Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2913 and EX28DRAFT_2914 overlap by 40 nucleotides EX28DRAFT_2914 and EX28DRAFT_2915 are separated by 220 nucleotides EX28DRAFT_2915 and EX28DRAFT_2916 overlap by 31 nucleotides
EX28DRAFT_2913: EX28DRAFT_2913 - Transcriptional regulators, at 223,711 to 224,727
_2913
EX28DRAFT_2914: EX28DRAFT_2914 - Acetyltransferase (GNAT) family, at 224,688 to 225,173
_2914
EX28DRAFT_2915: EX28DRAFT_2915 - Transcriptional regulators, at 225,394 to 226,431
_2915
EX28DRAFT_2916: EX28DRAFT_2916 - diaminopimelate decarboxylase, at 226,401 to 227,663
_2916
Position (kb) 225
226
227 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 224.440 kb on + strand, within EX28DRAFT_2913 at 224.776 kb on + strand, within EX28DRAFT_2914 at 224.776 kb on + strand, within EX28DRAFT_2914 at 224.777 kb on - strand, within EX28DRAFT_2914 at 224.777 kb on - strand, within EX28DRAFT_2914 at 224.859 kb on - strand, within EX28DRAFT_2914 at 224.917 kb on + strand, within EX28DRAFT_2914 at 224.918 kb on - strand, within EX28DRAFT_2914 at 224.918 kb on - strand, within EX28DRAFT_2914 at 225.057 kb on - strand, within EX28DRAFT_2914 at 225.146 kb on - strand at 225.196 kb on + strand at 225.197 kb on - strand at 225.197 kb on - strand at 225.233 kb on + strand at 225.252 kb on + strand at 225.253 kb on - strand at 225.262 kb on + strand at 225.336 kb on + strand at 225.357 kb on + strand at 225.372 kb on + strand at 225.373 kb on - strand at 225.390 kb on + strand at 225.391 kb on - strand at 225.391 kb on - strand at 225.391 kb on - strand at 225.486 kb on + strand at 225.610 kb on + strand, within EX28DRAFT_2915 at 225.611 kb on - strand, within EX28DRAFT_2915 at 225.758 kb on - strand, within EX28DRAFT_2915 at 225.767 kb on - strand, within EX28DRAFT_2915 at 225.904 kb on + strand, within EX28DRAFT_2915 at 225.905 kb on - strand, within EX28DRAFT_2915 at 225.940 kb on - strand, within EX28DRAFT_2915 at 225.993 kb on + strand, within EX28DRAFT_2915 at 226.069 kb on + strand, within EX28DRAFT_2915 at 226.076 kb on + strand, within EX28DRAFT_2915 at 226.076 kb on + strand, within EX28DRAFT_2915 at 226.077 kb on - strand, within EX28DRAFT_2915 at 226.195 kb on - strand, within EX28DRAFT_2915 at 226.261 kb on + strand, within EX28DRAFT_2915 at 226.278 kb on + strand, within EX28DRAFT_2915 at 226.279 kb on - strand, within EX28DRAFT_2915 at 226.279 kb on - strand, within EX28DRAFT_2915 at 226.279 kb on - strand, within EX28DRAFT_2915 at 226.394 kb on - strand at 226.508 kb on - strand at 226.508 kb on - strand at 226.512 kb on - strand at 226.558 kb on + strand, within EX28DRAFT_2916 at 226.713 kb on - strand, within EX28DRAFT_2916 at 226.723 kb on + strand, within EX28DRAFT_2916 at 226.732 kb on + strand, within EX28DRAFT_2916 at 227.072 kb on - strand, within EX28DRAFT_2916 at 227.096 kb on + strand, within EX28DRAFT_2916 at 227.131 kb on - strand, within EX28DRAFT_2916 at 227.153 kb on + strand, within EX28DRAFT_2916 at 227.154 kb on - strand, within EX28DRAFT_2916 at 227.154 kb on - strand, within EX28DRAFT_2916 at 227.288 kb on + strand, within EX28DRAFT_2916 at 227.289 kb on - strand, within EX28DRAFT_2916 at 227.292 kb on + strand, within EX28DRAFT_2916 at 227.293 kb on - strand, within EX28DRAFT_2916
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 224,440 + EX28DRAFT_2913 0.72 +0.5 224,776 + EX28DRAFT_2914 0.18 +0.7 224,776 + EX28DRAFT_2914 0.18 -0.4 224,777 - EX28DRAFT_2914 0.18 +0.1 224,777 - EX28DRAFT_2914 0.18 +0.1 224,859 - EX28DRAFT_2914 0.35 +0.5 224,917 + EX28DRAFT_2914 0.47 +1.5 224,918 - EX28DRAFT_2914 0.47 -2.6 224,918 - EX28DRAFT_2914 0.47 +0.1 225,057 - EX28DRAFT_2914 0.76 -0.1 225,146 - -0.4 225,196 + -2.0 225,197 - +0.4 225,197 - -0.8 225,233 + +0.5 225,252 + -3.3 225,253 - -0.8 225,262 + -0.7 225,336 + -0.5 225,357 + -0.3 225,372 + +0.4 225,373 - -0.5 225,390 + +0.4 225,391 - -3.1 225,391 - -1.8 225,391 - -2.1 225,486 + -0.1 225,610 + EX28DRAFT_2915 0.21 +0.1 225,611 - EX28DRAFT_2915 0.21 +1.3 225,758 - EX28DRAFT_2915 0.35 -0.9 225,767 - EX28DRAFT_2915 0.36 -1.8 225,904 + EX28DRAFT_2915 0.49 -0.4 225,905 - EX28DRAFT_2915 0.49 -0.3 225,940 - EX28DRAFT_2915 0.53 -0.6 225,993 + EX28DRAFT_2915 0.58 -1.0 226,069 + EX28DRAFT_2915 0.65 +0.8 226,076 + EX28DRAFT_2915 0.66 +0.7 226,076 + EX28DRAFT_2915 0.66 +0.2 226,077 - EX28DRAFT_2915 0.66 +1.9 226,195 - EX28DRAFT_2915 0.77 +0.4 226,261 + EX28DRAFT_2915 0.84 -0.4 226,278 + EX28DRAFT_2915 0.85 +1.0 226,279 - EX28DRAFT_2915 0.85 +1.5 226,279 - EX28DRAFT_2915 0.85 +0.1 226,279 - EX28DRAFT_2915 0.85 +0.1 226,394 - -0.6 226,508 - -1.8 226,508 - -1.5 226,512 - -1.1 226,558 + EX28DRAFT_2916 0.12 -4.2 226,713 - EX28DRAFT_2916 0.25 -4.1 226,723 + EX28DRAFT_2916 0.25 -3.4 226,732 + EX28DRAFT_2916 0.26 +0.5 227,072 - EX28DRAFT_2916 0.53 -4.6 227,096 + EX28DRAFT_2916 0.55 -3.5 227,131 - EX28DRAFT_2916 0.58 -4.9 227,153 + EX28DRAFT_2916 0.60 -3.7 227,154 - EX28DRAFT_2916 0.60 -2.2 227,154 - EX28DRAFT_2916 0.60 -6.7 227,288 + EX28DRAFT_2916 0.70 -0.3 227,289 - EX28DRAFT_2916 0.70 -6.9 227,292 + EX28DRAFT_2916 0.71 -0.5 227,293 - EX28DRAFT_2916 0.71 -4.8
Or see this region's nucleotide sequence