Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09565 and CA265_RS09570 are separated by 62 nucleotides CA265_RS09570 and CA265_RS09575 are separated by 15 nucleotides CA265_RS09575 and CA265_RS09580 are separated by 237 nucleotides
CA265_RS09565: CA265_RS09565 - galactokinase, at 2,366,803 to 2,367,969
_RS09565
CA265_RS09570: CA265_RS09570 - RNA methyltransferase, at 2,368,032 to 2,368,562
_RS09570
CA265_RS09575: CA265_RS09575 - hypothetical protein, at 2,368,578 to 2,369,243
_RS09575
CA265_RS09580: CA265_RS09580 - flagellar motor protein MotB, at 2,369,481 to 2,370,806
_RS09580
Position (kb)
2368
2369 Strain fitness (log2 ratio)
-2
-1
0
1 at 2367.057 kb on + strand, within CA265_RS09565 at 2367.063 kb on - strand, within CA265_RS09565 at 2367.063 kb on - strand, within CA265_RS09565 at 2367.195 kb on - strand, within CA265_RS09565 at 2367.216 kb on + strand, within CA265_RS09565 at 2367.217 kb on - strand, within CA265_RS09565 at 2367.220 kb on + strand, within CA265_RS09565 at 2367.221 kb on - strand, within CA265_RS09565 at 2367.225 kb on - strand, within CA265_RS09565 at 2367.225 kb on - strand, within CA265_RS09565 at 2367.230 kb on + strand, within CA265_RS09565 at 2367.356 kb on + strand, within CA265_RS09565 at 2367.372 kb on - strand, within CA265_RS09565 at 2367.526 kb on + strand, within CA265_RS09565 at 2367.593 kb on + strand, within CA265_RS09565 at 2367.615 kb on + strand, within CA265_RS09565 at 2367.616 kb on - strand, within CA265_RS09565 at 2367.645 kb on + strand, within CA265_RS09565 at 2367.646 kb on - strand, within CA265_RS09565 at 2367.691 kb on + strand, within CA265_RS09565 at 2367.692 kb on - strand, within CA265_RS09565 at 2367.710 kb on + strand, within CA265_RS09565 at 2367.719 kb on + strand, within CA265_RS09565 at 2367.719 kb on + strand, within CA265_RS09565 at 2367.719 kb on + strand, within CA265_RS09565 at 2367.720 kb on - strand, within CA265_RS09565 at 2367.721 kb on + strand, within CA265_RS09565 at 2367.782 kb on + strand, within CA265_RS09565 at 2367.782 kb on + strand, within CA265_RS09565 at 2367.881 kb on - strand at 2367.917 kb on - strand at 2367.971 kb on + strand at 2368.109 kb on - strand, within CA265_RS09570 at 2368.193 kb on - strand, within CA265_RS09570 at 2368.205 kb on + strand, within CA265_RS09570 at 2368.206 kb on - strand, within CA265_RS09570 at 2368.246 kb on + strand, within CA265_RS09570 at 2368.274 kb on - strand, within CA265_RS09570 at 2368.318 kb on + strand, within CA265_RS09570 at 2368.319 kb on - strand, within CA265_RS09570 at 2368.367 kb on - strand, within CA265_RS09570 at 2368.399 kb on - strand, within CA265_RS09570 at 2368.451 kb on + strand, within CA265_RS09570 at 2368.547 kb on + strand at 2368.560 kb on + strand at 2368.629 kb on - strand at 2368.638 kb on - strand at 2368.638 kb on - strand at 2368.685 kb on + strand, within CA265_RS09575 at 2368.698 kb on + strand, within CA265_RS09575 at 2368.772 kb on + strand, within CA265_RS09575 at 2368.790 kb on + strand, within CA265_RS09575 at 2368.790 kb on + strand, within CA265_RS09575 at 2368.791 kb on - strand, within CA265_RS09575 at 2368.791 kb on - strand, within CA265_RS09575 at 2368.791 kb on - strand, within CA265_RS09575 at 2368.822 kb on - strand at 2368.949 kb on - strand, within CA265_RS09575 at 2368.998 kb on - strand, within CA265_RS09575 at 2369.022 kb on - strand, within CA265_RS09575 at 2369.041 kb on + strand, within CA265_RS09575 at 2369.042 kb on - strand, within CA265_RS09575 at 2369.120 kb on + strand, within CA265_RS09575 at 2369.257 kb on - strand at 2369.291 kb on + strand at 2369.291 kb on + strand at 2369.322 kb on + strand at 2369.323 kb on - strand at 2369.358 kb on - strand at 2369.368 kb on + strand at 2369.369 kb on - strand at 2369.376 kb on + strand at 2369.377 kb on - strand at 2369.434 kb on - strand at 2369.441 kb on - strand at 2369.458 kb on + strand at 2369.524 kb on + strand at 2369.525 kb on - strand at 2369.530 kb on + strand at 2369.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 2,367,057 + CA265_RS09565 0.22 +0.6 2,367,063 - CA265_RS09565 0.22 -0.2 2,367,063 - CA265_RS09565 0.22 -1.5 2,367,195 - CA265_RS09565 0.34 +0.3 2,367,216 + CA265_RS09565 0.35 +0.6 2,367,217 - CA265_RS09565 0.35 +0.1 2,367,220 + CA265_RS09565 0.36 -1.2 2,367,221 - CA265_RS09565 0.36 -0.9 2,367,225 - CA265_RS09565 0.36 -0.3 2,367,225 - CA265_RS09565 0.36 -0.2 2,367,230 + CA265_RS09565 0.37 -0.6 2,367,356 + CA265_RS09565 0.47 +0.4 2,367,372 - CA265_RS09565 0.49 +1.1 2,367,526 + CA265_RS09565 0.62 +0.5 2,367,593 + CA265_RS09565 0.68 -0.6 2,367,615 + CA265_RS09565 0.70 +0.4 2,367,616 - CA265_RS09565 0.70 -0.5 2,367,645 + CA265_RS09565 0.72 -1.3 2,367,646 - CA265_RS09565 0.72 -0.8 2,367,691 + CA265_RS09565 0.76 -0.7 2,367,692 - CA265_RS09565 0.76 -0.2 2,367,710 + CA265_RS09565 0.78 -0.1 2,367,719 + CA265_RS09565 0.78 +0.5 2,367,719 + CA265_RS09565 0.78 -0.2 2,367,719 + CA265_RS09565 0.78 -1.4 2,367,720 - CA265_RS09565 0.79 +0.0 2,367,721 + CA265_RS09565 0.79 -0.5 2,367,782 + CA265_RS09565 0.84 -1.1 2,367,782 + CA265_RS09565 0.84 -0.6 2,367,881 - +0.1 2,367,917 - +0.3 2,367,971 + -0.3 2,368,109 - CA265_RS09570 0.15 -1.3 2,368,193 - CA265_RS09570 0.30 -0.7 2,368,205 + CA265_RS09570 0.33 -0.6 2,368,206 - CA265_RS09570 0.33 -1.0 2,368,246 + CA265_RS09570 0.40 -1.4 2,368,274 - CA265_RS09570 0.46 +0.0 2,368,318 + CA265_RS09570 0.54 -2.7 2,368,319 - CA265_RS09570 0.54 -1.5 2,368,367 - CA265_RS09570 0.63 -0.7 2,368,399 - CA265_RS09570 0.69 +0.2 2,368,451 + CA265_RS09570 0.79 -0.1 2,368,547 + -0.5 2,368,560 + -0.2 2,368,629 - -0.6 2,368,638 - +0.5 2,368,638 - +0.9 2,368,685 + CA265_RS09575 0.16 -0.8 2,368,698 + CA265_RS09575 0.18 -0.4 2,368,772 + CA265_RS09575 0.29 +0.5 2,368,790 + CA265_RS09575 0.32 -0.4 2,368,790 + CA265_RS09575 0.32 +0.3 2,368,791 - CA265_RS09575 0.32 +0.2 2,368,791 - CA265_RS09575 0.32 -0.3 2,368,791 - CA265_RS09575 0.32 -0.3 2,368,822 - +1.3 2,368,949 - CA265_RS09575 0.56 +0.4 2,368,998 - CA265_RS09575 0.63 +0.2 2,369,022 - CA265_RS09575 0.67 +0.1 2,369,041 + CA265_RS09575 0.70 +0.6 2,369,042 - CA265_RS09575 0.70 +0.3 2,369,120 + CA265_RS09575 0.81 -0.2 2,369,257 - +1.3 2,369,291 + +0.0 2,369,291 + -0.0 2,369,322 + -1.0 2,369,323 - +0.4 2,369,358 - +0.3 2,369,368 + -0.0 2,369,369 - -0.9 2,369,376 + +0.9 2,369,377 - +0.0 2,369,434 - +0.1 2,369,441 - -0.5 2,369,458 + +0.3 2,369,524 + -0.1 2,369,525 - -0.0 2,369,530 + +0.1 2,369,543 - -0.3
Or see this region's nucleotide sequence