Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0300 and EX28DRAFT_0301 are separated by 13 nucleotides EX28DRAFT_0301 and EX28DRAFT_0302 are separated by 75 nucleotides EX28DRAFT_0302 and EX28DRAFT_0303 are separated by 140 nucleotides EX28DRAFT_0303 and EX28DRAFT_0304 are separated by 56 nucleotides
EX28DRAFT_0300: EX28DRAFT_0300 - TIGR01777 family protein, at 261,445 to 262,338
_0300
EX28DRAFT_0301: EX28DRAFT_0301 - FolB domain, at 262,352 to 262,720
_0301
EX28DRAFT_0302: EX28DRAFT_0302 - Glutathione S-transferase, at 262,796 to 263,425
_0302
EX28DRAFT_0303: EX28DRAFT_0303 - Glutathione S-transferase, at 263,566 to 264,210
_0303
EX28DRAFT_0304: EX28DRAFT_0304 - phosphoesterase, MJ0936 family, at 264,267 to 264,818
_0304
Position (kb)
262
263
264 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 262.088 kb on - strand, within EX28DRAFT_0300 at 262.147 kb on - strand, within EX28DRAFT_0300 at 262.339 kb on - strand at 262.339 kb on - strand at 262.353 kb on + strand at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.438 kb on - strand, within EX28DRAFT_0301 at 262.438 kb on - strand, within EX28DRAFT_0301 at 262.699 kb on + strand at 262.742 kb on + strand at 262.758 kb on + strand at 262.758 kb on + strand at 262.759 kb on - strand at 262.759 kb on - strand at 262.950 kb on + strand, within EX28DRAFT_0302 at 263.005 kb on - strand, within EX28DRAFT_0302 at 263.194 kb on - strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.328 kb on - strand, within EX28DRAFT_0302 at 263.328 kb on - strand, within EX28DRAFT_0302 at 263.329 kb on + strand, within EX28DRAFT_0302 at 263.329 kb on + strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.387 kb on - strand at 263.387 kb on - strand at 263.471 kb on - strand at 263.473 kb on - strand at 263.473 kb on - strand at 263.473 kb on - strand at 263.473 kb on - strand at 263.527 kb on + strand at 263.527 kb on + strand at 263.527 kb on + strand at 263.529 kb on + strand at 263.529 kb on + strand at 263.529 kb on + strand at 263.529 kb on + strand at 263.530 kb on - strand at 263.530 kb on - strand at 263.530 kb on - strand at 263.555 kb on - strand at 263.566 kb on - strand at 263.617 kb on + strand at 263.617 kb on + strand at 263.618 kb on - strand at 263.622 kb on + strand at 263.622 kb on + strand at 263.623 kb on - strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.631 kb on - strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.632 kb on + strand, within EX28DRAFT_0303 at 263.633 kb on - strand, within EX28DRAFT_0303 at 263.797 kb on + strand, within EX28DRAFT_0303 at 263.840 kb on - strand, within EX28DRAFT_0303 at 264.139 kb on + strand, within EX28DRAFT_0303 at 264.178 kb on + strand at 264.178 kb on + strand at 264.178 kb on + strand at 264.178 kb on + strand at 264.178 kb on + strand at 264.179 kb on - strand at 264.211 kb on + strand at 264.212 kb on - strand at 264.231 kb on - strand at 264.363 kb on - strand, within EX28DRAFT_0304
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 262,088 - EX28DRAFT_0300 0.72 -0.6 262,147 - EX28DRAFT_0300 0.79 +0.5 262,339 - -1.6 262,339 - -0.4 262,353 + +0.4 262,437 + EX28DRAFT_0301 0.23 +0.3 262,437 + EX28DRAFT_0301 0.23 +0.6 262,437 + EX28DRAFT_0301 0.23 -1.6 262,437 + EX28DRAFT_0301 0.23 +0.2 262,438 - EX28DRAFT_0301 0.23 -1.8 262,438 - EX28DRAFT_0301 0.23 -1.0 262,699 + -0.1 262,742 + +0.7 262,758 + +0.8 262,758 + -0.3 262,759 - -0.7 262,759 - -0.5 262,950 + EX28DRAFT_0302 0.24 +0.4 263,005 - EX28DRAFT_0302 0.33 -0.6 263,194 - EX28DRAFT_0302 0.63 -3.3 263,327 + EX28DRAFT_0302 0.84 -1.6 263,327 + EX28DRAFT_0302 0.84 +2.3 263,327 + EX28DRAFT_0302 0.84 -0.0 263,327 + EX28DRAFT_0302 0.84 +0.1 263,328 - EX28DRAFT_0302 0.84 -1.2 263,328 - EX28DRAFT_0302 0.84 +0.3 263,329 + EX28DRAFT_0302 0.85 -0.4 263,329 + EX28DRAFT_0302 0.85 -0.3 263,330 - EX28DRAFT_0302 0.85 -0.4 263,330 - EX28DRAFT_0302 0.85 +0.4 263,330 - EX28DRAFT_0302 0.85 +0.5 263,387 - -3.0 263,387 - -0.3 263,471 - -1.4 263,473 - +0.2 263,473 - -1.0 263,473 - -0.7 263,473 - +0.3 263,527 + +1.3 263,527 + -0.6 263,527 + -0.1 263,529 + +0.9 263,529 + +0.5 263,529 + +0.6 263,529 + -0.0 263,530 - +0.2 263,530 - +0.9 263,530 - +0.1 263,555 - -1.2 263,566 - -0.4 263,617 + +0.8 263,617 + +0.5 263,618 - +0.2 263,622 + +2.6 263,622 + +0.0 263,623 - +0.6 263,630 + +0.2 263,630 + -0.0 263,630 + -1.8 263,630 + +0.2 263,631 - EX28DRAFT_0303 0.10 -1.0 263,632 + EX28DRAFT_0303 0.10 +0.4 263,632 + EX28DRAFT_0303 0.10 -0.4 263,632 + EX28DRAFT_0303 0.10 -0.0 263,632 + EX28DRAFT_0303 0.10 +1.5 263,633 - EX28DRAFT_0303 0.10 +0.2 263,797 + EX28DRAFT_0303 0.36 +0.3 263,840 - EX28DRAFT_0303 0.42 +2.6 264,139 + EX28DRAFT_0303 0.89 +1.3 264,178 + -0.3 264,178 + +0.6 264,178 + +0.1 264,178 + +0.0 264,178 + -0.3 264,179 - +0.2 264,211 + -0.5 264,212 - +0.1 264,231 - -0.6 264,363 - EX28DRAFT_0304 0.17 -0.5
Or see this region's nucleotide sequence