Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1503 and EX28DRAFT_1504 are separated by 150 nucleotides EX28DRAFT_1504 and EX28DRAFT_1505 are separated by 159 nucleotides EX28DRAFT_1505 and EX28DRAFT_1506 are separated by 174 nucleotides
EX28DRAFT_1503: EX28DRAFT_1503 - 4-aminobutyrate aminotransferase, prokaryotic type, at 1,518,492 to 1,519,763
_1503
EX28DRAFT_1504: EX28DRAFT_1504 - Zn-dependent carboxypeptidase, at 1,519,914 to 1,521,401
_1504
EX28DRAFT_1505: EX28DRAFT_1505 - Thioredoxin-like domain, at 1,521,561 to 1,522,097
_1505
EX28DRAFT_1506: EX28DRAFT_1506 - Acid shock protein repeat, at 1,522,272 to 1,522,736
_1506
Position (kb)
1519
1520
1521
1522 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.915 kb on + strand, within EX28DRAFT_1503 at 1518.916 kb on - strand, within EX28DRAFT_1503 at 1518.916 kb on - strand, within EX28DRAFT_1503 at 1518.917 kb on + strand, within EX28DRAFT_1503 at 1518.918 kb on - strand, within EX28DRAFT_1503 at 1518.954 kb on - strand, within EX28DRAFT_1503 at 1518.988 kb on - strand, within EX28DRAFT_1503 at 1519.381 kb on - strand, within EX28DRAFT_1503 at 1519.427 kb on + strand, within EX28DRAFT_1503 at 1519.427 kb on + strand, within EX28DRAFT_1503 at 1519.428 kb on - strand, within EX28DRAFT_1503 at 1519.428 kb on - strand, within EX28DRAFT_1503 at 1519.745 kb on + strand at 1519.762 kb on - strand at 1519.762 kb on - strand at 1519.762 kb on - strand at 1519.762 kb on - strand at 1519.824 kb on + strand at 1519.825 kb on - strand at 1519.873 kb on + strand at 1519.874 kb on - strand at 1520.662 kb on - strand, within EX28DRAFT_1504 at 1520.846 kb on + strand, within EX28DRAFT_1504 at 1520.943 kb on + strand, within EX28DRAFT_1504 at 1520.944 kb on - strand, within EX28DRAFT_1504 at 1520.944 kb on - strand, within EX28DRAFT_1504 at 1520.944 kb on - strand, within EX28DRAFT_1504 at 1520.944 kb on - strand, within EX28DRAFT_1504 at 1520.997 kb on + strand, within EX28DRAFT_1504 at 1521.119 kb on + strand, within EX28DRAFT_1504 at 1521.119 kb on + strand, within EX28DRAFT_1504 at 1521.169 kb on - strand, within EX28DRAFT_1504 at 1521.169 kb on - strand, within EX28DRAFT_1504 at 1521.186 kb on + strand, within EX28DRAFT_1504 at 1521.187 kb on - strand, within EX28DRAFT_1504 at 1521.328 kb on + strand at 1521.359 kb on + strand at 1521.360 kb on - strand at 1521.490 kb on + strand at 1521.490 kb on + strand at 1521.517 kb on + strand at 1521.518 kb on - strand at 1521.720 kb on - strand, within EX28DRAFT_1505 at 1521.753 kb on + strand, within EX28DRAFT_1505 at 1521.767 kb on - strand, within EX28DRAFT_1505 at 1521.786 kb on + strand, within EX28DRAFT_1505 at 1521.788 kb on + strand, within EX28DRAFT_1505 at 1521.788 kb on + strand, within EX28DRAFT_1505 at 1521.789 kb on - strand, within EX28DRAFT_1505 at 1521.860 kb on + strand, within EX28DRAFT_1505 at 1521.860 kb on + strand, within EX28DRAFT_1505 at 1522.052 kb on - strand at 1522.052 kb on - strand at 1522.095 kb on + strand at 1522.135 kb on + strand at 1522.136 kb on - strand at 1522.154 kb on + strand at 1522.212 kb on + strand at 1522.212 kb on + strand at 1522.213 kb on - strand at 1522.213 kb on - strand at 1522.213 kb on - strand at 1522.213 kb on - strand at 1522.213 kb on - strand at 1522.217 kb on - strand at 1522.277 kb on + strand at 1522.278 kb on - strand at 1522.282 kb on + strand at 1522.283 kb on - strand at 1522.285 kb on + strand at 1522.285 kb on + strand at 1522.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,518,915 + EX28DRAFT_1503 0.33 -1.7 1,518,915 + EX28DRAFT_1503 0.33 -0.4 1,518,915 + EX28DRAFT_1503 0.33 -0.8 1,518,915 + EX28DRAFT_1503 0.33 -0.9 1,518,916 - EX28DRAFT_1503 0.33 +0.4 1,518,916 - EX28DRAFT_1503 0.33 -0.3 1,518,917 + EX28DRAFT_1503 0.33 +1.3 1,518,918 - EX28DRAFT_1503 0.33 +0.7 1,518,954 - EX28DRAFT_1503 0.36 +0.1 1,518,988 - EX28DRAFT_1503 0.39 +0.0 1,519,381 - EX28DRAFT_1503 0.70 -1.1 1,519,427 + EX28DRAFT_1503 0.74 +0.1 1,519,427 + EX28DRAFT_1503 0.74 -1.6 1,519,428 - EX28DRAFT_1503 0.74 -0.1 1,519,428 - EX28DRAFT_1503 0.74 +1.2 1,519,745 + +0.0 1,519,762 - +0.7 1,519,762 - -0.9 1,519,762 - +3.8 1,519,762 - +0.8 1,519,824 + -1.6 1,519,825 - +0.8 1,519,873 + -0.4 1,519,874 - +0.2 1,520,662 - EX28DRAFT_1504 0.50 +0.0 1,520,846 + EX28DRAFT_1504 0.63 -0.3 1,520,943 + EX28DRAFT_1504 0.69 -0.4 1,520,944 - EX28DRAFT_1504 0.69 +1.1 1,520,944 - EX28DRAFT_1504 0.69 -0.8 1,520,944 - EX28DRAFT_1504 0.69 +1.3 1,520,944 - EX28DRAFT_1504 0.69 -0.7 1,520,997 + EX28DRAFT_1504 0.73 +0.9 1,521,119 + EX28DRAFT_1504 0.81 +0.7 1,521,119 + EX28DRAFT_1504 0.81 +0.5 1,521,169 - EX28DRAFT_1504 0.84 +0.8 1,521,169 - EX28DRAFT_1504 0.84 -0.4 1,521,186 + EX28DRAFT_1504 0.85 +0.0 1,521,187 - EX28DRAFT_1504 0.86 -0.2 1,521,328 + -1.1 1,521,359 + +0.2 1,521,360 - +1.2 1,521,490 + -0.4 1,521,490 + +0.1 1,521,517 + -0.1 1,521,518 - -0.4 1,521,720 - EX28DRAFT_1505 0.30 +1.0 1,521,753 + EX28DRAFT_1505 0.36 -0.5 1,521,767 - EX28DRAFT_1505 0.38 +0.3 1,521,786 + EX28DRAFT_1505 0.42 -0.2 1,521,788 + EX28DRAFT_1505 0.42 +0.1 1,521,788 + EX28DRAFT_1505 0.42 -2.1 1,521,789 - EX28DRAFT_1505 0.42 -0.1 1,521,860 + EX28DRAFT_1505 0.56 -0.2 1,521,860 + EX28DRAFT_1505 0.56 -1.5 1,522,052 - -0.6 1,522,052 - +0.1 1,522,095 + +0.5 1,522,135 + -0.6 1,522,136 - -3.0 1,522,154 + -2.1 1,522,212 + -0.0 1,522,212 + -0.4 1,522,213 - +1.2 1,522,213 - +0.1 1,522,213 - +1.8 1,522,213 - -0.7 1,522,213 - +0.2 1,522,217 - -0.3 1,522,277 + +0.1 1,522,278 - -1.7 1,522,282 + +0.2 1,522,283 - -0.3 1,522,285 + +0.0 1,522,285 + +0.6 1,522,286 - -0.4
Or see this region's nucleotide sequence