Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4218 and EX28DRAFT_4219 are separated by 49 nucleotides EX28DRAFT_4219 and EX28DRAFT_4220 are separated by 10 nucleotides EX28DRAFT_4220 and EX28DRAFT_4221 overlap by 1 nucleotides
EX28DRAFT_4218: EX28DRAFT_4218 - ABC-type sugar transport system, periplasmic component, at 25,349 to 26,290
_4218
EX28DRAFT_4219: EX28DRAFT_4219 - ABC-type sugar transport system, auxiliary component, at 26,340 to 27,026
_4219
EX28DRAFT_4220: EX28DRAFT_4220 - ketose-bisphosphate aldolase, at 27,037 to 27,897
_4220
EX28DRAFT_4221: EX28DRAFT_4221 - Sugar kinases, ribokinase family, at 27,897 to 28,910
_4221
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 25.343 kb on + strand at 25.343 kb on + strand at 25.345 kb on - strand at 25.346 kb on + strand at 25.346 kb on + strand at 25.346 kb on + strand at 25.346 kb on + strand at 25.346 kb on + strand at 25.347 kb on - strand at 25.347 kb on - strand at 25.347 kb on - strand at 25.348 kb on + strand at 25.348 kb on + strand at 25.348 kb on + strand at 25.349 kb on - strand at 25.768 kb on + strand, within EX28DRAFT_4218 at 25.769 kb on - strand, within EX28DRAFT_4218 at 25.769 kb on - strand, within EX28DRAFT_4218 at 25.769 kb on - strand, within EX28DRAFT_4218 at 25.769 kb on - strand, within EX28DRAFT_4218 at 25.772 kb on + strand, within EX28DRAFT_4218 at 25.772 kb on + strand, within EX28DRAFT_4218 at 25.773 kb on - strand, within EX28DRAFT_4218 at 25.773 kb on - strand, within EX28DRAFT_4218 at 25.949 kb on - strand, within EX28DRAFT_4218 at 26.267 kb on + strand at 26.267 kb on + strand at 26.267 kb on + strand at 26.267 kb on + strand at 26.267 kb on + strand at 26.268 kb on - strand at 26.268 kb on - strand at 26.268 kb on - strand at 26.710 kb on + strand, within EX28DRAFT_4219 at 26.710 kb on + strand, within EX28DRAFT_4219 at 26.710 kb on + strand, within EX28DRAFT_4219 at 26.956 kb on - strand, within EX28DRAFT_4219 at 26.957 kb on + strand, within EX28DRAFT_4219 at 26.957 kb on + strand, within EX28DRAFT_4219 at 26.957 kb on + strand, within EX28DRAFT_4219 at 27.007 kb on - strand at 27.007 kb on - strand at 27.024 kb on + strand at 27.025 kb on - strand at 27.025 kb on - strand at 27.025 kb on - strand at 27.032 kb on - strand at 27.032 kb on - strand at 27.198 kb on + strand, within EX28DRAFT_4220 at 27.199 kb on - strand, within EX28DRAFT_4220 at 27.202 kb on + strand, within EX28DRAFT_4220 at 27.202 kb on + strand, within EX28DRAFT_4220 at 27.202 kb on + strand, within EX28DRAFT_4220 at 27.202 kb on + strand, within EX28DRAFT_4220 at 27.203 kb on - strand, within EX28DRAFT_4220 at 27.355 kb on - strand, within EX28DRAFT_4220 at 27.364 kb on + strand, within EX28DRAFT_4220 at 27.365 kb on - strand, within EX28DRAFT_4220 at 27.586 kb on + strand, within EX28DRAFT_4220 at 27.586 kb on + strand, within EX28DRAFT_4220 at 27.799 kb on + strand, within EX28DRAFT_4220 at 27.800 kb on - strand, within EX28DRAFT_4220 at 27.992 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 25,343 + +0.6 25,343 + +1.9 25,345 - +0.5 25,346 + +1.2 25,346 + +0.9 25,346 + -0.8 25,346 + -2.0 25,346 + -0.2 25,347 - -0.3 25,347 - -0.2 25,347 - +0.1 25,348 + -1.7 25,348 + -0.2 25,348 + +0.7 25,349 - +0.3 25,768 + EX28DRAFT_4218 0.44 +0.4 25,769 - EX28DRAFT_4218 0.45 -0.3 25,769 - EX28DRAFT_4218 0.45 +0.0 25,769 - EX28DRAFT_4218 0.45 +0.1 25,769 - EX28DRAFT_4218 0.45 -0.3 25,772 + EX28DRAFT_4218 0.45 -0.2 25,772 + EX28DRAFT_4218 0.45 -3.0 25,773 - EX28DRAFT_4218 0.45 -0.6 25,773 - EX28DRAFT_4218 0.45 +0.3 25,949 - EX28DRAFT_4218 0.64 +0.4 26,267 + +0.7 26,267 + -0.0 26,267 + +0.2 26,267 + -0.0 26,267 + +1.3 26,268 - -0.6 26,268 - -2.0 26,268 - -0.6 26,710 + EX28DRAFT_4219 0.54 +0.1 26,710 + EX28DRAFT_4219 0.54 +1.8 26,710 + EX28DRAFT_4219 0.54 +1.7 26,956 - EX28DRAFT_4219 0.90 +0.0 26,957 + EX28DRAFT_4219 0.90 +0.8 26,957 + EX28DRAFT_4219 0.90 +0.3 26,957 + EX28DRAFT_4219 0.90 -2.4 27,007 - -2.4 27,007 - +0.7 27,024 + -0.8 27,025 - +0.2 27,025 - -0.0 27,025 - -0.2 27,032 - +0.6 27,032 - -0.6 27,198 + EX28DRAFT_4220 0.19 -0.5 27,199 - EX28DRAFT_4220 0.19 +0.7 27,202 + EX28DRAFT_4220 0.19 +1.8 27,202 + EX28DRAFT_4220 0.19 -0.0 27,202 + EX28DRAFT_4220 0.19 +0.2 27,202 + EX28DRAFT_4220 0.19 -2.0 27,203 - EX28DRAFT_4220 0.19 +0.3 27,355 - EX28DRAFT_4220 0.37 -0.6 27,364 + EX28DRAFT_4220 0.38 +0.4 27,365 - EX28DRAFT_4220 0.38 +0.3 27,586 + EX28DRAFT_4220 0.64 +0.2 27,586 + EX28DRAFT_4220 0.64 +0.2 27,799 + EX28DRAFT_4220 0.89 -0.4 27,800 - EX28DRAFT_4220 0.89 -0.5 27,992 + +2.9
Or see this region's nucleotide sequence