Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3128 and EX28DRAFT_3129 are separated by 1 nucleotides EX28DRAFT_3129 and EX28DRAFT_3130 overlap by 1 nucleotides EX28DRAFT_3130 and EX28DRAFT_3131 are separated by 68 nucleotides EX28DRAFT_3131 and EX28DRAFT_3132 are separated by 227 nucleotides
EX28DRAFT_3128: EX28DRAFT_3128 - Bacteriophage replication gene A protein (GPA), at 429,552 to 431,741
_3128
EX28DRAFT_3129: EX28DRAFT_3129 - transcriptional regulator, TraR/DksA family, at 431,743 to 431,964
_3129
EX28DRAFT_3130: EX28DRAFT_3130 - Protein of unknown function (DUF2732), at 431,964 to 432,191
_3130
EX28DRAFT_3131: EX28DRAFT_3131 - hypothetical protein, at 432,260 to 432,598
_3131
EX28DRAFT_3132: EX28DRAFT_3132 - Bacteriophage CI repressor helix-turn-helix domain, at 432,826 to 433,401
_3132
Position (kb) 431
432
433 Strain fitness (log2 ratio) -2
-1
0
1 at 431.224 kb on + strand, within EX28DRAFT_3128 at 431.225 kb on - strand, within EX28DRAFT_3128 at 431.233 kb on + strand, within EX28DRAFT_3128 at 431.246 kb on - strand, within EX28DRAFT_3128 at 431.268 kb on - strand, within EX28DRAFT_3128 at 431.356 kb on + strand, within EX28DRAFT_3128 at 431.392 kb on + strand, within EX28DRAFT_3128 at 431.392 kb on + strand, within EX28DRAFT_3128 at 431.393 kb on - strand, within EX28DRAFT_3128 at 431.393 kb on - strand, within EX28DRAFT_3128 at 431.467 kb on + strand, within EX28DRAFT_3128 at 431.468 kb on - strand, within EX28DRAFT_3128 at 431.468 kb on - strand, within EX28DRAFT_3128 at 431.683 kb on + strand at 431.684 kb on - strand at 431.736 kb on - strand at 431.744 kb on + strand at 431.745 kb on - strand at 431.745 kb on - strand at 431.763 kb on + strand at 431.771 kb on - strand, within EX28DRAFT_3129 at 431.909 kb on + strand, within EX28DRAFT_3129 at 431.909 kb on + strand, within EX28DRAFT_3129 at 431.909 kb on + strand, within EX28DRAFT_3129 at 432.053 kb on - strand, within EX28DRAFT_3130 at 432.053 kb on - strand, within EX28DRAFT_3130 at 432.053 kb on - strand, within EX28DRAFT_3130 at 432.057 kb on - strand, within EX28DRAFT_3130 at 432.205 kb on + strand at 432.244 kb on + strand at 432.261 kb on + strand at 432.261 kb on + strand at 432.261 kb on + strand at 432.599 kb on - strand at 432.599 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 431,224 + EX28DRAFT_3128 0.76 -0.1 431,225 - EX28DRAFT_3128 0.76 -0.8 431,233 + EX28DRAFT_3128 0.77 +0.3 431,246 - EX28DRAFT_3128 0.77 -2.3 431,268 - EX28DRAFT_3128 0.78 +0.7 431,356 + EX28DRAFT_3128 0.82 -0.4 431,392 + EX28DRAFT_3128 0.84 -0.2 431,392 + EX28DRAFT_3128 0.84 -0.2 431,393 - EX28DRAFT_3128 0.84 -0.3 431,393 - EX28DRAFT_3128 0.84 -2.1 431,467 + EX28DRAFT_3128 0.87 -2.3 431,468 - EX28DRAFT_3128 0.87 -2.1 431,468 - EX28DRAFT_3128 0.87 -2.3 431,683 + -0.7 431,684 - +0.5 431,736 - -0.4 431,744 + +0.0 431,745 - +0.7 431,745 - -0.1 431,763 + +0.4 431,771 - EX28DRAFT_3129 0.13 -0.7 431,909 + EX28DRAFT_3129 0.75 -0.3 431,909 + EX28DRAFT_3129 0.75 +1.3 431,909 + EX28DRAFT_3129 0.75 -0.6 432,053 - EX28DRAFT_3130 0.39 +1.2 432,053 - EX28DRAFT_3130 0.39 -0.5 432,053 - EX28DRAFT_3130 0.39 +0.3 432,057 - EX28DRAFT_3130 0.41 +0.8 432,205 + +0.9 432,244 + -0.7 432,261 + +0.6 432,261 + +0.5 432,261 + +0.7 432,599 - -0.0 432,599 - +0.2
Or see this region's nucleotide sequence