Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_2020

Experiment: M9 with sucrose; no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_2018 and EX28DRAFT_2019 overlap by 4 nucleotidesEX28DRAFT_2019 and EX28DRAFT_2020 are separated by 45 nucleotidesEX28DRAFT_2020 and EX28DRAFT_2021 are separated by 44 nucleotides EX28DRAFT_2018: EX28DRAFT_2018 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 2,028,512 to 2,029,258 _2018 EX28DRAFT_2019: EX28DRAFT_2019 - Uncharacterized conserved protein, at 2,029,255 to 2,029,437 _2019 EX28DRAFT_2020: EX28DRAFT_2020 - Uncharacterized protein conserved in bacteria, at 2,029,483 to 2,030,712 _2020 EX28DRAFT_2021: EX28DRAFT_2021 - lipid-A-disaccharide kinase (EC 2.7.1.130), at 2,030,757 to 2,031,734 _2021 Position (kb) 2029 2030 2031Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2028.514 kb on - strandat 2029.469 kb on - strandat 2029.646 kb on + strand, within EX28DRAFT_2020at 2029.772 kb on + strand, within EX28DRAFT_2020at 2029.772 kb on + strand, within EX28DRAFT_2020at 2029.773 kb on - strand, within EX28DRAFT_2020at 2029.861 kb on - strand, within EX28DRAFT_2020at 2030.316 kb on + strand, within EX28DRAFT_2020at 2030.403 kb on + strand, within EX28DRAFT_2020at 2030.470 kb on + strand, within EX28DRAFT_2020at 2030.529 kb on + strand, within EX28DRAFT_2020at 2030.543 kb on + strand, within EX28DRAFT_2020at 2030.565 kb on + strand, within EX28DRAFT_2020at 2030.671 kb on + strandat 2030.671 kb on + strandat 2030.672 kb on - strandat 2030.672 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control
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2,028,514 - -0.5
2,029,469 - +0.8
2,029,646 + EX28DRAFT_2020 0.13 +0.3
2,029,772 + EX28DRAFT_2020 0.23 +0.1
2,029,772 + EX28DRAFT_2020 0.23 +0.2
2,029,773 - EX28DRAFT_2020 0.24 +0.5
2,029,861 - EX28DRAFT_2020 0.31 +0.7
2,030,316 + EX28DRAFT_2020 0.68 +1.0
2,030,403 + EX28DRAFT_2020 0.75 +2.1
2,030,470 + EX28DRAFT_2020 0.80 -2.9
2,030,529 + EX28DRAFT_2020 0.85 +0.0
2,030,543 + EX28DRAFT_2020 0.86 +1.1
2,030,565 + EX28DRAFT_2020 0.88 -0.2
2,030,671 + +1.1
2,030,671 + +0.2
2,030,672 - -0.2
2,030,672 - -0.7

Or see this region's nucleotide sequence