Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1745 and EX28DRAFT_1746 overlap by 23 nucleotides EX28DRAFT_1746 and EX28DRAFT_1747 are separated by 297 nucleotides EX28DRAFT_1747 and EX28DRAFT_1748 are separated by 338 nucleotides
EX28DRAFT_1745: EX28DRAFT_1745 - GIY-YIG catalytic domain, at 1,759,700 to 1,760,605
_1745
EX28DRAFT_1746: EX28DRAFT_1746 - Uncharacterized protein conserved in bacteria, at 1,760,583 to 1,761,140
_1746
EX28DRAFT_1747: EX28DRAFT_1747 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 1,761,438 to 1,761,932
_1747
EX28DRAFT_1748: EX28DRAFT_1748 - succinylglutamate desuccinylase (EC 3.5.1.96), at 1,762,271 to 1,763,236
_1748
Position (kb)
1761
1762 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1760.533 kb on + strand at 1760.545 kb on + strand at 1760.577 kb on + strand at 1760.578 kb on - strand at 1760.578 kb on - strand at 1760.619 kb on + strand at 1760.619 kb on + strand at 1760.620 kb on - strand at 1760.651 kb on + strand, within EX28DRAFT_1746 at 1760.822 kb on + strand, within EX28DRAFT_1746 at 1761.120 kb on - strand at 1761.461 kb on + strand at 1761.858 kb on - strand, within EX28DRAFT_1747 at 1761.927 kb on - strand at 1762.002 kb on + strand at 1762.147 kb on - strand at 1762.215 kb on + strand at 1762.216 kb on - strand at 1762.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,760,533 + +0.3 1,760,545 + +2.2 1,760,577 + -0.8 1,760,578 - +1.9 1,760,578 - -1.6 1,760,619 + +0.8 1,760,619 + -0.0 1,760,620 - -4.0 1,760,651 + EX28DRAFT_1746 0.12 -0.5 1,760,822 + EX28DRAFT_1746 0.43 -0.1 1,761,120 - -0.3 1,761,461 + +0.2 1,761,858 - EX28DRAFT_1747 0.85 +0.5 1,761,927 - -2.5 1,762,002 + -0.7 1,762,147 - -1.1 1,762,215 + +0.6 1,762,216 - +0.2 1,762,342 - -0.7
Or see this region's nucleotide sequence