Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2084 and EX28DRAFT_2085 overlap by 4 nucleotides EX28DRAFT_2085 and EX28DRAFT_2086 are separated by 97 nucleotides EX28DRAFT_2086 and EX28DRAFT_2087 are separated by 226 nucleotides EX28DRAFT_2087 and EX28DRAFT_2088 are separated by 19 nucleotides
EX28DRAFT_2084: EX28DRAFT_2084 - Negative regulator of beta-lactamase expression, at 2,110,023 to 2,110,853
_2084
EX28DRAFT_2085: EX28DRAFT_2085 - Uncharacterized conserved protein, at 2,110,850 to 2,111,173
_2085
EX28DRAFT_2086: EX28DRAFT_2086 - hypothetical protein, at 2,111,271 to 2,111,789
_2086
EX28DRAFT_2087: EX28DRAFT_2087 - ABC-type arginine transport system, ATPase component, at 2,112,016 to 2,112,744
_2087
EX28DRAFT_2088: EX28DRAFT_2088 - lysine-arginine-ornithine-binding periplasmic protein, at 2,112,764 to 2,113,495
_2088
Position (kb)
2111
2112 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2110.334 kb on + strand, within EX28DRAFT_2084 at 2110.397 kb on + strand, within EX28DRAFT_2084 at 2110.639 kb on + strand, within EX28DRAFT_2084 at 2110.702 kb on + strand, within EX28DRAFT_2084 at 2110.722 kb on + strand, within EX28DRAFT_2084 at 2110.722 kb on + strand, within EX28DRAFT_2084 at 2110.723 kb on - strand, within EX28DRAFT_2084 at 2111.125 kb on - strand, within EX28DRAFT_2085 at 2111.133 kb on - strand, within EX28DRAFT_2085 at 2111.234 kb on - strand at 2111.251 kb on + strand at 2111.252 kb on - strand at 2111.258 kb on + strand at 2111.259 kb on - strand at 2111.259 kb on - strand at 2111.418 kb on + strand, within EX28DRAFT_2086 at 2111.418 kb on + strand, within EX28DRAFT_2086 at 2111.530 kb on + strand, within EX28DRAFT_2086 at 2111.531 kb on - strand, within EX28DRAFT_2086 at 2111.915 kb on - strand at 2111.936 kb on + strand at 2111.965 kb on - strand at 2111.985 kb on + strand at 2111.986 kb on - strand at 2111.986 kb on - strand at 2111.986 kb on - strand at 2111.986 kb on - strand at 2112.002 kb on + strand at 2112.003 kb on - strand at 2112.025 kb on - strand at 2112.029 kb on + strand at 2112.040 kb on - strand at 2112.040 kb on - strand at 2112.049 kb on + strand at 2112.287 kb on - strand, within EX28DRAFT_2087 at 2112.287 kb on - strand, within EX28DRAFT_2087 at 2112.416 kb on - strand, within EX28DRAFT_2087 at 2112.588 kb on + strand, within EX28DRAFT_2087 at 2112.588 kb on + strand, within EX28DRAFT_2087 at 2112.589 kb on - strand, within EX28DRAFT_2087 at 2112.589 kb on - strand, within EX28DRAFT_2087 at 2112.592 kb on - strand, within EX28DRAFT_2087 at 2112.652 kb on + strand, within EX28DRAFT_2087 at 2112.652 kb on + strand, within EX28DRAFT_2087 at 2112.652 kb on + strand, within EX28DRAFT_2087 at 2112.653 kb on - strand, within EX28DRAFT_2087 at 2112.653 kb on - strand, within EX28DRAFT_2087 at 2112.653 kb on - strand, within EX28DRAFT_2087 at 2112.653 kb on - strand, within EX28DRAFT_2087 at 2112.670 kb on - strand, within EX28DRAFT_2087 at 2112.731 kb on - strand at 2112.734 kb on + strand at 2112.735 kb on - strand at 2112.760 kb on + strand at 2112.761 kb on - strand at 2112.761 kb on - strand at 2112.778 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,110,334 + EX28DRAFT_2084 0.37 -0.6 2,110,397 + EX28DRAFT_2084 0.45 +1.1 2,110,639 + EX28DRAFT_2084 0.74 +1.7 2,110,702 + EX28DRAFT_2084 0.82 -1.8 2,110,722 + EX28DRAFT_2084 0.84 -0.8 2,110,722 + EX28DRAFT_2084 0.84 -0.7 2,110,723 - EX28DRAFT_2084 0.84 -0.7 2,111,125 - EX28DRAFT_2085 0.85 -0.0 2,111,133 - EX28DRAFT_2085 0.87 +0.9 2,111,234 - +2.2 2,111,251 + -0.2 2,111,252 - -1.1 2,111,258 + +0.7 2,111,259 - +0.0 2,111,259 - +0.3 2,111,418 + EX28DRAFT_2086 0.28 -0.0 2,111,418 + EX28DRAFT_2086 0.28 -0.6 2,111,530 + EX28DRAFT_2086 0.50 +2.3 2,111,531 - EX28DRAFT_2086 0.50 +0.5 2,111,915 - -0.3 2,111,936 + +1.1 2,111,965 - -0.5 2,111,985 + +0.6 2,111,986 - -0.9 2,111,986 - +0.4 2,111,986 - -0.3 2,111,986 - -0.6 2,112,002 + +0.3 2,112,003 - -0.0 2,112,025 - -1.3 2,112,029 + +0.1 2,112,040 - +0.6 2,112,040 - +0.2 2,112,049 + +0.9 2,112,287 - EX28DRAFT_2087 0.37 +1.5 2,112,287 - EX28DRAFT_2087 0.37 -0.1 2,112,416 - EX28DRAFT_2087 0.55 -0.5 2,112,588 + EX28DRAFT_2087 0.78 +0.7 2,112,588 + EX28DRAFT_2087 0.78 -1.9 2,112,589 - EX28DRAFT_2087 0.79 +0.3 2,112,589 - EX28DRAFT_2087 0.79 +1.6 2,112,592 - EX28DRAFT_2087 0.79 -0.3 2,112,652 + EX28DRAFT_2087 0.87 -0.5 2,112,652 + EX28DRAFT_2087 0.87 +0.6 2,112,652 + EX28DRAFT_2087 0.87 +0.8 2,112,653 - EX28DRAFT_2087 0.87 -0.1 2,112,653 - EX28DRAFT_2087 0.87 -0.3 2,112,653 - EX28DRAFT_2087 0.87 -0.3 2,112,653 - EX28DRAFT_2087 0.87 +1.0 2,112,670 - EX28DRAFT_2087 0.90 +0.5 2,112,731 - +0.0 2,112,734 + -2.0 2,112,735 - +0.3 2,112,760 + -0.5 2,112,761 - -0.1 2,112,761 - -3.2 2,112,778 - -1.4
Or see this region's nucleotide sequence