Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15155 and EX31_RS15160 overlap by 4 nucleotides EX31_RS15160 and EX31_RS15165 overlap by 4 nucleotides EX31_RS15165 and EX31_RS15170 overlap by 1 nucleotides EX31_RS15170 and EX31_RS15175 are separated by 96 nucleotides
EX31_RS15155: EX31_RS15155 - diaminopimelate epimerase, at 48,323 to 49,147
_RS15155
EX31_RS15160: EX31_RS15160 - DUF484 domain-containing protein, at 49,144 to 49,848
_RS15160
EX31_RS15165: EX31_RS15165 - tyrosine recombinase XerC, at 49,845 to 50,756
_RS15165
EX31_RS15170: EX31_RS15170 - 5-amino-6-(5-phospho-D-ribitylamino)uracil phosphatase YigB, at 50,756 to 51,472
_RS15170
EX31_RS15175: EX31_RS15175 - DNA helicase II, at 51,569 to 53,731
_RS15175
Position (kb) 49
50
51 Strain fitness (log2 ratio) -2
-1
0
1
2 at 48.927 kb on + strand, within EX31_RS15155 at 49.097 kb on + strand at 49.099 kb on + strand at 49.099 kb on + strand at 49.135 kb on + strand at 49.135 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.143 kb on + strand at 49.144 kb on - strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.178 kb on + strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.179 kb on - strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.356 kb on + strand, within EX31_RS15160 at 49.356 kb on + strand, within EX31_RS15160 at 49.356 kb on + strand, within EX31_RS15160 at 49.356 kb on + strand, within EX31_RS15160 at 49.357 kb on - strand, within EX31_RS15160 at 49.452 kb on + strand, within EX31_RS15160 at 49.652 kb on + strand, within EX31_RS15160 at 49.653 kb on - strand, within EX31_RS15160 at 49.661 kb on + strand, within EX31_RS15160 at 49.662 kb on - strand, within EX31_RS15160 at 49.787 kb on + strand at 49.844 kb on + strand at 49.897 kb on - strand at 49.929 kb on - strand at 49.941 kb on + strand, within EX31_RS15165 at 49.941 kb on + strand, within EX31_RS15165 at 49.941 kb on + strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.942 kb on - strand, within EX31_RS15165 at 49.954 kb on + strand, within EX31_RS15165 at 49.955 kb on - strand, within EX31_RS15165 at 49.988 kb on + strand, within EX31_RS15165 at 50.046 kb on - strand, within EX31_RS15165 at 50.217 kb on - strand, within EX31_RS15165 at 50.283 kb on + strand, within EX31_RS15165 at 50.283 kb on + strand, within EX31_RS15165 at 50.284 kb on - strand, within EX31_RS15165 at 50.284 kb on - strand, within EX31_RS15165 at 50.284 kb on - strand, within EX31_RS15165 at 50.333 kb on + strand, within EX31_RS15165 at 50.333 kb on + strand, within EX31_RS15165 at 50.334 kb on - strand, within EX31_RS15165 at 50.499 kb on - strand, within EX31_RS15165 at 50.565 kb on - strand, within EX31_RS15165 at 50.679 kb on + strand at 50.679 kb on + strand at 50.679 kb on + strand at 50.680 kb on - strand at 50.766 kb on - strand at 50.805 kb on + strand at 50.819 kb on + strand at 50.819 kb on + strand at 50.820 kb on - strand at 50.820 kb on - strand at 50.828 kb on + strand, within EX31_RS15170 at 50.828 kb on + strand, within EX31_RS15170 at 50.829 kb on - strand, within EX31_RS15170 at 50.829 kb on - strand, within EX31_RS15170 at 50.829 kb on - strand, within EX31_RS15170 at 50.961 kb on - strand, within EX31_RS15170 at 51.092 kb on + strand, within EX31_RS15170 at 51.092 kb on + strand, within EX31_RS15170 at 51.092 kb on + strand, within EX31_RS15170 at 51.093 kb on - strand, within EX31_RS15170 at 51.093 kb on - strand, within EX31_RS15170 at 51.093 kb on - strand, within EX31_RS15170 at 51.093 kb on - strand, within EX31_RS15170 at 51.093 kb on - strand, within EX31_RS15170 at 51.093 kb on - strand, within EX31_RS15170 at 51.154 kb on + strand, within EX31_RS15170 at 51.154 kb on + strand, within EX31_RS15170 at 51.154 kb on + strand, within EX31_RS15170 at 51.155 kb on - strand, within EX31_RS15170 at 51.200 kb on + strand, within EX31_RS15170 at 51.200 kb on + strand, within EX31_RS15170 at 51.200 kb on + strand, within EX31_RS15170 at 51.201 kb on - strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.211 kb on + strand, within EX31_RS15170 at 51.212 kb on - strand, within EX31_RS15170 at 51.212 kb on - strand, within EX31_RS15170 at 51.248 kb on + strand, within EX31_RS15170 at 51.260 kb on + strand, within EX31_RS15170 at 51.260 kb on + strand, within EX31_RS15170 at 51.260 kb on + strand, within EX31_RS15170 at 51.260 kb on + strand, within EX31_RS15170 at 51.260 kb on + strand, within EX31_RS15170 at 51.261 kb on - strand, within EX31_RS15170 at 51.261 kb on - strand, within EX31_RS15170 at 51.261 kb on - strand, within EX31_RS15170 at 51.261 kb on - strand, within EX31_RS15170 at 51.261 kb on - strand, within EX31_RS15170 at 51.263 kb on - strand, within EX31_RS15170 at 51.263 kb on - strand, within EX31_RS15170 at 51.377 kb on - strand, within EX31_RS15170 at 51.431 kb on - strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.468 kb on + strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.469 kb on - strand at 51.471 kb on - strand at 51.481 kb on + strand at 51.495 kb on + strand at 51.568 kb on + strand at 51.569 kb on - strand at 51.569 kb on - strand at 51.732 kb on - strand at 51.736 kb on + strand at 51.736 kb on + strand at 51.736 kb on + strand at 51.736 kb on + strand at 51.736 kb on + strand at 51.736 kb on + strand at 51.737 kb on - strand at 51.737 kb on - strand at 51.737 kb on - strand at 51.737 kb on - strand at 51.737 kb on - strand at 51.739 kb on + strand at 51.739 kb on + strand at 51.740 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 48,927 + EX31_RS15155 0.73 -0.1 49,097 + -0.6 49,099 + -0.6 49,099 + -1.7 49,135 + -1.2 49,135 + -0.1 49,143 + +0.2 49,143 + -0.2 49,143 + -0.5 49,143 + +0.4 49,143 + +0.4 49,143 + -0.2 49,143 + -0.5 49,143 + -0.8 49,143 + +0.6 49,143 + -0.0 49,143 + +0.7 49,143 + -1.4 49,144 - -1.6 49,178 + -0.3 49,178 + +0.1 49,178 + -0.9 49,178 + -0.3 49,178 + -0.1 49,178 + -0.5 49,178 + +0.0 49,178 + -0.6 49,178 + -0.0 49,178 + +0.9 49,179 - -2.0 49,179 - +0.4 49,179 - -0.7 49,179 - -2.0 49,179 - -2.6 49,179 - -1.3 49,207 + +0.8 49,207 + -0.4 49,207 + +0.7 49,207 + +0.1 49,207 + +0.1 49,356 + EX31_RS15160 0.30 -0.7 49,356 + EX31_RS15160 0.30 -1.1 49,356 + EX31_RS15160 0.30 +0.0 49,356 + EX31_RS15160 0.30 -0.1 49,357 - EX31_RS15160 0.30 -1.4 49,452 + EX31_RS15160 0.44 +0.1 49,652 + EX31_RS15160 0.72 -0.5 49,653 - EX31_RS15160 0.72 -0.4 49,661 + EX31_RS15160 0.73 -1.0 49,662 - EX31_RS15160 0.73 +0.4 49,787 + -0.0 49,844 + -0.5 49,897 - +1.2 49,929 - -0.1 49,941 + EX31_RS15165 0.11 -0.6 49,941 + EX31_RS15165 0.11 -1.0 49,941 + EX31_RS15165 0.11 -1.4 49,942 - EX31_RS15165 0.11 +0.6 49,942 - EX31_RS15165 0.11 +0.4 49,942 - EX31_RS15165 0.11 +0.4 49,942 - EX31_RS15165 0.11 -0.5 49,954 + EX31_RS15165 0.12 -1.6 49,955 - EX31_RS15165 0.12 +0.8 49,988 + EX31_RS15165 0.16 -1.6 50,046 - EX31_RS15165 0.22 -1.0 50,217 - EX31_RS15165 0.41 -0.6 50,283 + EX31_RS15165 0.48 -1.8 50,283 + EX31_RS15165 0.48 -1.1 50,284 - EX31_RS15165 0.48 -2.0 50,284 - EX31_RS15165 0.48 -1.6 50,284 - EX31_RS15165 0.48 -1.3 50,333 + EX31_RS15165 0.54 -0.3 50,333 + EX31_RS15165 0.54 +0.6 50,334 - EX31_RS15165 0.54 -0.7 50,499 - EX31_RS15165 0.72 +0.0 50,565 - EX31_RS15165 0.79 -1.5 50,679 + +0.4 50,679 + +0.1 50,679 + -1.0 50,680 - -0.4 50,766 - +0.6 50,805 + -0.1 50,819 + +0.5 50,819 + -0.7 50,820 - -0.3 50,820 - -0.9 50,828 + EX31_RS15170 0.10 +0.6 50,828 + EX31_RS15170 0.10 +0.1 50,829 - EX31_RS15170 0.10 -0.5 50,829 - EX31_RS15170 0.10 +0.6 50,829 - EX31_RS15170 0.10 +0.9 50,961 - EX31_RS15170 0.29 +1.2 51,092 + EX31_RS15170 0.47 +0.4 51,092 + EX31_RS15170 0.47 -0.1 51,092 + EX31_RS15170 0.47 +0.4 51,093 - EX31_RS15170 0.47 +1.2 51,093 - EX31_RS15170 0.47 +0.6 51,093 - EX31_RS15170 0.47 +0.4 51,093 - EX31_RS15170 0.47 -2.5 51,093 - EX31_RS15170 0.47 +0.5 51,093 - EX31_RS15170 0.47 -0.1 51,154 + EX31_RS15170 0.56 -0.1 51,154 + EX31_RS15170 0.56 -0.1 51,154 + EX31_RS15170 0.56 -0.5 51,155 - EX31_RS15170 0.56 -0.4 51,200 + EX31_RS15170 0.62 -0.2 51,200 + EX31_RS15170 0.62 -0.0 51,200 + EX31_RS15170 0.62 -0.1 51,201 - EX31_RS15170 0.62 +0.4 51,211 + EX31_RS15170 0.63 +0.4 51,211 + EX31_RS15170 0.63 -0.5 51,211 + EX31_RS15170 0.63 +0.4 51,211 + EX31_RS15170 0.63 +1.3 51,211 + EX31_RS15170 0.63 -0.1 51,211 + EX31_RS15170 0.63 -0.1 51,211 + EX31_RS15170 0.63 +2.4 51,211 + EX31_RS15170 0.63 -0.2 51,211 + EX31_RS15170 0.63 -0.1 51,212 - EX31_RS15170 0.64 -0.1 51,212 - EX31_RS15170 0.64 +0.7 51,248 + EX31_RS15170 0.69 -0.3 51,260 + EX31_RS15170 0.70 +1.0 51,260 + EX31_RS15170 0.70 -0.0 51,260 + EX31_RS15170 0.70 +1.4 51,260 + EX31_RS15170 0.70 -0.5 51,260 + EX31_RS15170 0.70 -0.3 51,261 - EX31_RS15170 0.70 -0.8 51,261 - EX31_RS15170 0.70 -0.5 51,261 - EX31_RS15170 0.70 -0.6 51,261 - EX31_RS15170 0.70 -0.4 51,261 - EX31_RS15170 0.70 +0.5 51,263 - EX31_RS15170 0.71 +0.1 51,263 - EX31_RS15170 0.71 -0.1 51,377 - EX31_RS15170 0.87 -0.0 51,431 - -1.5 51,468 + +0.6 51,468 + -0.6 51,468 + -0.3 51,468 + -0.6 51,468 + -0.1 51,468 + -0.4 51,468 + -0.3 51,468 + -0.6 51,468 + -0.3 51,468 + +0.1 51,469 - -0.3 51,469 - +1.2 51,469 - -0.6 51,469 - -0.9 51,469 - -0.4 51,469 - +1.8 51,469 - +0.7 51,469 - +0.6 51,469 - -0.3 51,469 - +0.9 51,469 - -0.3 51,471 - +0.3 51,481 + -0.3 51,495 + +0.5 51,568 + -0.2 51,569 - +0.7 51,569 - +1.6 51,732 - -0.1 51,736 + +0.0 51,736 + +0.1 51,736 + +0.3 51,736 + +2.0 51,736 + -0.4 51,736 + -0.8 51,737 - +0.1 51,737 - +0.1 51,737 - -0.1 51,737 - -1.6 51,737 - -0.6 51,739 + -0.2 51,739 + -0.9 51,740 - +0.3
Or see this region's nucleotide sequence