Experiment: R2A with Tetracycline 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09440 and CA265_RS09445 are separated by 574 nucleotides CA265_RS09445 and CA265_RS09450 are separated by 272 nucleotides
CA265_RS09440: CA265_RS09440 - DNA gyrase subunit A, at 2,337,049 to 2,339,640
_RS09440
CA265_RS09445: CA265_RS09445 - tetracycline resistance MFS efflux pump, at 2,340,215 to 2,341,426
_RS09445
CA265_RS09450: CA265_RS09450 - ribonuclease, at 2,341,699 to 2,343,051
_RS09450
Position (kb)
2340
2341
2342 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2339.662 kb on - strand at 2339.689 kb on - strand at 2339.689 kb on - strand at 2339.691 kb on - strand at 2339.756 kb on + strand at 2339.822 kb on + strand at 2339.858 kb on - strand at 2339.989 kb on - strand at 2340.070 kb on + strand at 2340.177 kb on - strand at 2340.211 kb on + strand at 2340.212 kb on - strand at 2340.304 kb on - strand at 2340.313 kb on + strand at 2340.314 kb on - strand at 2340.314 kb on - strand at 2340.316 kb on - strand at 2340.357 kb on - strand, within CA265_RS09445 at 2340.380 kb on + strand, within CA265_RS09445 at 2340.414 kb on + strand, within CA265_RS09445 at 2340.479 kb on - strand, within CA265_RS09445 at 2340.485 kb on + strand, within CA265_RS09445 at 2340.485 kb on + strand, within CA265_RS09445 at 2340.485 kb on + strand, within CA265_RS09445 at 2340.495 kb on + strand, within CA265_RS09445 at 2340.522 kb on + strand, within CA265_RS09445 at 2340.547 kb on + strand, within CA265_RS09445 at 2340.551 kb on - strand, within CA265_RS09445 at 2340.582 kb on - strand, within CA265_RS09445 at 2340.663 kb on + strand, within CA265_RS09445 at 2340.675 kb on + strand, within CA265_RS09445 at 2340.732 kb on + strand at 2340.738 kb on + strand, within CA265_RS09445 at 2340.752 kb on + strand, within CA265_RS09445 at 2340.753 kb on - strand, within CA265_RS09445 at 2340.763 kb on + strand, within CA265_RS09445 at 2340.764 kb on - strand, within CA265_RS09445 at 2340.867 kb on + strand, within CA265_RS09445 at 2340.896 kb on + strand, within CA265_RS09445 at 2340.936 kb on - strand, within CA265_RS09445 at 2340.984 kb on + strand, within CA265_RS09445 at 2341.027 kb on - strand, within CA265_RS09445 at 2341.087 kb on - strand, within CA265_RS09445 at 2341.087 kb on - strand, within CA265_RS09445 at 2341.212 kb on + strand, within CA265_RS09445 at 2341.257 kb on + strand, within CA265_RS09445 at 2341.358 kb on - strand at 2341.359 kb on + strand at 2341.435 kb on + strand at 2341.435 kb on + strand at 2341.435 kb on + strand at 2341.451 kb on - strand at 2341.530 kb on + strand at 2341.531 kb on - strand at 2341.555 kb on + strand at 2341.556 kb on - strand at 2341.561 kb on - strand at 2341.618 kb on + strand at 2341.618 kb on + strand at 2341.691 kb on + strand at 2341.700 kb on + strand at 2341.755 kb on + strand at 2341.755 kb on + strand at 2341.756 kb on - strand at 2341.766 kb on + strand at 2341.813 kb on - strand at 2341.829 kb on + strand at 2341.830 kb on - strand at 2341.941 kb on + strand, within CA265_RS09450 at 2342.183 kb on + strand, within CA265_RS09450 at 2342.184 kb on - strand at 2342.184 kb on - strand, within CA265_RS09450 at 2342.265 kb on + strand, within CA265_RS09450 at 2342.265 kb on + strand, within CA265_RS09450 at 2342.265 kb on + strand, within CA265_RS09450 at 2342.269 kb on + strand, within CA265_RS09450 at 2342.270 kb on - strand, within CA265_RS09450 at 2342.356 kb on - strand, within CA265_RS09450 at 2342.356 kb on - strand, within CA265_RS09450 at 2342.356 kb on - strand, within CA265_RS09450 at 2342.378 kb on - strand, within CA265_RS09450 at 2342.378 kb on - strand, within CA265_RS09450 at 2342.382 kb on + strand, within CA265_RS09450 at 2342.390 kb on + strand, within CA265_RS09450 at 2342.391 kb on - strand, within CA265_RS09450
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.0005 mg/ml remove 2,339,662 - -0.2 2,339,689 - -0.1 2,339,689 - +0.1 2,339,691 - +0.0 2,339,756 + -0.1 2,339,822 + -1.7 2,339,858 - +0.9 2,339,989 - +0.1 2,340,070 + -0.8 2,340,177 - -0.0 2,340,211 + -1.3 2,340,212 - -2.9 2,340,304 - -2.4 2,340,313 + -2.5 2,340,314 - -2.4 2,340,314 - -2.1 2,340,316 - -1.9 2,340,357 - CA265_RS09445 0.12 -3.3 2,340,380 + CA265_RS09445 0.14 -3.4 2,340,414 + CA265_RS09445 0.16 -1.5 2,340,479 - CA265_RS09445 0.22 -2.7 2,340,485 + CA265_RS09445 0.22 -2.8 2,340,485 + CA265_RS09445 0.22 -3.0 2,340,485 + CA265_RS09445 0.22 -0.0 2,340,495 + CA265_RS09445 0.23 -2.3 2,340,522 + CA265_RS09445 0.25 -2.4 2,340,547 + CA265_RS09445 0.27 -1.2 2,340,551 - CA265_RS09445 0.28 -2.3 2,340,582 - CA265_RS09445 0.30 -1.8 2,340,663 + CA265_RS09445 0.37 -1.3 2,340,675 + CA265_RS09445 0.38 -2.7 2,340,732 + -0.8 2,340,738 + CA265_RS09445 0.43 -2.2 2,340,752 + CA265_RS09445 0.44 +0.1 2,340,753 - CA265_RS09445 0.44 -0.9 2,340,763 + CA265_RS09445 0.45 -2.9 2,340,764 - CA265_RS09445 0.45 -3.0 2,340,867 + CA265_RS09445 0.54 -0.8 2,340,896 + CA265_RS09445 0.56 -1.3 2,340,936 - CA265_RS09445 0.59 -1.8 2,340,984 + CA265_RS09445 0.63 -2.2 2,341,027 - CA265_RS09445 0.67 -3.5 2,341,087 - CA265_RS09445 0.72 -1.5 2,341,087 - CA265_RS09445 0.72 -1.7 2,341,212 + CA265_RS09445 0.82 -2.9 2,341,257 + CA265_RS09445 0.86 -2.4 2,341,358 - -4.5 2,341,359 + -1.9 2,341,435 + +0.4 2,341,435 + +0.2 2,341,435 + +0.1 2,341,451 - -2.3 2,341,530 + -1.0 2,341,531 - -1.2 2,341,555 + -0.4 2,341,556 - -2.1 2,341,561 - -2.2 2,341,618 + +0.3 2,341,618 + +0.0 2,341,691 + +0.1 2,341,700 + +0.3 2,341,755 + +0.5 2,341,755 + +1.1 2,341,756 - -2.5 2,341,766 + -0.2 2,341,813 - -1.0 2,341,829 + +0.2 2,341,830 - -2.1 2,341,941 + CA265_RS09450 0.18 -0.8 2,342,183 + CA265_RS09450 0.36 +0.2 2,342,184 - -0.8 2,342,184 - CA265_RS09450 0.36 +1.1 2,342,265 + CA265_RS09450 0.42 +0.4 2,342,265 + CA265_RS09450 0.42 +0.2 2,342,265 + CA265_RS09450 0.42 +0.1 2,342,269 + CA265_RS09450 0.42 -0.1 2,342,270 - CA265_RS09450 0.42 -2.1 2,342,356 - CA265_RS09450 0.49 -1.3 2,342,356 - CA265_RS09450 0.49 -0.1 2,342,356 - CA265_RS09450 0.49 -0.7 2,342,378 - CA265_RS09450 0.50 -1.5 2,342,378 - CA265_RS09450 0.50 -1.1 2,342,382 + CA265_RS09450 0.50 +0.8 2,342,390 + CA265_RS09450 0.51 -0.5 2,342,391 - CA265_RS09450 0.51 +0.4
Or see this region's nucleotide sequence