Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18815 and EX31_RS18820 are separated by 23 nucleotides EX31_RS18820 and EX31_RS18825 are separated by 44 nucleotides EX31_RS18825 and EX31_RS18830 are separated by 236 nucleotides
EX31_RS18815: EX31_RS18815 - choline dehydrogenase, at 346,496 to 348,181
_RS18815
EX31_RS18820: EX31_RS18820 - betaine-aldehyde dehydrogenase, at 348,205 to 349,674
_RS18820
EX31_RS18825: EX31_RS18825 - transcriptional regulator BetI, at 349,719 to 350,318
_RS18825
EX31_RS18830: EX31_RS18830 - choline transporter, at 350,555 to 352,609
_RS18830
Position (kb) 348
349
350 Strain fitness (log2 ratio) -2
-1
0
1
2 at 347.257 kb on + strand, within EX31_RS18815 at 347.257 kb on + strand, within EX31_RS18815 at 347.258 kb on - strand, within EX31_RS18815 at 347.266 kb on - strand, within EX31_RS18815 at 347.266 kb on - strand, within EX31_RS18815 at 347.266 kb on - strand, within EX31_RS18815 at 347.266 kb on - strand, within EX31_RS18815 at 347.266 kb on - strand, within EX31_RS18815 at 347.266 kb on - strand, within EX31_RS18815 at 347.266 kb on - strand, within EX31_RS18815 at 347.454 kb on + strand, within EX31_RS18815 at 347.454 kb on + strand, within EX31_RS18815 at 347.455 kb on - strand, within EX31_RS18815 at 347.455 kb on - strand, within EX31_RS18815 at 347.455 kb on - strand, within EX31_RS18815 at 347.455 kb on - strand, within EX31_RS18815 at 347.455 kb on - strand, within EX31_RS18815 at 347.455 kb on - strand, within EX31_RS18815 at 347.473 kb on - strand, within EX31_RS18815 at 347.473 kb on - strand, within EX31_RS18815 at 347.674 kb on - strand, within EX31_RS18815 at 347.720 kb on - strand, within EX31_RS18815 at 347.720 kb on - strand, within EX31_RS18815 at 347.731 kb on + strand, within EX31_RS18815 at 347.811 kb on + strand, within EX31_RS18815 at 347.830 kb on - strand, within EX31_RS18815 at 347.830 kb on - strand, within EX31_RS18815 at 347.896 kb on - strand, within EX31_RS18815 at 347.896 kb on - strand, within EX31_RS18815 at 347.896 kb on - strand, within EX31_RS18815 at 347.968 kb on + strand, within EX31_RS18815 at 347.992 kb on - strand, within EX31_RS18815 at 347.992 kb on - strand, within EX31_RS18815 at 348.004 kb on - strand, within EX31_RS18815 at 348.067 kb on - strand at 348.135 kb on - strand at 348.135 kb on - strand at 348.135 kb on - strand at 348.135 kb on - strand at 348.168 kb on + strand at 348.168 kb on + strand at 348.169 kb on - strand at 348.169 kb on - strand at 348.175 kb on - strand at 348.212 kb on - strand at 348.260 kb on + strand at 348.260 kb on + strand at 348.261 kb on - strand at 348.261 kb on - strand at 348.261 kb on - strand at 348.441 kb on - strand, within EX31_RS18820 at 348.441 kb on - strand, within EX31_RS18820 at 348.485 kb on + strand, within EX31_RS18820 at 348.486 kb on - strand, within EX31_RS18820 at 348.486 kb on - strand, within EX31_RS18820 at 348.559 kb on + strand, within EX31_RS18820 at 348.559 kb on + strand, within EX31_RS18820 at 348.576 kb on - strand, within EX31_RS18820 at 348.576 kb on - strand, within EX31_RS18820 at 348.576 kb on - strand, within EX31_RS18820 at 348.576 kb on - strand, within EX31_RS18820 at 348.660 kb on - strand, within EX31_RS18820 at 348.660 kb on - strand, within EX31_RS18820 at 348.781 kb on - strand, within EX31_RS18820 at 348.781 kb on - strand, within EX31_RS18820 at 348.781 kb on - strand, within EX31_RS18820 at 348.781 kb on - strand, within EX31_RS18820 at 348.799 kb on - strand, within EX31_RS18820 at 348.799 kb on - strand, within EX31_RS18820 at 348.873 kb on + strand, within EX31_RS18820 at 349.100 kb on + strand, within EX31_RS18820 at 349.101 kb on - strand, within EX31_RS18820 at 349.266 kb on - strand, within EX31_RS18820 at 349.328 kb on + strand, within EX31_RS18820 at 349.329 kb on - strand, within EX31_RS18820 at 349.329 kb on - strand, within EX31_RS18820 at 349.331 kb on + strand, within EX31_RS18820 at 349.332 kb on - strand, within EX31_RS18820 at 349.332 kb on - strand, within EX31_RS18820 at 349.594 kb on - strand at 349.645 kb on - strand at 349.645 kb on - strand at 349.647 kb on - strand at 349.647 kb on - strand at 349.647 kb on - strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.758 kb on - strand at 349.909 kb on - strand, within EX31_RS18825 at 349.909 kb on - strand, within EX31_RS18825 at 349.909 kb on - strand, within EX31_RS18825 at 349.909 kb on - strand, within EX31_RS18825 at 349.919 kb on - strand, within EX31_RS18825 at 349.951 kb on + strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.278 kb on - strand at 350.278 kb on - strand at 350.278 kb on - strand at 350.278 kb on - strand at 350.278 kb on - strand at 350.308 kb on - strand at 350.308 kb on - strand at 350.308 kb on - strand at 350.308 kb on - strand at 350.371 kb on + strand at 350.371 kb on + strand at 350.372 kb on - strand at 350.393 kb on - strand at 350.393 kb on - strand at 350.393 kb on - strand at 350.413 kb on - strand at 350.455 kb on + strand at 350.455 kb on + strand at 350.456 kb on - strand at 350.456 kb on - strand at 350.470 kb on - strand at 350.470 kb on - strand at 350.470 kb on - strand at 350.478 kb on - strand at 350.532 kb on + strand at 350.532 kb on + strand at 350.541 kb on + strand at 350.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 347,257 + EX31_RS18815 0.45 -0.6 347,257 + EX31_RS18815 0.45 +0.8 347,258 - EX31_RS18815 0.45 +0.2 347,266 - EX31_RS18815 0.46 +0.1 347,266 - EX31_RS18815 0.46 +1.7 347,266 - EX31_RS18815 0.46 -0.0 347,266 - EX31_RS18815 0.46 -0.2 347,266 - EX31_RS18815 0.46 -0.3 347,266 - EX31_RS18815 0.46 +0.7 347,266 - EX31_RS18815 0.46 -0.6 347,454 + EX31_RS18815 0.57 +2.0 347,454 + EX31_RS18815 0.57 -0.3 347,455 - EX31_RS18815 0.57 -1.7 347,455 - EX31_RS18815 0.57 -0.3 347,455 - EX31_RS18815 0.57 +1.3 347,455 - EX31_RS18815 0.57 +0.8 347,455 - EX31_RS18815 0.57 -0.8 347,455 - EX31_RS18815 0.57 +0.3 347,473 - EX31_RS18815 0.58 -0.3 347,473 - EX31_RS18815 0.58 -0.0 347,674 - EX31_RS18815 0.70 -0.9 347,720 - EX31_RS18815 0.73 +0.0 347,720 - EX31_RS18815 0.73 -0.3 347,731 + EX31_RS18815 0.73 -0.0 347,811 + EX31_RS18815 0.78 +0.8 347,830 - EX31_RS18815 0.79 -0.8 347,830 - EX31_RS18815 0.79 -0.1 347,896 - EX31_RS18815 0.83 -0.2 347,896 - EX31_RS18815 0.83 +0.7 347,896 - EX31_RS18815 0.83 -0.3 347,968 + EX31_RS18815 0.87 +2.0 347,992 - EX31_RS18815 0.89 +0.3 347,992 - EX31_RS18815 0.89 +0.5 348,004 - EX31_RS18815 0.89 +0.8 348,067 - +0.1 348,135 - +0.4 348,135 - +0.2 348,135 - -0.3 348,135 - -0.0 348,168 + -2.4 348,168 + -0.5 348,169 - -0.3 348,169 - +0.4 348,175 - +0.8 348,212 - -0.0 348,260 + +0.7 348,260 + +1.6 348,261 - -1.3 348,261 - -0.6 348,261 - +0.2 348,441 - EX31_RS18820 0.16 -0.3 348,441 - EX31_RS18820 0.16 -0.5 348,485 + EX31_RS18820 0.19 -0.3 348,486 - EX31_RS18820 0.19 -0.1 348,486 - EX31_RS18820 0.19 -0.3 348,559 + EX31_RS18820 0.24 +1.6 348,559 + EX31_RS18820 0.24 -0.0 348,576 - EX31_RS18820 0.25 +0.1 348,576 - EX31_RS18820 0.25 -0.3 348,576 - EX31_RS18820 0.25 -0.7 348,576 - EX31_RS18820 0.25 -0.5 348,660 - EX31_RS18820 0.31 -0.2 348,660 - EX31_RS18820 0.31 +1.0 348,781 - EX31_RS18820 0.39 +0.6 348,781 - EX31_RS18820 0.39 -0.4 348,781 - EX31_RS18820 0.39 -1.4 348,781 - EX31_RS18820 0.39 +0.3 348,799 - EX31_RS18820 0.40 -2.2 348,799 - EX31_RS18820 0.40 -1.6 348,873 + EX31_RS18820 0.45 -0.5 349,100 + EX31_RS18820 0.61 -2.5 349,101 - EX31_RS18820 0.61 +0.4 349,266 - EX31_RS18820 0.72 +0.3 349,328 + EX31_RS18820 0.76 -0.1 349,329 - EX31_RS18820 0.76 +0.3 349,329 - EX31_RS18820 0.76 +0.4 349,331 + EX31_RS18820 0.77 -1.3 349,332 - EX31_RS18820 0.77 -1.0 349,332 - EX31_RS18820 0.77 +0.4 349,594 - +2.0 349,645 - +1.5 349,645 - +2.4 349,647 - +1.4 349,647 - -0.2 349,647 - +0.9 349,674 + -0.3 349,674 + -1.5 349,674 + -0.2 349,674 + +0.5 349,674 + -1.3 349,674 + +0.4 349,674 + -0.2 349,674 + -1.3 349,674 + +0.5 349,674 + -0.8 349,674 + +1.0 349,674 + +1.2 349,674 + -0.2 349,674 + +0.2 349,674 + +0.6 349,674 + -0.4 349,675 - -0.2 349,675 - -1.7 349,675 - +0.1 349,675 - -0.1 349,675 - -0.1 349,675 - -0.3 349,675 - -0.3 349,675 - -0.6 349,675 - +0.2 349,675 - -0.0 349,675 - +1.1 349,675 - -0.8 349,675 - -0.3 349,675 - -0.1 349,675 - +0.1 349,675 - -1.0 349,675 - +0.7 349,675 - -0.2 349,675 - -0.2 349,675 - -0.3 349,758 - -0.2 349,909 - EX31_RS18825 0.32 -0.6 349,909 - EX31_RS18825 0.32 -0.1 349,909 - EX31_RS18825 0.32 -1.2 349,909 - EX31_RS18825 0.32 +0.3 349,919 - EX31_RS18825 0.33 -1.6 349,951 + EX31_RS18825 0.39 +2.0 350,139 - EX31_RS18825 0.70 -0.5 350,139 - EX31_RS18825 0.70 +0.6 350,139 - EX31_RS18825 0.70 -0.8 350,139 - EX31_RS18825 0.70 -0.7 350,278 - -0.5 350,278 - -0.4 350,278 - -2.4 350,278 - -0.1 350,278 - +0.4 350,308 - -0.7 350,308 - +0.4 350,308 - -0.4 350,308 - -0.2 350,371 + -0.0 350,371 + +0.7 350,372 - -0.5 350,393 - -0.8 350,393 - +1.1 350,393 - -0.6 350,413 - -0.5 350,455 + +0.1 350,455 + +0.7 350,456 - +0.1 350,456 - +1.0 350,470 - +0.2 350,470 - -0.5 350,470 - +0.3 350,478 - -1.0 350,532 + +1.3 350,532 + -0.2 350,541 + -0.0 350,542 - -0.2
Or see this region's nucleotide sequence