Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03610 and CA265_RS03615 are separated by 101 nucleotides CA265_RS03615 and CA265_RS03620 are separated by 1 nucleotides CA265_RS03620 and CA265_RS03625 are separated by 66 nucleotides
CA265_RS03610: CA265_RS03610 - imidazole glycerol phosphate synthase subunit HisF, at 863,308 to 864,447
_RS03610
CA265_RS03615: CA265_RS03615 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 864,549 to 865,298
_RS03615
CA265_RS03620: CA265_RS03620 - imidazole glycerol phosphate synthase subunit HisH, at 865,300 to 865,932
_RS03620
CA265_RS03625: CA265_RS03625 - bifunctional imidazole glycerol-phosphate dehydratase/histidinol phosphatase, at 865,999 to 867,132
_RS03625
Position (kb) 864
865
866 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 863.575 kb on + strand, within CA265_RS03610 at 863.684 kb on - strand, within CA265_RS03610 at 863.786 kb on - strand, within CA265_RS03610 at 863.788 kb on + strand, within CA265_RS03610 at 863.865 kb on + strand, within CA265_RS03610 at 863.866 kb on - strand at 863.907 kb on + strand, within CA265_RS03610 at 863.987 kb on - strand, within CA265_RS03610 at 864.055 kb on - strand, within CA265_RS03610 at 864.057 kb on + strand, within CA265_RS03610 at 864.168 kb on + strand, within CA265_RS03610 at 864.214 kb on - strand, within CA265_RS03610 at 864.300 kb on + strand, within CA265_RS03610 at 864.313 kb on - strand, within CA265_RS03610 at 864.352 kb on - strand at 864.490 kb on + strand at 864.584 kb on + strand at 864.631 kb on - strand, within CA265_RS03615 at 864.633 kb on + strand, within CA265_RS03615 at 864.633 kb on + strand, within CA265_RS03615 at 864.708 kb on - strand, within CA265_RS03615 at 864.782 kb on + strand, within CA265_RS03615 at 864.788 kb on + strand, within CA265_RS03615 at 864.789 kb on - strand, within CA265_RS03615 at 864.792 kb on + strand, within CA265_RS03615 at 864.879 kb on + strand, within CA265_RS03615 at 864.915 kb on - strand, within CA265_RS03615 at 864.992 kb on + strand, within CA265_RS03615 at 865.044 kb on + strand, within CA265_RS03615 at 865.121 kb on + strand, within CA265_RS03615 at 865.143 kb on + strand, within CA265_RS03615 at 865.154 kb on + strand, within CA265_RS03615 at 865.154 kb on + strand, within CA265_RS03615 at 865.154 kb on + strand, within CA265_RS03615 at 865.154 kb on + strand, within CA265_RS03615 at 865.184 kb on - strand, within CA265_RS03615 at 865.216 kb on + strand, within CA265_RS03615 at 865.281 kb on - strand at 865.290 kb on + strand at 865.291 kb on - strand at 865.291 kb on - strand at 865.291 kb on - strand at 865.295 kb on - strand at 865.302 kb on - strand at 865.302 kb on - strand at 865.454 kb on + strand, within CA265_RS03620 at 865.461 kb on + strand, within CA265_RS03620 at 865.481 kb on + strand, within CA265_RS03620 at 865.482 kb on - strand, within CA265_RS03620 at 865.491 kb on + strand, within CA265_RS03620 at 865.494 kb on + strand, within CA265_RS03620 at 865.494 kb on + strand, within CA265_RS03620 at 865.513 kb on + strand, within CA265_RS03620 at 865.612 kb on + strand, within CA265_RS03620 at 865.807 kb on + strand, within CA265_RS03620 at 865.817 kb on + strand, within CA265_RS03620 at 865.837 kb on + strand, within CA265_RS03620 at 865.838 kb on - strand, within CA265_RS03620 at 865.942 kb on + strand at 865.944 kb on + strand at 865.954 kb on - strand at 866.001 kb on - strand at 866.134 kb on + strand, within CA265_RS03625 at 866.157 kb on + strand, within CA265_RS03625 at 866.178 kb on - strand, within CA265_RS03625
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 863,575 + CA265_RS03610 0.23 -0.2 863,684 - CA265_RS03610 0.33 +0.6 863,786 - CA265_RS03610 0.42 -1.3 863,788 + CA265_RS03610 0.42 -1.0 863,865 + CA265_RS03610 0.49 +1.5 863,866 - -1.1 863,907 + CA265_RS03610 0.53 +0.7 863,987 - CA265_RS03610 0.60 -0.6 864,055 - CA265_RS03610 0.66 +0.1 864,057 + CA265_RS03610 0.66 -0.9 864,168 + CA265_RS03610 0.75 +0.5 864,214 - CA265_RS03610 0.79 -0.1 864,300 + CA265_RS03610 0.87 -1.4 864,313 - CA265_RS03610 0.88 -0.4 864,352 - +0.1 864,490 + -0.8 864,584 + -0.2 864,631 - CA265_RS03615 0.11 -0.9 864,633 + CA265_RS03615 0.11 -1.4 864,633 + CA265_RS03615 0.11 -1.0 864,708 - CA265_RS03615 0.21 -1.1 864,782 + CA265_RS03615 0.31 +1.1 864,788 + CA265_RS03615 0.32 -1.1 864,789 - CA265_RS03615 0.32 +0.9 864,792 + CA265_RS03615 0.32 -0.5 864,879 + CA265_RS03615 0.44 -1.0 864,915 - CA265_RS03615 0.49 -3.0 864,992 + CA265_RS03615 0.59 -1.1 865,044 + CA265_RS03615 0.66 -0.1 865,121 + CA265_RS03615 0.76 -1.6 865,143 + CA265_RS03615 0.79 -1.1 865,154 + CA265_RS03615 0.81 -1.0 865,154 + CA265_RS03615 0.81 -0.8 865,154 + CA265_RS03615 0.81 -1.4 865,154 + CA265_RS03615 0.81 -0.8 865,184 - CA265_RS03615 0.85 +0.9 865,216 + CA265_RS03615 0.89 -0.0 865,281 - +0.5 865,290 + -0.9 865,291 - -1.1 865,291 - -2.3 865,291 - -1.0 865,295 - -0.6 865,302 - +0.3 865,302 - -0.4 865,454 + CA265_RS03620 0.24 +0.5 865,461 + CA265_RS03620 0.25 -0.5 865,481 + CA265_RS03620 0.29 -0.4 865,482 - CA265_RS03620 0.29 -1.1 865,491 + CA265_RS03620 0.30 -1.3 865,494 + CA265_RS03620 0.31 -1.4 865,494 + CA265_RS03620 0.31 -0.6 865,513 + CA265_RS03620 0.34 -0.1 865,612 + CA265_RS03620 0.49 -0.7 865,807 + CA265_RS03620 0.80 -0.7 865,817 + CA265_RS03620 0.82 -0.5 865,837 + CA265_RS03620 0.85 -0.2 865,838 - CA265_RS03620 0.85 -0.8 865,942 + +0.2 865,944 + +0.7 865,954 - -0.2 866,001 - -0.9 866,134 + CA265_RS03625 0.12 +0.2 866,157 + CA265_RS03625 0.14 +0.5 866,178 - CA265_RS03625 0.16 -0.5
Or see this region's nucleotide sequence