Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF760 and GFF761 overlap by 4 nucleotides GFF761 and GFF762 overlap by 17 nucleotides GFF762 and GFF763 are separated by 42 nucleotides
GFF760 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 117,792 to 118,745
GFF760
GFF761 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 118,742 to 119,587
GFF761
GFF762 - Putative inner membrane protein, at 119,571 to 120,134
GFF762
GFF763 - 23S rRNA (Uracil-5-) -methyltransferase rumB (EC 2.1.1.-), at 120,177 to 121,307
GFF763
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 118.593 kb on - strand, within GFF760 at 118.668 kb on - strand at 118.670 kb on + strand at 118.670 kb on + strand at 118.671 kb on - strand at 118.671 kb on - strand at 118.687 kb on + strand at 118.688 kb on - strand at 118.710 kb on - strand at 118.747 kb on + strand at 118.748 kb on - strand at 118.802 kb on - strand at 118.895 kb on + strand, within GFF761 at 119.230 kb on - strand, within GFF761 at 119.671 kb on + strand, within GFF762 at 119.792 kb on - strand, within GFF762 at 119.915 kb on + strand, within GFF762 at 119.916 kb on - strand, within GFF762 at 119.935 kb on - strand, within GFF762 at 119.951 kb on + strand, within GFF762 at 120.076 kb on + strand, within GFF762 at 120.132 kb on + strand at 120.139 kb on + strand at 120.140 kb on - strand at 120.176 kb on + strand at 120.176 kb on + strand at 120.177 kb on - strand at 120.271 kb on + strand at 120.372 kb on - strand, within GFF763 at 120.513 kb on + strand, within GFF763 at 120.514 kb on - strand, within GFF763 at 120.514 kb on - strand, within GFF763 at 120.516 kb on - strand, within GFF763 at 120.590 kb on - strand, within GFF763 at 120.689 kb on + strand, within GFF763 at 120.704 kb on + strand, within GFF763 at 120.821 kb on + strand, within GFF763 at 120.838 kb on + strand, within GFF763 at 120.839 kb on - strand, within GFF763 at 121.093 kb on + strand, within GFF763
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 118,593 - GFF760 0.84 -0.3 118,668 - +0.6 118,670 + -0.2 118,670 + -0.5 118,671 - -1.2 118,671 - +0.3 118,687 + -0.1 118,688 - +0.1 118,710 - +0.5 118,747 + -0.6 118,748 - +0.1 118,802 - +0.8 118,895 + GFF761 0.18 +0.5 119,230 - GFF761 0.58 +0.6 119,671 + GFF762 0.18 -0.0 119,792 - GFF762 0.39 -2.0 119,915 + GFF762 0.61 +0.6 119,916 - GFF762 0.61 -1.8 119,935 - GFF762 0.65 -0.7 119,951 + GFF762 0.67 -2.0 120,076 + GFF762 0.90 -0.3 120,132 + -0.9 120,139 + -1.2 120,140 - +0.7 120,176 + -0.8 120,176 + +1.3 120,177 - +1.7 120,271 + -0.8 120,372 - GFF763 0.17 -0.6 120,513 + GFF763 0.30 -0.7 120,514 - GFF763 0.30 +0.7 120,514 - GFF763 0.30 +0.9 120,516 - GFF763 0.30 +2.2 120,590 - GFF763 0.37 +0.5 120,689 + GFF763 0.45 -1.2 120,704 + GFF763 0.47 -0.6 120,821 + GFF763 0.57 -0.2 120,838 + GFF763 0.58 -1.7 120,839 - GFF763 0.59 -0.6 121,093 + GFF763 0.81 -1.4
Or see this region's nucleotide sequence