Experiment: R2A with Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04300 and CA265_RS04305 are separated by 73 nucleotides CA265_RS04305 and CA265_RS04310 are separated by 126 nucleotides CA265_RS04310 and CA265_RS04315 are separated by 96 nucleotides CA265_RS04315 and CA265_RS04320 are separated by 11 nucleotides
CA265_RS04300: CA265_RS04300 - succinate--CoA ligase subunit beta, at 1,039,376 to 1,040,575
_RS04300
CA265_RS04305: CA265_RS04305 - hypothetical protein, at 1,040,649 to 1,040,885
_RS04305
CA265_RS04310: CA265_RS04310 - hypothetical protein, at 1,041,012 to 1,041,320
_RS04310
CA265_RS04315: CA265_RS04315 - hypothetical protein, at 1,041,417 to 1,041,836
_RS04315
CA265_RS04320: CA265_RS04320 - hypothetical protein, at 1,041,848 to 1,043,416
_RS04320
Position (kb)
1040
1041 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1039.987 kb on - strand, within CA265_RS04300 at 1040.179 kb on + strand, within CA265_RS04300 at 1040.614 kb on - strand at 1040.617 kb on - strand at 1040.742 kb on - strand, within CA265_RS04305 at 1040.753 kb on + strand, within CA265_RS04305 at 1040.762 kb on + strand, within CA265_RS04305 at 1040.779 kb on + strand, within CA265_RS04305 at 1040.876 kb on - strand at 1041.000 kb on + strand at 1041.003 kb on + strand at 1041.041 kb on + strand at 1041.073 kb on - strand, within CA265_RS04310 at 1041.096 kb on + strand, within CA265_RS04310 at 1041.096 kb on + strand, within CA265_RS04310 at 1041.097 kb on - strand, within CA265_RS04310 at 1041.159 kb on - strand, within CA265_RS04310 at 1041.169 kb on - strand, within CA265_RS04310 at 1041.174 kb on - strand, within CA265_RS04310 at 1041.218 kb on + strand, within CA265_RS04310 at 1041.278 kb on + strand, within CA265_RS04310 at 1041.334 kb on - strand at 1041.334 kb on - strand at 1041.334 kb on - strand at 1041.353 kb on + strand at 1041.418 kb on + strand at 1041.505 kb on + strand, within CA265_RS04315 at 1041.561 kb on + strand, within CA265_RS04315 at 1041.561 kb on + strand, within CA265_RS04315 at 1041.562 kb on - strand, within CA265_RS04315 at 1041.609 kb on + strand, within CA265_RS04315 at 1041.650 kb on + strand, within CA265_RS04315 at 1041.651 kb on - strand, within CA265_RS04315 at 1041.672 kb on - strand, within CA265_RS04315 at 1041.673 kb on + strand, within CA265_RS04315 at 1041.735 kb on + strand, within CA265_RS04315 at 1041.739 kb on - strand, within CA265_RS04315 at 1041.740 kb on + strand, within CA265_RS04315 at 1041.740 kb on + strand, within CA265_RS04315 at 1041.740 kb on + strand, within CA265_RS04315 at 1041.741 kb on - strand, within CA265_RS04315 at 1041.750 kb on + strand, within CA265_RS04315 at 1041.762 kb on - strand, within CA265_RS04315 at 1041.867 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.001 mg/ml remove 1,039,987 - CA265_RS04300 0.51 +0.3 1,040,179 + CA265_RS04300 0.67 -1.0 1,040,614 - +0.1 1,040,617 - -0.7 1,040,742 - CA265_RS04305 0.39 -0.7 1,040,753 + CA265_RS04305 0.44 +1.8 1,040,762 + CA265_RS04305 0.48 +0.0 1,040,779 + CA265_RS04305 0.55 -1.8 1,040,876 - +0.2 1,041,000 + +0.3 1,041,003 + +0.6 1,041,041 + -0.0 1,041,073 - CA265_RS04310 0.20 -1.0 1,041,096 + CA265_RS04310 0.27 +0.2 1,041,096 + CA265_RS04310 0.27 -0.6 1,041,097 - CA265_RS04310 0.28 -1.6 1,041,159 - CA265_RS04310 0.48 -0.2 1,041,169 - CA265_RS04310 0.51 +0.5 1,041,174 - CA265_RS04310 0.52 +1.3 1,041,218 + CA265_RS04310 0.67 +0.1 1,041,278 + CA265_RS04310 0.86 -0.1 1,041,334 - -0.2 1,041,334 - -0.1 1,041,334 - +0.4 1,041,353 + -0.9 1,041,418 + -0.2 1,041,505 + CA265_RS04315 0.21 +0.8 1,041,561 + CA265_RS04315 0.34 +0.1 1,041,561 + CA265_RS04315 0.34 -0.7 1,041,562 - CA265_RS04315 0.35 -1.3 1,041,609 + CA265_RS04315 0.46 -0.7 1,041,650 + CA265_RS04315 0.55 -0.2 1,041,651 - CA265_RS04315 0.56 +0.5 1,041,672 - CA265_RS04315 0.61 +0.4 1,041,673 + CA265_RS04315 0.61 -0.7 1,041,735 + CA265_RS04315 0.76 -0.5 1,041,739 - CA265_RS04315 0.77 +0.8 1,041,740 + CA265_RS04315 0.77 +0.5 1,041,740 + CA265_RS04315 0.77 +0.1 1,041,740 + CA265_RS04315 0.77 +1.3 1,041,741 - CA265_RS04315 0.77 +0.0 1,041,750 + CA265_RS04315 0.79 -0.3 1,041,762 - CA265_RS04315 0.82 +0.4 1,041,867 + -0.6
Or see this region's nucleotide sequence