Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09995 and CA265_RS10000 are separated by 37 nucleotides CA265_RS10000 and CA265_RS10005 are separated by 106 nucleotides CA265_RS10005 and CA265_RS10010 are separated by 32 nucleotides
CA265_RS09995: CA265_RS09995 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 2,456,661 to 2,459,174
_RS09995
CA265_RS10000: CA265_RS10000 - nitrite reductase small subunit, at 2,459,212 to 2,459,559
_RS10000
CA265_RS10005: CA265_RS10005 - hypothetical protein, at 2,459,666 to 2,460,013
_RS10005
CA265_RS10010: CA265_RS10010 - MFS transporter, at 2,460,046 to 2,461,410
_RS10010
Position (kb)
2459
2460 Strain fitness (log2 ratio)
-1
0
1 at 2458.350 kb on + strand, within CA265_RS09995 at 2458.400 kb on - strand, within CA265_RS09995 at 2458.400 kb on - strand, within CA265_RS09995 at 2458.406 kb on - strand, within CA265_RS09995 at 2458.418 kb on - strand, within CA265_RS09995 at 2458.428 kb on + strand, within CA265_RS09995 at 2458.438 kb on - strand, within CA265_RS09995 at 2458.517 kb on - strand, within CA265_RS09995 at 2458.589 kb on + strand, within CA265_RS09995 at 2458.616 kb on + strand, within CA265_RS09995 at 2458.616 kb on + strand, within CA265_RS09995 at 2458.616 kb on + strand, within CA265_RS09995 at 2458.616 kb on + strand, within CA265_RS09995 at 2458.617 kb on - strand, within CA265_RS09995 at 2458.651 kb on - strand, within CA265_RS09995 at 2458.770 kb on + strand, within CA265_RS09995 at 2458.770 kb on + strand, within CA265_RS09995 at 2458.770 kb on + strand, within CA265_RS09995 at 2458.770 kb on + strand, within CA265_RS09995 at 2458.771 kb on - strand, within CA265_RS09995 at 2458.771 kb on - strand, within CA265_RS09995 at 2458.771 kb on - strand, within CA265_RS09995 at 2458.775 kb on - strand, within CA265_RS09995 at 2458.775 kb on - strand, within CA265_RS09995 at 2458.958 kb on - strand at 2458.958 kb on - strand at 2458.961 kb on + strand at 2458.962 kb on - strand at 2458.971 kb on - strand at 2459.089 kb on + strand at 2459.089 kb on + strand at 2459.198 kb on + strand at 2459.381 kb on + strand, within CA265_RS10000 at 2459.511 kb on - strand, within CA265_RS10000 at 2459.629 kb on + strand at 2459.633 kb on + strand at 2459.637 kb on + strand at 2459.638 kb on - strand at 2459.662 kb on + strand at 2459.684 kb on - strand at 2459.717 kb on - strand, within CA265_RS10005 at 2459.719 kb on + strand, within CA265_RS10005 at 2459.752 kb on + strand, within CA265_RS10005 at 2459.795 kb on + strand, within CA265_RS10005 at 2459.815 kb on - strand, within CA265_RS10005 at 2459.929 kb on - strand, within CA265_RS10005 at 2459.945 kb on + strand, within CA265_RS10005 at 2459.945 kb on + strand, within CA265_RS10005 at 2460.034 kb on - strand at 2460.045 kb on + strand at 2460.046 kb on - strand at 2460.057 kb on + strand at 2460.059 kb on - strand at 2460.076 kb on - strand at 2460.076 kb on - strand at 2460.081 kb on + strand at 2460.086 kb on + strand at 2460.229 kb on - strand, within CA265_RS10010 at 2460.261 kb on + strand, within CA265_RS10010 at 2460.333 kb on - strand, within CA265_RS10010 at 2460.377 kb on - strand, within CA265_RS10010 at 2460.414 kb on - strand, within CA265_RS10010 at 2460.414 kb on - strand, within CA265_RS10010 at 2460.456 kb on + strand, within CA265_RS10010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 2,458,350 + CA265_RS09995 0.67 -0.8 2,458,400 - CA265_RS09995 0.69 +0.7 2,458,400 - CA265_RS09995 0.69 +0.3 2,458,406 - CA265_RS09995 0.69 +0.0 2,458,418 - CA265_RS09995 0.70 -0.3 2,458,428 + CA265_RS09995 0.70 -0.2 2,458,438 - CA265_RS09995 0.71 +0.2 2,458,517 - CA265_RS09995 0.74 -0.7 2,458,589 + CA265_RS09995 0.77 +0.8 2,458,616 + CA265_RS09995 0.78 +0.1 2,458,616 + CA265_RS09995 0.78 -0.5 2,458,616 + CA265_RS09995 0.78 +0.4 2,458,616 + CA265_RS09995 0.78 -0.9 2,458,617 - CA265_RS09995 0.78 -0.3 2,458,651 - CA265_RS09995 0.79 +0.1 2,458,770 + CA265_RS09995 0.84 -0.4 2,458,770 + CA265_RS09995 0.84 +0.5 2,458,770 + CA265_RS09995 0.84 -0.1 2,458,770 + CA265_RS09995 0.84 -0.9 2,458,771 - CA265_RS09995 0.84 +0.5 2,458,771 - CA265_RS09995 0.84 +0.2 2,458,771 - CA265_RS09995 0.84 +0.1 2,458,775 - CA265_RS09995 0.84 +0.1 2,458,775 - CA265_RS09995 0.84 -1.0 2,458,958 - -0.5 2,458,958 - -0.8 2,458,961 + +0.1 2,458,962 - -0.3 2,458,971 - -1.2 2,459,089 + -0.6 2,459,089 + -0.1 2,459,198 + +1.0 2,459,381 + CA265_RS10000 0.49 -0.4 2,459,511 - CA265_RS10000 0.86 -1.0 2,459,629 + -0.6 2,459,633 + -1.2 2,459,637 + +1.4 2,459,638 - -0.5 2,459,662 + +0.4 2,459,684 - -0.8 2,459,717 - CA265_RS10005 0.15 +0.2 2,459,719 + CA265_RS10005 0.15 -0.9 2,459,752 + CA265_RS10005 0.25 -0.8 2,459,795 + CA265_RS10005 0.37 -0.5 2,459,815 - CA265_RS10005 0.43 +0.0 2,459,929 - CA265_RS10005 0.76 -0.1 2,459,945 + CA265_RS10005 0.80 -0.1 2,459,945 + CA265_RS10005 0.80 +1.4 2,460,034 - +0.4 2,460,045 + -0.2 2,460,046 - +1.3 2,460,057 + +0.1 2,460,059 - -0.7 2,460,076 - +0.2 2,460,076 - -0.6 2,460,081 + -0.1 2,460,086 + +0.3 2,460,229 - CA265_RS10010 0.13 -0.7 2,460,261 + CA265_RS10010 0.16 +0.8 2,460,333 - CA265_RS10010 0.21 -0.0 2,460,377 - CA265_RS10010 0.24 +0.7 2,460,414 - CA265_RS10010 0.27 -0.4 2,460,414 - CA265_RS10010 0.27 +0.2 2,460,456 + CA265_RS10010 0.30 -0.2
Or see this region's nucleotide sequence