3 32,917 + GFF314 0.61 +0.1 32,917 + GFF314 0.61 +0.8 32,959 + GFF314 0.66 -0.1 33,044 + GFF314 0.76 -2.2 33,055 + GFF314 0.77 +0.6 33,056 - GFF314 0.78 +0.2 33,255 + -0.4 33,281 + -0.0 33,524 - -0.8 33,634 - +0.4 33,656 + GFF316 0.12 +1.2 33,656 + GFF316 0.12 -0.0 33,657 - GFF316 0.12 -0.2 33,657 - GFF316 0.12 -0.2 33,657 - GFF316 0.12 -0.1 33,966 + GFF317 0.10 -0.6 33,967 - GFF317 0.10 +0.7 34,007 + GFF317 0.20 -0.3 34,018 + GFF317 0.23 -0.6 34,046 + GFF317 0.30 -0.6 34,050 + GFF317 0.31 +1.3 34,050 + GFF317 0.31 -0.2 34,050 + GFF317 0.31 +0.4 34,051 - GFF317 0.31 +0.2 34,051 - GFF317 0.31 +0.1 34,051 - GFF317 0.31 -0.2 34,176 + GFF317 0.62 -0.0 34,216 + GFF317 0.72 +0.7 34,216 + GFF317 0.72 +0.7
Or see this region's nucleotide sequence