Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3466 and EX28DRAFT_3467 are separated by 2 nucleotides EX28DRAFT_3467 and EX28DRAFT_3468 are separated by 23 nucleotides EX28DRAFT_3468 and EX28DRAFT_3469 are separated by 196 nucleotides EX28DRAFT_3469 and EX28DRAFT_3470 are separated by 10 nucleotides EX28DRAFT_3470 and EX28DRAFT_3471 are separated by 23 nucleotides
EX28DRAFT_3466: EX28DRAFT_3466 - hypothetical protein, at 138,745 to 139,287
_3466
EX28DRAFT_3467: EX28DRAFT_3467 - hypothetical protein, at 139,290 to 139,646
_3467
EX28DRAFT_3468: EX28DRAFT_3468 - YecR-like lipoprotein, at 139,670 to 139,990
_3468
EX28DRAFT_3469: EX28DRAFT_3469 - hypothetical protein, at 140,187 to 140,597
_3469
EX28DRAFT_3470: EX28DRAFT_3470 - hypothetical protein, at 140,608 to 140,898
_3470
EX28DRAFT_3471: EX28DRAFT_3471 - hypothetical protein, at 140,922 to 141,128
_3471
Position (kb)
139
140 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 138.698 kb on + strand at 138.698 kb on + strand at 138.698 kb on + strand at 138.698 kb on + strand at 138.699 kb on - strand at 138.721 kb on + strand at 138.721 kb on + strand at 138.721 kb on + strand at 138.721 kb on + strand at 138.722 kb on - strand at 138.774 kb on - strand at 138.835 kb on + strand, within EX28DRAFT_3466 at 138.835 kb on + strand, within EX28DRAFT_3466 at 138.836 kb on - strand, within EX28DRAFT_3466 at 138.836 kb on - strand, within EX28DRAFT_3466 at 138.836 kb on - strand, within EX28DRAFT_3466 at 138.836 kb on - strand, within EX28DRAFT_3466 at 138.836 kb on - strand, within EX28DRAFT_3466 at 138.866 kb on - strand, within EX28DRAFT_3466 at 138.866 kb on - strand, within EX28DRAFT_3466 at 138.866 kb on - strand, within EX28DRAFT_3466 at 138.869 kb on + strand, within EX28DRAFT_3466 at 138.869 kb on + strand, within EX28DRAFT_3466 at 138.895 kb on + strand, within EX28DRAFT_3466 at 138.940 kb on + strand, within EX28DRAFT_3466 at 138.941 kb on - strand, within EX28DRAFT_3466 at 138.941 kb on - strand, within EX28DRAFT_3466 at 138.941 kb on - strand, within EX28DRAFT_3466 at 138.981 kb on + strand, within EX28DRAFT_3466 at 138.986 kb on - strand, within EX28DRAFT_3466 at 139.059 kb on + strand, within EX28DRAFT_3466 at 139.075 kb on + strand, within EX28DRAFT_3466 at 139.076 kb on - strand, within EX28DRAFT_3466 at 139.077 kb on + strand, within EX28DRAFT_3466 at 139.077 kb on + strand, within EX28DRAFT_3466 at 139.078 kb on - strand, within EX28DRAFT_3466 at 139.078 kb on - strand, within EX28DRAFT_3466 at 139.139 kb on + strand, within EX28DRAFT_3466 at 139.140 kb on - strand, within EX28DRAFT_3466 at 139.176 kb on + strand, within EX28DRAFT_3466 at 139.176 kb on + strand, within EX28DRAFT_3466 at 139.186 kb on + strand, within EX28DRAFT_3466 at 139.216 kb on + strand, within EX28DRAFT_3466 at 139.226 kb on + strand, within EX28DRAFT_3466 at 139.227 kb on - strand, within EX28DRAFT_3466 at 139.227 kb on - strand, within EX28DRAFT_3466 at 139.227 kb on - strand, within EX28DRAFT_3466 at 139.230 kb on + strand, within EX28DRAFT_3466 at 139.230 kb on + strand, within EX28DRAFT_3466 at 139.230 kb on + strand, within EX28DRAFT_3466 at 139.230 kb on + strand, within EX28DRAFT_3466 at 139.231 kb on - strand, within EX28DRAFT_3466 at 139.231 kb on - strand, within EX28DRAFT_3466 at 139.255 kb on + strand at 139.256 kb on - strand at 139.633 kb on - strand at 139.645 kb on - strand at 139.666 kb on + strand at 139.666 kb on + strand at 139.667 kb on - strand at 139.667 kb on - strand at 139.672 kb on - strand at 139.672 kb on - strand at 139.690 kb on + strand at 139.691 kb on - strand at 139.691 kb on - strand at 139.691 kb on - strand at 139.691 kb on - strand at 139.698 kb on + strand at 139.698 kb on + strand at 139.698 kb on + strand at 139.699 kb on - strand at 139.724 kb on - strand, within EX28DRAFT_3468 at 139.724 kb on - strand, within EX28DRAFT_3468 at 139.759 kb on + strand, within EX28DRAFT_3468 at 139.760 kb on - strand, within EX28DRAFT_3468 at 139.760 kb on - strand, within EX28DRAFT_3468 at 139.847 kb on - strand, within EX28DRAFT_3468 at 139.960 kb on + strand at 139.960 kb on + strand at 139.961 kb on - strand at 139.961 kb on - strand at 139.977 kb on - strand at 139.985 kb on - strand at 139.990 kb on + strand at 139.991 kb on - strand at 139.991 kb on - strand at 140.020 kb on + strand at 140.038 kb on - strand at 140.059 kb on + strand at 140.095 kb on + strand at 140.095 kb on + strand at 140.129 kb on - strand at 140.129 kb on - strand at 140.149 kb on - strand at 140.153 kb on + strand at 140.172 kb on + strand at 140.172 kb on + strand at 140.172 kb on + strand at 140.172 kb on + strand at 140.172 kb on + strand at 140.173 kb on - strand at 140.173 kb on - strand at 140.173 kb on - strand at 140.173 kb on - strand at 140.173 kb on - strand at 140.173 kb on - strand at 140.176 kb on + strand at 140.176 kb on + strand at 140.176 kb on + strand at 140.200 kb on + strand at 140.219 kb on + strand at 140.219 kb on + strand at 140.220 kb on - strand at 140.220 kb on - strand at 140.267 kb on + strand, within EX28DRAFT_3469 at 140.308 kb on + strand, within EX28DRAFT_3469 at 140.312 kb on + strand, within EX28DRAFT_3469 at 140.313 kb on - strand, within EX28DRAFT_3469 at 140.317 kb on + strand, within EX28DRAFT_3469 at 140.317 kb on + strand, within EX28DRAFT_3469 at 140.318 kb on - strand, within EX28DRAFT_3469 at 140.433 kb on - strand, within EX28DRAFT_3469 at 140.433 kb on - strand, within EX28DRAFT_3469 at 140.469 kb on - strand, within EX28DRAFT_3469 at 140.548 kb on - strand, within EX28DRAFT_3469 at 140.567 kb on + strand at 140.568 kb on - strand at 140.631 kb on + strand at 140.632 kb on - strand at 140.632 kb on - strand at 140.634 kb on + strand at 140.635 kb on - strand at 140.638 kb on + strand, within EX28DRAFT_3470 at 140.639 kb on - strand, within EX28DRAFT_3470 at 140.640 kb on + strand, within EX28DRAFT_3470 at 140.640 kb on + strand, within EX28DRAFT_3470 at 140.672 kb on - strand, within EX28DRAFT_3470 at 140.708 kb on + strand, within EX28DRAFT_3470 at 140.726 kb on + strand, within EX28DRAFT_3470 at 140.726 kb on + strand, within EX28DRAFT_3470 at 140.727 kb on - strand, within EX28DRAFT_3470 at 140.727 kb on - strand, within EX28DRAFT_3470 at 140.727 kb on - strand, within EX28DRAFT_3470 at 140.727 kb on - strand, within EX28DRAFT_3470 at 140.727 kb on - strand, within EX28DRAFT_3470 at 140.727 kb on - strand, within EX28DRAFT_3470 at 140.727 kb on - strand, within EX28DRAFT_3470 at 140.745 kb on + strand, within EX28DRAFT_3470 at 140.746 kb on - strand, within EX28DRAFT_3470 at 140.763 kb on + strand, within EX28DRAFT_3470 at 140.779 kb on - strand, within EX28DRAFT_3470 at 140.789 kb on + strand, within EX28DRAFT_3470 at 140.790 kb on - strand, within EX28DRAFT_3470 at 140.790 kb on - strand, within EX28DRAFT_3470 at 140.800 kb on - strand, within EX28DRAFT_3470 at 140.802 kb on + strand, within EX28DRAFT_3470 at 140.802 kb on + strand, within EX28DRAFT_3470 at 140.803 kb on - strand, within EX28DRAFT_3470 at 140.831 kb on + strand, within EX28DRAFT_3470 at 140.831 kb on + strand, within EX28DRAFT_3470 at 140.831 kb on + strand, within EX28DRAFT_3470 at 140.831 kb on + strand, within EX28DRAFT_3470 at 140.832 kb on - strand, within EX28DRAFT_3470 at 140.832 kb on - strand, within EX28DRAFT_3470 at 140.836 kb on + strand, within EX28DRAFT_3470 at 140.885 kb on + strand at 140.903 kb on - strand at 140.954 kb on + strand, within EX28DRAFT_3471 at 140.986 kb on + strand, within EX28DRAFT_3471 at 140.987 kb on - strand, within EX28DRAFT_3471 at 140.987 kb on - strand, within EX28DRAFT_3471
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 138,698 + +0.3 138,698 + +0.9 138,698 + +2.4 138,698 + -0.1 138,699 - +0.5 138,721 + +0.2 138,721 + -0.1 138,721 + +0.4 138,721 + +0.8 138,722 - +0.6 138,774 - +0.3 138,835 + EX28DRAFT_3466 0.17 +0.6 138,835 + EX28DRAFT_3466 0.17 -0.3 138,836 - EX28DRAFT_3466 0.17 -0.1 138,836 - EX28DRAFT_3466 0.17 +1.2 138,836 - EX28DRAFT_3466 0.17 +0.9 138,836 - EX28DRAFT_3466 0.17 +1.8 138,836 - EX28DRAFT_3466 0.17 -0.2 138,866 - EX28DRAFT_3466 0.22 -0.5 138,866 - EX28DRAFT_3466 0.22 +0.5 138,866 - EX28DRAFT_3466 0.22 -1.0 138,869 + EX28DRAFT_3466 0.23 +0.6 138,869 + EX28DRAFT_3466 0.23 +0.1 138,895 + EX28DRAFT_3466 0.28 -0.8 138,940 + EX28DRAFT_3466 0.36 -0.5 138,941 - EX28DRAFT_3466 0.36 -0.7 138,941 - EX28DRAFT_3466 0.36 +0.4 138,941 - EX28DRAFT_3466 0.36 -0.6 138,981 + EX28DRAFT_3466 0.43 +0.6 138,986 - EX28DRAFT_3466 0.44 +0.4 139,059 + EX28DRAFT_3466 0.58 -0.0 139,075 + EX28DRAFT_3466 0.61 -2.1 139,076 - EX28DRAFT_3466 0.61 -0.0 139,077 + EX28DRAFT_3466 0.61 +2.5 139,077 + EX28DRAFT_3466 0.61 -0.8 139,078 - EX28DRAFT_3466 0.61 -0.1 139,078 - EX28DRAFT_3466 0.61 +0.1 139,139 + EX28DRAFT_3466 0.73 +0.3 139,140 - EX28DRAFT_3466 0.73 +0.1 139,176 + EX28DRAFT_3466 0.79 +0.8 139,176 + EX28DRAFT_3466 0.79 -0.1 139,186 + EX28DRAFT_3466 0.81 +0.6 139,216 + EX28DRAFT_3466 0.87 +0.5 139,226 + EX28DRAFT_3466 0.89 -0.9 139,227 - EX28DRAFT_3466 0.89 -0.2 139,227 - EX28DRAFT_3466 0.89 +0.3 139,227 - EX28DRAFT_3466 0.89 -3.0 139,230 + EX28DRAFT_3466 0.89 +0.5 139,230 + EX28DRAFT_3466 0.89 +0.3 139,230 + EX28DRAFT_3466 0.89 +0.6 139,230 + EX28DRAFT_3466 0.89 +0.1 139,231 - EX28DRAFT_3466 0.90 -0.3 139,231 - EX28DRAFT_3466 0.90 +0.6 139,255 + -0.2 139,256 - -2.1 139,633 - -1.5 139,645 - -3.8 139,666 + +1.3 139,666 + +0.1 139,667 - -0.2 139,667 - -0.3 139,672 - +0.1 139,672 - -1.0 139,690 + -0.1 139,691 - -0.3 139,691 - +0.9 139,691 - +0.3 139,691 - +0.2 139,698 + -0.3 139,698 + -0.3 139,698 + +0.9 139,699 - +0.8 139,724 - EX28DRAFT_3468 0.17 +0.9 139,724 - EX28DRAFT_3468 0.17 +1.1 139,759 + EX28DRAFT_3468 0.28 +0.7 139,760 - EX28DRAFT_3468 0.28 +0.8 139,760 - EX28DRAFT_3468 0.28 +0.4 139,847 - EX28DRAFT_3468 0.55 -0.2 139,960 + -1.6 139,960 + +2.5 139,961 - -0.5 139,961 - -1.1 139,977 - -0.7 139,985 - -0.1 139,990 + -1.2 139,991 - -0.4 139,991 - -0.0 140,020 + -0.8 140,038 - +0.1 140,059 + +0.2 140,095 + -0.4 140,095 + -0.2 140,129 - -0.0 140,129 - +0.4 140,149 - +0.3 140,153 + +0.5 140,172 + -2.1 140,172 + +0.1 140,172 + +0.2 140,172 + -1.6 140,172 + +0.4 140,173 - +0.5 140,173 - +1.7 140,173 - -2.4 140,173 - +0.4 140,173 - +0.1 140,173 - +1.2 140,176 + -0.8 140,176 + -2.7 140,176 + -0.1 140,200 + +0.5 140,219 + -0.6 140,219 + -2.5 140,220 - -0.5 140,220 - +0.6 140,267 + EX28DRAFT_3469 0.19 +0.4 140,308 + EX28DRAFT_3469 0.29 -0.3 140,312 + EX28DRAFT_3469 0.30 +1.5 140,313 - EX28DRAFT_3469 0.31 +0.0 140,317 + EX28DRAFT_3469 0.32 -0.0 140,317 + EX28DRAFT_3469 0.32 +0.6 140,318 - EX28DRAFT_3469 0.32 +0.0 140,433 - EX28DRAFT_3469 0.60 -0.0 140,433 - EX28DRAFT_3469 0.60 +2.0 140,469 - EX28DRAFT_3469 0.69 +0.7 140,548 - EX28DRAFT_3469 0.88 -1.8 140,567 + +0.3 140,568 - +1.3 140,631 + +0.3 140,632 - -0.2 140,632 - -1.7 140,634 + +0.1 140,635 - +1.2 140,638 + EX28DRAFT_3470 0.10 +0.5 140,639 - EX28DRAFT_3470 0.11 +0.8 140,640 + EX28DRAFT_3470 0.11 -0.9 140,640 + EX28DRAFT_3470 0.11 +2.2 140,672 - EX28DRAFT_3470 0.22 -0.1 140,708 + EX28DRAFT_3470 0.34 +0.5 140,726 + EX28DRAFT_3470 0.41 -0.7 140,726 + EX28DRAFT_3470 0.41 -0.5 140,727 - EX28DRAFT_3470 0.41 -2.5 140,727 - EX28DRAFT_3470 0.41 -0.5 140,727 - EX28DRAFT_3470 0.41 +0.2 140,727 - EX28DRAFT_3470 0.41 +0.9 140,727 - EX28DRAFT_3470 0.41 +0.2 140,727 - EX28DRAFT_3470 0.41 -0.4 140,727 - EX28DRAFT_3470 0.41 +0.4 140,745 + EX28DRAFT_3470 0.47 +0.5 140,746 - EX28DRAFT_3470 0.47 -0.5 140,763 + EX28DRAFT_3470 0.53 -0.0 140,779 - EX28DRAFT_3470 0.59 +1.6 140,789 + EX28DRAFT_3470 0.62 +0.8 140,790 - EX28DRAFT_3470 0.63 +0.4 140,790 - EX28DRAFT_3470 0.63 -0.3 140,800 - EX28DRAFT_3470 0.66 -0.5 140,802 + EX28DRAFT_3470 0.67 -0.0 140,802 + EX28DRAFT_3470 0.67 +0.4 140,803 - EX28DRAFT_3470 0.67 -0.5 140,831 + EX28DRAFT_3470 0.77 -2.2 140,831 + EX28DRAFT_3470 0.77 -0.2 140,831 + EX28DRAFT_3470 0.77 +0.6 140,831 + EX28DRAFT_3470 0.77 -0.1 140,832 - EX28DRAFT_3470 0.77 +0.6 140,832 - EX28DRAFT_3470 0.77 -0.3 140,836 + EX28DRAFT_3470 0.78 +0.7 140,885 + +0.2 140,903 - -0.5 140,954 + EX28DRAFT_3471 0.15 -0.0 140,986 + EX28DRAFT_3471 0.31 +1.3 140,987 - EX28DRAFT_3471 0.31 -1.5 140,987 - EX28DRAFT_3471 0.31 -0.0
Or see this region's nucleotide sequence