Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4048 and EX28DRAFT_4049 are separated by 159 nucleotides EX28DRAFT_4049 and EX28DRAFT_4050 are separated by 227 nucleotides
EX28DRAFT_4048: EX28DRAFT_4048 - FOG: EAL domain, at 17,752 to 19,758
_4048
EX28DRAFT_4049: EX28DRAFT_4049 - Na+/H+-dicarboxylate symporters, at 19,918 to 21,204
_4049
EX28DRAFT_4050: EX28DRAFT_4050 - Predicted Zn-dependent peptidases, at 21,432 to 22,925
_4050
Position (kb)
19
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1 at 19.125 kb on - strand, within EX28DRAFT_4048 at 19.275 kb on + strand, within EX28DRAFT_4048 at 19.339 kb on - strand, within EX28DRAFT_4048 at 19.339 kb on - strand, within EX28DRAFT_4048 at 19.344 kb on - strand, within EX28DRAFT_4048 at 19.605 kb on + strand at 19.606 kb on - strand at 19.841 kb on + strand at 19.842 kb on - strand at 19.883 kb on - strand at 19.917 kb on + strand at 19.917 kb on + strand at 19.945 kb on + strand at 19.945 kb on + strand at 19.994 kb on - strand at 19.996 kb on + strand at 20.237 kb on + strand, within EX28DRAFT_4049 at 20.238 kb on - strand, within EX28DRAFT_4049 at 20.436 kb on + strand, within EX28DRAFT_4049 at 20.437 kb on - strand, within EX28DRAFT_4049 at 20.442 kb on + strand, within EX28DRAFT_4049 at 20.443 kb on - strand, within EX28DRAFT_4049 at 20.488 kb on - strand, within EX28DRAFT_4049 at 20.535 kb on + strand, within EX28DRAFT_4049 at 20.544 kb on + strand, within EX28DRAFT_4049 at 20.544 kb on + strand, within EX28DRAFT_4049 at 20.544 kb on + strand, within EX28DRAFT_4049 at 20.545 kb on - strand, within EX28DRAFT_4049 at 20.545 kb on - strand, within EX28DRAFT_4049 at 20.548 kb on + strand, within EX28DRAFT_4049 at 20.548 kb on + strand, within EX28DRAFT_4049 at 20.587 kb on - strand, within EX28DRAFT_4049 at 20.596 kb on + strand, within EX28DRAFT_4049 at 20.596 kb on + strand, within EX28DRAFT_4049 at 20.596 kb on + strand, within EX28DRAFT_4049 at 20.596 kb on + strand, within EX28DRAFT_4049 at 20.596 kb on + strand, within EX28DRAFT_4049 at 20.596 kb on + strand, within EX28DRAFT_4049 at 20.597 kb on - strand, within EX28DRAFT_4049 at 20.598 kb on + strand, within EX28DRAFT_4049 at 20.599 kb on - strand, within EX28DRAFT_4049 at 20.680 kb on + strand, within EX28DRAFT_4049 at 20.743 kb on - strand, within EX28DRAFT_4049 at 20.839 kb on + strand, within EX28DRAFT_4049 at 20.839 kb on + strand, within EX28DRAFT_4049 at 20.840 kb on - strand, within EX28DRAFT_4049 at 20.840 kb on - strand, within EX28DRAFT_4049 at 20.840 kb on - strand, within EX28DRAFT_4049 at 20.840 kb on - strand, within EX28DRAFT_4049 at 20.887 kb on - strand, within EX28DRAFT_4049 at 20.938 kb on - strand, within EX28DRAFT_4049 at 20.970 kb on + strand, within EX28DRAFT_4049 at 21.153 kb on + strand at 21.162 kb on + strand at 21.163 kb on - strand at 21.194 kb on + strand at 21.194 kb on + strand at 21.255 kb on + strand at 21.258 kb on + strand at 21.259 kb on - strand at 21.269 kb on - strand at 21.303 kb on + strand at 21.303 kb on + strand at 21.304 kb on - strand at 21.599 kb on + strand, within EX28DRAFT_4050 at 21.602 kb on - strand, within EX28DRAFT_4050 at 21.617 kb on + strand, within EX28DRAFT_4050 at 21.617 kb on + strand, within EX28DRAFT_4050 at 21.618 kb on - strand, within EX28DRAFT_4050 at 21.620 kb on + strand, within EX28DRAFT_4050 at 21.621 kb on - strand, within EX28DRAFT_4050 at 21.621 kb on - strand, within EX28DRAFT_4050 at 21.783 kb on + strand, within EX28DRAFT_4050 at 21.784 kb on - strand, within EX28DRAFT_4050 at 21.789 kb on + strand, within EX28DRAFT_4050 at 21.818 kb on + strand, within EX28DRAFT_4050 at 21.849 kb on - strand, within EX28DRAFT_4050 at 21.852 kb on + strand, within EX28DRAFT_4050 at 21.852 kb on + strand, within EX28DRAFT_4050 at 21.853 kb on - strand, within EX28DRAFT_4050 at 21.903 kb on + strand, within EX28DRAFT_4050 at 21.904 kb on - strand, within EX28DRAFT_4050 at 22.045 kb on + strand, within EX28DRAFT_4050 at 22.045 kb on + strand, within EX28DRAFT_4050 at 22.046 kb on - strand, within EX28DRAFT_4050
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 19,125 - EX28DRAFT_4048 0.68 +0.2 19,275 + EX28DRAFT_4048 0.76 +0.2 19,339 - EX28DRAFT_4048 0.79 -0.3 19,339 - EX28DRAFT_4048 0.79 +0.5 19,344 - EX28DRAFT_4048 0.79 -0.2 19,605 + -0.1 19,606 - +0.9 19,841 + -0.1 19,842 - +0.3 19,883 - +0.5 19,917 + -0.1 19,917 + -0.5 19,945 + -0.5 19,945 + -1.8 19,994 - +0.2 19,996 + -0.1 20,237 + EX28DRAFT_4049 0.25 -0.0 20,238 - EX28DRAFT_4049 0.25 -0.5 20,436 + EX28DRAFT_4049 0.40 +0.3 20,437 - EX28DRAFT_4049 0.40 +0.0 20,442 + EX28DRAFT_4049 0.41 +0.1 20,443 - EX28DRAFT_4049 0.41 -0.3 20,488 - EX28DRAFT_4049 0.44 -0.3 20,535 + EX28DRAFT_4049 0.48 -0.1 20,544 + EX28DRAFT_4049 0.49 +0.4 20,544 + EX28DRAFT_4049 0.49 -0.1 20,544 + EX28DRAFT_4049 0.49 +0.2 20,545 - EX28DRAFT_4049 0.49 -1.5 20,545 - EX28DRAFT_4049 0.49 -0.1 20,548 + EX28DRAFT_4049 0.49 -0.7 20,548 + EX28DRAFT_4049 0.49 +0.6 20,587 - EX28DRAFT_4049 0.52 -0.2 20,596 + EX28DRAFT_4049 0.53 -0.4 20,596 + EX28DRAFT_4049 0.53 -0.8 20,596 + EX28DRAFT_4049 0.53 -0.2 20,596 + EX28DRAFT_4049 0.53 +0.0 20,596 + EX28DRAFT_4049 0.53 -1.0 20,596 + EX28DRAFT_4049 0.53 +0.4 20,597 - EX28DRAFT_4049 0.53 +0.1 20,598 + EX28DRAFT_4049 0.53 -0.2 20,599 - EX28DRAFT_4049 0.53 +0.1 20,680 + EX28DRAFT_4049 0.59 +0.8 20,743 - EX28DRAFT_4049 0.64 -1.7 20,839 + EX28DRAFT_4049 0.72 +0.1 20,839 + EX28DRAFT_4049 0.72 -0.1 20,840 - EX28DRAFT_4049 0.72 -0.2 20,840 - EX28DRAFT_4049 0.72 -0.0 20,840 - EX28DRAFT_4049 0.72 -0.3 20,840 - EX28DRAFT_4049 0.72 -0.3 20,887 - EX28DRAFT_4049 0.75 -1.9 20,938 - EX28DRAFT_4049 0.79 +0.1 20,970 + EX28DRAFT_4049 0.82 +0.5 21,153 + -0.2 21,162 + +0.1 21,163 - -1.1 21,194 + +0.3 21,194 + +0.8 21,255 + -0.6 21,258 + -0.3 21,259 - -0.1 21,269 - -1.1 21,303 + -0.1 21,303 + -0.1 21,304 - +0.4 21,599 + EX28DRAFT_4050 0.11 -1.2 21,602 - EX28DRAFT_4050 0.11 +1.0 21,617 + EX28DRAFT_4050 0.12 +0.2 21,617 + EX28DRAFT_4050 0.12 +0.3 21,618 - EX28DRAFT_4050 0.12 +0.6 21,620 + EX28DRAFT_4050 0.13 -0.8 21,621 - EX28DRAFT_4050 0.13 -0.2 21,621 - EX28DRAFT_4050 0.13 -2.1 21,783 + EX28DRAFT_4050 0.23 +0.1 21,784 - EX28DRAFT_4050 0.24 +0.1 21,789 + EX28DRAFT_4050 0.24 -0.8 21,818 + EX28DRAFT_4050 0.26 +0.6 21,849 - EX28DRAFT_4050 0.28 +1.1 21,852 + EX28DRAFT_4050 0.28 +0.8 21,852 + EX28DRAFT_4050 0.28 +0.1 21,853 - EX28DRAFT_4050 0.28 +0.3 21,903 + EX28DRAFT_4050 0.32 -0.1 21,904 - EX28DRAFT_4050 0.32 -0.1 22,045 + EX28DRAFT_4050 0.41 +1.0 22,045 + EX28DRAFT_4050 0.41 -0.0 22,046 - EX28DRAFT_4050 0.41 +0.2
Or see this region's nucleotide sequence