Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00645 and CA265_RS00650 are separated by 344 nucleotides CA265_RS00650 and CA265_RS00655 are separated by 88 nucleotides CA265_RS00655 and CA265_RS00660 are separated by 258 nucleotides
CA265_RS00645: CA265_RS00645 - MFS transporter, at 146,261 to 147,445
_RS00645
CA265_RS00650: CA265_RS00650 - hypothetical protein, at 147,790 to 147,984
_RS00650
CA265_RS00655: CA265_RS00655 - RNA polymerase subunit sigma-24, at 148,073 to 148,657
_RS00655
CA265_RS00660: CA265_RS00660 - uvrA paralog in Bacteroidetes <small>(from data)</small>, at 148,916 to 151,729
_RS00660
Position (kb) 147
148 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 146.790 kb on + strand, within CA265_RS00645 at 146.912 kb on + strand, within CA265_RS00645 at 146.918 kb on + strand, within CA265_RS00645 at 146.931 kb on - strand, within CA265_RS00645 at 147.160 kb on + strand, within CA265_RS00645 at 147.257 kb on - strand, within CA265_RS00645 at 147.407 kb on + strand at 147.495 kb on + strand at 147.522 kb on + strand at 147.529 kb on - strand at 147.532 kb on + strand at 147.532 kb on + strand at 147.533 kb on - strand at 147.533 kb on - strand at 147.582 kb on - strand at 147.705 kb on + strand at 147.706 kb on - strand at 147.708 kb on + strand at 147.709 kb on - strand at 147.727 kb on - strand at 147.799 kb on - strand at 147.807 kb on - strand at 147.812 kb on - strand, within CA265_RS00650 at 147.864 kb on + strand, within CA265_RS00650 at 147.881 kb on + strand, within CA265_RS00650 at 147.893 kb on - strand, within CA265_RS00650 at 147.936 kb on + strand, within CA265_RS00650 at 147.970 kb on - strand at 148.008 kb on + strand at 148.017 kb on - strand at 148.023 kb on - strand at 148.146 kb on - strand, within CA265_RS00655 at 148.196 kb on + strand, within CA265_RS00655 at 148.206 kb on + strand, within CA265_RS00655 at 148.273 kb on + strand, within CA265_RS00655 at 148.297 kb on + strand, within CA265_RS00655 at 148.455 kb on + strand, within CA265_RS00655 at 148.477 kb on + strand, within CA265_RS00655 at 148.482 kb on - strand, within CA265_RS00655 at 148.522 kb on - strand, within CA265_RS00655 at 148.546 kb on + strand, within CA265_RS00655 at 148.548 kb on - strand, within CA265_RS00655 at 148.562 kb on - strand, within CA265_RS00655 at 148.567 kb on + strand, within CA265_RS00655 at 148.568 kb on - strand, within CA265_RS00655 at 148.577 kb on + strand, within CA265_RS00655 at 148.612 kb on + strand at 148.613 kb on - strand at 148.613 kb on - strand at 148.614 kb on + strand at 148.615 kb on - strand at 148.641 kb on + strand at 148.642 kb on - strand at 148.642 kb on - strand at 148.664 kb on - strand at 148.684 kb on + strand at 148.698 kb on + strand at 148.705 kb on + strand at 148.867 kb on + strand at 148.894 kb on + strand at 148.899 kb on + strand at 148.913 kb on + strand at 148.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 146,790 + CA265_RS00645 0.45 -0.3 146,912 + CA265_RS00645 0.55 -0.6 146,918 + CA265_RS00645 0.55 -0.1 146,931 - CA265_RS00645 0.57 +0.8 147,160 + CA265_RS00645 0.76 +0.3 147,257 - CA265_RS00645 0.84 +1.6 147,407 + -0.0 147,495 + +0.5 147,522 + +0.1 147,529 - +0.3 147,532 + -0.3 147,532 + +0.1 147,533 - -0.5 147,533 - -1.2 147,582 - -0.0 147,705 + -0.5 147,706 - -0.1 147,708 + +0.6 147,709 - -0.6 147,727 - +0.5 147,799 - +0.7 147,807 - -4.2 147,812 - CA265_RS00650 0.11 -1.8 147,864 + CA265_RS00650 0.38 -1.2 147,881 + CA265_RS00650 0.47 -2.2 147,893 - CA265_RS00650 0.53 -1.5 147,936 + CA265_RS00650 0.75 +0.1 147,970 - -2.1 148,008 + -0.2 148,017 - -0.1 148,023 - +0.8 148,146 - CA265_RS00655 0.12 +0.9 148,196 + CA265_RS00655 0.21 +0.9 148,206 + CA265_RS00655 0.23 +1.3 148,273 + CA265_RS00655 0.34 +0.7 148,297 + CA265_RS00655 0.38 +0.4 148,455 + CA265_RS00655 0.65 +1.1 148,477 + CA265_RS00655 0.69 +0.5 148,482 - CA265_RS00655 0.70 +0.9 148,522 - CA265_RS00655 0.77 -0.3 148,546 + CA265_RS00655 0.81 +1.2 148,548 - CA265_RS00655 0.81 +0.3 148,562 - CA265_RS00655 0.84 +0.1 148,567 + CA265_RS00655 0.84 +1.1 148,568 - CA265_RS00655 0.85 -0.9 148,577 + CA265_RS00655 0.86 +0.7 148,612 + +1.3 148,613 - +0.8 148,613 - +0.6 148,614 + +1.2 148,615 - -1.0 148,641 + +1.3 148,642 - +0.6 148,642 - +0.6 148,664 - -0.8 148,684 + +0.5 148,698 + +0.9 148,705 + +1.6 148,867 + -2.2 148,894 + -3.2 148,899 + -0.2 148,913 + -0.1 148,914 - -1.2
Or see this region's nucleotide sequence