Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt stpA and ygaW are separated by 668 nucleotides ygaW and ygaC are separated by 36 nucleotides ygaC and ygaM are separated by 139 nucleotides
b2669: stpA - DNA binding protein, nucleoid-associated (NCBI), at 2,796,113 to 2,796,517
stpA
b2670: ygaW - predicted inner membrane protein (NCBI), at 2,797,186 to 2,797,635
ygaW
b2671: ygaC - hypothetical protein (NCBI), at 2,797,672 to 2,798,016
ygaC
b2672: ygaM - orf, hypothetical protein (VIMSS), at 2,798,156 to 2,798,497
ygaM
Position (kb)
2797
2798 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2796.189 kb on + strand, within stpA at 2796.197 kb on - strand, within stpA at 2796.197 kb on - strand, within stpA at 2796.201 kb on - strand, within stpA at 2796.202 kb on - strand, within stpA at 2796.203 kb on - strand, within stpA at 2796.215 kb on - strand, within stpA at 2796.215 kb on - strand, within stpA at 2796.215 kb on - strand, within stpA at 2796.266 kb on + strand, within stpA at 2796.271 kb on - strand, within stpA at 2796.271 kb on - strand, within stpA at 2796.271 kb on - strand, within stpA at 2796.276 kb on - strand, within stpA at 2796.276 kb on - strand, within stpA at 2796.276 kb on - strand, within stpA at 2796.279 kb on + strand, within stpA at 2796.281 kb on - strand, within stpA at 2796.281 kb on - strand, within stpA at 2796.290 kb on - strand, within stpA at 2796.290 kb on - strand, within stpA at 2796.326 kb on - strand, within stpA at 2796.326 kb on - strand, within stpA at 2796.326 kb on - strand, within stpA at 2796.326 kb on - strand, within stpA at 2796.326 kb on - strand, within stpA at 2796.329 kb on - strand, within stpA at 2796.331 kb on + strand, within stpA at 2796.336 kb on - strand, within stpA at 2796.336 kb on - strand, within stpA at 2796.336 kb on - strand, within stpA at 2796.336 kb on - strand, within stpA at 2796.339 kb on - strand, within stpA at 2796.364 kb on - strand, within stpA at 2796.364 kb on - strand, within stpA at 2796.365 kb on - strand, within stpA at 2796.365 kb on - strand, within stpA at 2796.369 kb on - strand, within stpA at 2796.374 kb on - strand, within stpA at 2796.375 kb on - strand, within stpA at 2796.375 kb on - strand, within stpA at 2796.376 kb on + strand, within stpA at 2796.385 kb on - strand, within stpA at 2796.394 kb on - strand, within stpA at 2796.406 kb on - strand, within stpA at 2796.407 kb on + strand, within stpA at 2796.407 kb on + strand, within stpA at 2796.442 kb on - strand, within stpA at 2796.443 kb on + strand, within stpA at 2796.452 kb on - strand, within stpA at 2796.452 kb on - strand, within stpA at 2796.474 kb on - strand, within stpA at 2796.488 kb on - strand at 2796.488 kb on - strand at 2796.576 kb on - strand at 2796.619 kb on - strand at 2796.709 kb on - strand at 2796.709 kb on - strand at 2796.718 kb on - strand at 2796.718 kb on - strand at 2796.718 kb on - strand at 2796.728 kb on - strand at 2796.733 kb on - strand at 2796.742 kb on - strand at 2796.742 kb on - strand at 2796.742 kb on - strand at 2796.882 kb on - strand at 2796.891 kb on + strand at 2797.102 kb on - strand at 2797.199 kb on + strand at 2797.199 kb on + strand at 2797.240 kb on + strand, within ygaW at 2797.303 kb on - strand, within ygaW at 2797.303 kb on - strand, within ygaW at 2797.323 kb on + strand, within ygaW at 2797.323 kb on + strand, within ygaW at 2797.362 kb on + strand, within ygaW at 2797.362 kb on + strand, within ygaW at 2797.364 kb on + strand, within ygaW at 2797.364 kb on + strand, within ygaW at 2797.364 kb on - strand, within ygaW at 2797.369 kb on - strand, within ygaW at 2797.369 kb on - strand, within ygaW at 2797.482 kb on + strand, within ygaW at 2797.482 kb on + strand, within ygaW at 2797.484 kb on + strand, within ygaW at 2797.492 kb on - strand, within ygaW at 2797.512 kb on + strand, within ygaW at 2797.512 kb on + strand, within ygaW at 2797.607 kb on - strand at 2797.607 kb on - strand at 2797.612 kb on - strand at 2797.612 kb on - strand at 2797.613 kb on - strand at 2797.733 kb on - strand, within ygaC at 2797.733 kb on - strand, within ygaC at 2797.739 kb on - strand, within ygaC at 2797.777 kb on - strand, within ygaC at 2797.841 kb on - strand, within ygaC at 2797.841 kb on - strand, within ygaC at 2797.872 kb on + strand, within ygaC at 2797.879 kb on - strand, within ygaC at 2797.882 kb on + strand, within ygaC at 2797.888 kb on - strand, within ygaC at 2797.888 kb on - strand, within ygaC at 2797.888 kb on - strand, within ygaC at 2797.935 kb on - strand, within ygaC at 2797.941 kb on + strand, within ygaC at 2798.044 kb on + strand at 2798.044 kb on + strand at 2798.048 kb on + strand at 2798.049 kb on + strand at 2798.049 kb on + strand at 2798.216 kb on + strand, within ygaM at 2798.340 kb on + strand, within ygaM at 2798.387 kb on + strand, within ygaM at 2798.395 kb on - strand, within ygaM at 2798.427 kb on + strand, within ygaM at 2798.481 kb on - strand at 2798.481 kb on - strand at 2798.600 kb on + strand at 2798.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,796,189 + stpA b2669 0.19 +0.6 2,796,197 - stpA b2669 0.21 -0.8 2,796,197 - stpA b2669 0.21 -0.2 2,796,201 - stpA b2669 0.22 -0.6 2,796,202 - stpA b2669 0.22 -0.0 2,796,203 - stpA b2669 0.22 -0.6 2,796,215 - stpA b2669 0.25 -0.1 2,796,215 - stpA b2669 0.25 -1.4 2,796,215 - stpA b2669 0.25 +0.5 2,796,266 + stpA b2669 0.38 -0.3 2,796,271 - stpA b2669 0.39 -0.0 2,796,271 - stpA b2669 0.39 +0.4 2,796,271 - stpA b2669 0.39 -1.2 2,796,276 - stpA b2669 0.40 +0.4 2,796,276 - stpA b2669 0.40 +0.4 2,796,276 - stpA b2669 0.40 -0.1 2,796,279 + stpA b2669 0.41 -0.7 2,796,281 - stpA b2669 0.41 -0.8 2,796,281 - stpA b2669 0.41 +0.4 2,796,290 - stpA b2669 0.44 -0.2 2,796,290 - stpA b2669 0.44 -0.4 2,796,326 - stpA b2669 0.53 +0.0 2,796,326 - stpA b2669 0.53 +0.2 2,796,326 - stpA b2669 0.53 -0.6 2,796,326 - stpA b2669 0.53 -1.4 2,796,326 - stpA b2669 0.53 +0.3 2,796,329 - stpA b2669 0.53 +0.1 2,796,331 + stpA b2669 0.54 +0.6 2,796,336 - stpA b2669 0.55 -1.0 2,796,336 - stpA b2669 0.55 +0.9 2,796,336 - stpA b2669 0.55 -0.9 2,796,336 - stpA b2669 0.55 -1.4 2,796,339 - stpA b2669 0.56 +0.2 2,796,364 - stpA b2669 0.62 +0.6 2,796,364 - stpA b2669 0.62 +0.3 2,796,365 - stpA b2669 0.62 +0.2 2,796,365 - stpA b2669 0.62 -0.3 2,796,369 - stpA b2669 0.63 +0.2 2,796,374 - stpA b2669 0.64 +0.2 2,796,375 - stpA b2669 0.65 +0.6 2,796,375 - stpA b2669 0.65 -1.0 2,796,376 + stpA b2669 0.65 +0.2 2,796,385 - stpA b2669 0.67 +0.2 2,796,394 - stpA b2669 0.69 -0.8 2,796,406 - stpA b2669 0.72 +0.2 2,796,407 + stpA b2669 0.73 +0.6 2,796,407 + stpA b2669 0.73 -0.5 2,796,442 - stpA b2669 0.81 +0.1 2,796,443 + stpA b2669 0.81 -0.9 2,796,452 - stpA b2669 0.84 +0.7 2,796,452 - stpA b2669 0.84 +0.5 2,796,474 - stpA b2669 0.89 +0.3 2,796,488 - -0.2 2,796,488 - +1.0 2,796,576 - +0.7 2,796,619 - +0.2 2,796,709 - +0.2 2,796,709 - +0.3 2,796,718 - -0.9 2,796,718 - +0.5 2,796,718 - +0.1 2,796,728 - +0.1 2,796,733 - -0.4 2,796,742 - +0.3 2,796,742 - +0.4 2,796,742 - -0.0 2,796,882 - +0.3 2,796,891 + +0.2 2,797,102 - -1.9 2,797,199 + -0.7 2,797,199 + -3.0 2,797,240 + ygaW b2670 0.12 -0.4 2,797,303 - ygaW b2670 0.26 -1.1 2,797,303 - ygaW b2670 0.26 -1.4 2,797,323 + ygaW b2670 0.30 -0.7 2,797,323 + ygaW b2670 0.30 -0.8 2,797,362 + ygaW b2670 0.39 -2.4 2,797,362 + ygaW b2670 0.39 -2.1 2,797,364 + ygaW b2670 0.40 -0.8 2,797,364 + ygaW b2670 0.40 -2.3 2,797,364 - ygaW b2670 0.40 -1.9 2,797,369 - ygaW b2670 0.41 -1.8 2,797,369 - ygaW b2670 0.41 -0.9 2,797,482 + ygaW b2670 0.66 -2.1 2,797,482 + ygaW b2670 0.66 +0.3 2,797,484 + ygaW b2670 0.66 -1.6 2,797,492 - ygaW b2670 0.68 -1.5 2,797,512 + ygaW b2670 0.72 -3.5 2,797,512 + ygaW b2670 0.72 -1.4 2,797,607 - -0.6 2,797,607 - -1.7 2,797,612 - -2.8 2,797,612 - +0.9 2,797,613 - -1.3 2,797,733 - ygaC b2671 0.18 -0.6 2,797,733 - ygaC b2671 0.18 +2.0 2,797,739 - ygaC b2671 0.19 +0.5 2,797,777 - ygaC b2671 0.30 -0.9 2,797,841 - ygaC b2671 0.49 -0.1 2,797,841 - ygaC b2671 0.49 -0.4 2,797,872 + ygaC b2671 0.58 +0.3 2,797,879 - ygaC b2671 0.60 -1.3 2,797,882 + ygaC b2671 0.61 +0.0 2,797,888 - ygaC b2671 0.63 +0.2 2,797,888 - ygaC b2671 0.63 -1.1 2,797,888 - ygaC b2671 0.63 -0.2 2,797,935 - ygaC b2671 0.76 +0.3 2,797,941 + ygaC b2671 0.78 -1.0 2,798,044 + +0.3 2,798,044 + -0.4 2,798,048 + -1.5 2,798,049 + +0.6 2,798,049 + -0.4 2,798,216 + ygaM b2672 0.18 -0.5 2,798,340 + ygaM b2672 0.54 -0.1 2,798,387 + ygaM b2672 0.68 +0.1 2,798,395 - ygaM b2672 0.70 -0.7 2,798,427 + ygaM b2672 0.79 -1.0 2,798,481 - -0.1 2,798,481 - +0.2 2,798,600 + -0.1 2,798,614 - -0.6
Or see this region's nucleotide sequence