Experiment: R2A with Tetracycline 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05345 and CA265_RS05350 are separated by 28 nucleotides CA265_RS05350 and CA265_RS05355 are separated by 34 nucleotides
CA265_RS05345: CA265_RS05345 - hypothetical protein, at 1,282,041 to 1,283,486
_RS05345
CA265_RS05350: CA265_RS05350 - hypothetical protein, at 1,283,515 to 1,284,843
_RS05350
CA265_RS05355: CA265_RS05355 - RagB/SusD family nutrient uptake outer membrane protein, at 1,284,878 to 1,286,707
_RS05355
Position (kb)
1283
1284
1285 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1282.532 kb on + strand, within CA265_RS05345 at 1282.533 kb on - strand, within CA265_RS05345 at 1282.618 kb on + strand, within CA265_RS05345 at 1282.720 kb on + strand, within CA265_RS05345 at 1282.734 kb on - strand, within CA265_RS05345 at 1282.734 kb on - strand, within CA265_RS05345 at 1282.772 kb on + strand, within CA265_RS05345 at 1282.773 kb on - strand, within CA265_RS05345 at 1282.802 kb on + strand, within CA265_RS05345 at 1282.802 kb on + strand, within CA265_RS05345 at 1282.803 kb on - strand, within CA265_RS05345 at 1282.938 kb on + strand, within CA265_RS05345 at 1282.939 kb on - strand, within CA265_RS05345 at 1282.977 kb on - strand, within CA265_RS05345 at 1282.983 kb on - strand, within CA265_RS05345 at 1283.015 kb on + strand, within CA265_RS05345 at 1283.015 kb on + strand, within CA265_RS05345 at 1283.016 kb on - strand, within CA265_RS05345 at 1283.016 kb on - strand, within CA265_RS05345 at 1283.016 kb on - strand, within CA265_RS05345 at 1283.124 kb on - strand, within CA265_RS05345 at 1283.124 kb on - strand, within CA265_RS05345 at 1283.129 kb on + strand, within CA265_RS05345 at 1283.130 kb on - strand, within CA265_RS05345 at 1283.133 kb on + strand, within CA265_RS05345 at 1283.278 kb on - strand, within CA265_RS05345 at 1283.374 kb on - strand at 1283.377 kb on + strand at 1283.378 kb on - strand at 1283.411 kb on + strand at 1283.426 kb on + strand at 1283.455 kb on - strand at 1283.460 kb on + strand at 1283.461 kb on - strand at 1283.488 kb on + strand at 1283.488 kb on + strand at 1283.488 kb on + strand at 1283.511 kb on + strand at 1283.585 kb on - strand at 1283.682 kb on + strand, within CA265_RS05350 at 1283.683 kb on - strand, within CA265_RS05350 at 1283.704 kb on - strand, within CA265_RS05350 at 1283.756 kb on + strand, within CA265_RS05350 at 1283.760 kb on + strand, within CA265_RS05350 at 1283.772 kb on + strand, within CA265_RS05350 at 1283.773 kb on - strand, within CA265_RS05350 at 1283.773 kb on - strand, within CA265_RS05350 at 1283.773 kb on - strand, within CA265_RS05350 at 1283.827 kb on + strand, within CA265_RS05350 at 1283.836 kb on + strand, within CA265_RS05350 at 1283.837 kb on - strand, within CA265_RS05350 at 1283.867 kb on - strand, within CA265_RS05350 at 1283.887 kb on - strand, within CA265_RS05350 at 1283.967 kb on + strand, within CA265_RS05350 at 1284.044 kb on + strand, within CA265_RS05350 at 1284.044 kb on + strand, within CA265_RS05350 at 1284.170 kb on + strand, within CA265_RS05350 at 1284.170 kb on + strand, within CA265_RS05350 at 1284.171 kb on - strand, within CA265_RS05350 at 1284.172 kb on + strand, within CA265_RS05350 at 1284.172 kb on + strand, within CA265_RS05350 at 1284.172 kb on + strand, within CA265_RS05350 at 1284.173 kb on - strand, within CA265_RS05350 at 1284.173 kb on - strand, within CA265_RS05350 at 1284.235 kb on - strand, within CA265_RS05350 at 1284.335 kb on + strand, within CA265_RS05350 at 1284.336 kb on - strand, within CA265_RS05350 at 1284.336 kb on - strand, within CA265_RS05350 at 1284.340 kb on - strand, within CA265_RS05350 at 1284.475 kb on - strand, within CA265_RS05350 at 1284.476 kb on + strand, within CA265_RS05350 at 1284.487 kb on - strand, within CA265_RS05350 at 1284.520 kb on + strand, within CA265_RS05350 at 1284.521 kb on - strand, within CA265_RS05350 at 1284.521 kb on - strand, within CA265_RS05350 at 1284.565 kb on + strand, within CA265_RS05350 at 1284.617 kb on - strand, within CA265_RS05350 at 1284.671 kb on - strand, within CA265_RS05350 at 1284.702 kb on + strand, within CA265_RS05350 at 1284.736 kb on - strand at 1284.792 kb on + strand at 1284.793 kb on - strand at 1284.795 kb on - strand at 1284.799 kb on + strand at 1284.825 kb on + strand at 1284.865 kb on + strand at 1284.865 kb on + strand at 1284.879 kb on + strand at 1284.924 kb on + strand at 1284.945 kb on + strand at 1285.048 kb on - strand at 1285.048 kb on - strand at 1285.051 kb on + strand at 1285.079 kb on + strand, within CA265_RS05355 at 1285.099 kb on - strand, within CA265_RS05355 at 1285.128 kb on - strand, within CA265_RS05355 at 1285.149 kb on + strand, within CA265_RS05355 at 1285.183 kb on + strand, within CA265_RS05355 at 1285.190 kb on + strand, within CA265_RS05355 at 1285.270 kb on - strand, within CA265_RS05355 at 1285.270 kb on - strand, within CA265_RS05355 at 1285.392 kb on + strand, within CA265_RS05355 at 1285.405 kb on + strand, within CA265_RS05355 at 1285.406 kb on - strand, within CA265_RS05355 at 1285.469 kb on - strand, within CA265_RS05355 at 1285.476 kb on - strand, within CA265_RS05355 at 1285.476 kb on - strand, within CA265_RS05355 at 1285.546 kb on - strand, within CA265_RS05355 at 1285.552 kb on + strand, within CA265_RS05355 at 1285.552 kb on + strand, within CA265_RS05355 at 1285.554 kb on + strand, within CA265_RS05355 at 1285.554 kb on + strand, within CA265_RS05355 at 1285.554 kb on + strand, within CA265_RS05355 at 1285.584 kb on + strand, within CA265_RS05355 at 1285.584 kb on + strand, within CA265_RS05355 at 1285.585 kb on - strand, within CA265_RS05355 at 1285.585 kb on - strand, within CA265_RS05355 at 1285.585 kb on - strand, within CA265_RS05355 at 1285.589 kb on - strand, within CA265_RS05355 at 1285.654 kb on + strand, within CA265_RS05355 at 1285.657 kb on - strand, within CA265_RS05355 at 1285.732 kb on - strand, within CA265_RS05355 at 1285.754 kb on - strand, within CA265_RS05355 at 1285.773 kb on + strand, within CA265_RS05355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.0005 mg/ml remove 1,282,532 + CA265_RS05345 0.34 +0.0 1,282,533 - CA265_RS05345 0.34 -1.8 1,282,618 + CA265_RS05345 0.40 +0.0 1,282,720 + CA265_RS05345 0.47 +0.7 1,282,734 - CA265_RS05345 0.48 -1.1 1,282,734 - CA265_RS05345 0.48 -1.0 1,282,772 + CA265_RS05345 0.51 +1.2 1,282,773 - CA265_RS05345 0.51 +0.1 1,282,802 + CA265_RS05345 0.53 -0.5 1,282,802 + CA265_RS05345 0.53 +0.1 1,282,803 - CA265_RS05345 0.53 -1.7 1,282,938 + CA265_RS05345 0.62 +1.1 1,282,939 - CA265_RS05345 0.62 -0.1 1,282,977 - CA265_RS05345 0.65 -0.3 1,282,983 - CA265_RS05345 0.65 +0.8 1,283,015 + CA265_RS05345 0.67 -0.3 1,283,015 + CA265_RS05345 0.67 -0.4 1,283,016 - CA265_RS05345 0.67 -0.9 1,283,016 - CA265_RS05345 0.67 -0.3 1,283,016 - CA265_RS05345 0.67 +0.7 1,283,124 - CA265_RS05345 0.75 +1.0 1,283,124 - CA265_RS05345 0.75 +0.4 1,283,129 + CA265_RS05345 0.75 +0.0 1,283,130 - CA265_RS05345 0.75 -0.1 1,283,133 + CA265_RS05345 0.76 +1.4 1,283,278 - CA265_RS05345 0.86 -0.1 1,283,374 - -0.1 1,283,377 + -0.2 1,283,378 - -0.4 1,283,411 + +0.2 1,283,426 + +1.1 1,283,455 - -0.4 1,283,460 + -0.0 1,283,461 - -0.6 1,283,488 + +1.1 1,283,488 + +1.1 1,283,488 + -0.2 1,283,511 + +0.5 1,283,585 - -0.7 1,283,682 + CA265_RS05350 0.13 +0.8 1,283,683 - CA265_RS05350 0.13 -2.0 1,283,704 - CA265_RS05350 0.14 -1.0 1,283,756 + CA265_RS05350 0.18 -0.9 1,283,760 + CA265_RS05350 0.18 +0.2 1,283,772 + CA265_RS05350 0.19 +0.2 1,283,773 - CA265_RS05350 0.19 -1.7 1,283,773 - CA265_RS05350 0.19 -1.0 1,283,773 - CA265_RS05350 0.19 -2.2 1,283,827 + CA265_RS05350 0.23 -0.7 1,283,836 + CA265_RS05350 0.24 +1.0 1,283,837 - CA265_RS05350 0.24 -2.7 1,283,867 - CA265_RS05350 0.26 -1.3 1,283,887 - CA265_RS05350 0.28 -2.5 1,283,967 + CA265_RS05350 0.34 -0.1 1,284,044 + CA265_RS05350 0.40 -0.1 1,284,044 + CA265_RS05350 0.40 +0.0 1,284,170 + CA265_RS05350 0.49 -0.8 1,284,170 + CA265_RS05350 0.49 -1.4 1,284,171 - CA265_RS05350 0.49 -1.4 1,284,172 + CA265_RS05350 0.49 -1.6 1,284,172 + CA265_RS05350 0.49 +1.0 1,284,172 + CA265_RS05350 0.49 -0.7 1,284,173 - CA265_RS05350 0.50 -1.3 1,284,173 - CA265_RS05350 0.50 -1.5 1,284,235 - CA265_RS05350 0.54 -1.0 1,284,335 + CA265_RS05350 0.62 +1.0 1,284,336 - CA265_RS05350 0.62 -0.3 1,284,336 - CA265_RS05350 0.62 -0.9 1,284,340 - CA265_RS05350 0.62 -0.7 1,284,475 - CA265_RS05350 0.72 +0.3 1,284,476 + CA265_RS05350 0.72 -0.4 1,284,487 - CA265_RS05350 0.73 -1.3 1,284,520 + CA265_RS05350 0.76 +0.7 1,284,521 - CA265_RS05350 0.76 -0.9 1,284,521 - CA265_RS05350 0.76 -1.6 1,284,565 + CA265_RS05350 0.79 -0.0 1,284,617 - CA265_RS05350 0.83 -2.8 1,284,671 - CA265_RS05350 0.87 -0.8 1,284,702 + CA265_RS05350 0.89 +0.1 1,284,736 - -0.9 1,284,792 + +0.9 1,284,793 - -0.9 1,284,795 - -0.7 1,284,799 + +0.7 1,284,825 + +0.2 1,284,865 + +0.2 1,284,865 + +0.5 1,284,879 + -0.0 1,284,924 + +0.4 1,284,945 + +0.6 1,285,048 - -2.8 1,285,048 - -2.1 1,285,051 + -0.3 1,285,079 + CA265_RS05355 0.11 +0.6 1,285,099 - CA265_RS05355 0.12 -1.4 1,285,128 - CA265_RS05355 0.14 -1.5 1,285,149 + CA265_RS05355 0.15 +0.7 1,285,183 + CA265_RS05355 0.17 +0.2 1,285,190 + CA265_RS05355 0.17 -0.1 1,285,270 - CA265_RS05355 0.21 -2.6 1,285,270 - CA265_RS05355 0.21 -1.2 1,285,392 + CA265_RS05355 0.28 -0.3 1,285,405 + CA265_RS05355 0.29 -0.8 1,285,406 - CA265_RS05355 0.29 -2.0 1,285,469 - CA265_RS05355 0.32 -0.2 1,285,476 - CA265_RS05355 0.33 -0.8 1,285,476 - CA265_RS05355 0.33 -2.6 1,285,546 - CA265_RS05355 0.37 -1.3 1,285,552 + CA265_RS05355 0.37 +0.2 1,285,552 + CA265_RS05355 0.37 -0.3 1,285,554 + CA265_RS05355 0.37 -0.2 1,285,554 + CA265_RS05355 0.37 +0.6 1,285,554 + CA265_RS05355 0.37 -0.8 1,285,584 + CA265_RS05355 0.39 +0.1 1,285,584 + CA265_RS05355 0.39 -0.4 1,285,585 - CA265_RS05355 0.39 -1.0 1,285,585 - CA265_RS05355 0.39 -1.3 1,285,585 - CA265_RS05355 0.39 -1.0 1,285,589 - CA265_RS05355 0.39 -1.1 1,285,654 + CA265_RS05355 0.42 -0.3 1,285,657 - CA265_RS05355 0.43 +0.2 1,285,732 - CA265_RS05355 0.47 -0.6 1,285,754 - CA265_RS05355 0.48 -0.6 1,285,773 + CA265_RS05355 0.49 -0.3
Or see this region's nucleotide sequence