Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_1747

Experiment: M9 with sucrose and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_1745 and EX28DRAFT_1746 overlap by 23 nucleotidesEX28DRAFT_1746 and EX28DRAFT_1747 are separated by 297 nucleotidesEX28DRAFT_1747 and EX28DRAFT_1748 are separated by 338 nucleotides EX28DRAFT_1745: EX28DRAFT_1745 - GIY-YIG catalytic domain, at 1,759,700 to 1,760,605 _1745 EX28DRAFT_1746: EX28DRAFT_1746 - Uncharacterized protein conserved in bacteria, at 1,760,583 to 1,761,140 _1746 EX28DRAFT_1747: EX28DRAFT_1747 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 1,761,438 to 1,761,932 _1747 EX28DRAFT_1748: EX28DRAFT_1748 - succinylglutamate desuccinylase (EC 3.5.1.96), at 1,762,271 to 1,763,236 _1748 Position (kb) 1761 1762Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1760.533 kb on + strandat 1760.545 kb on + strandat 1760.577 kb on + strandat 1760.578 kb on - strandat 1760.578 kb on - strandat 1760.619 kb on + strandat 1760.619 kb on + strandat 1760.620 kb on - strandat 1760.651 kb on + strand, within EX28DRAFT_1746at 1760.822 kb on + strand, within EX28DRAFT_1746at 1761.120 kb on - strandat 1761.461 kb on + strandat 1761.858 kb on - strand, within EX28DRAFT_1747at 1761.927 kb on - strandat 1762.002 kb on + strandat 1762.147 kb on - strandat 1762.215 kb on + strandat 1762.216 kb on - strandat 1762.342 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM
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1,760,533 + +0.3
1,760,545 + +2.2
1,760,577 + -0.8
1,760,578 - +1.9
1,760,578 - -1.6
1,760,619 + +0.8
1,760,619 + -0.0
1,760,620 - -4.0
1,760,651 + EX28DRAFT_1746 0.12 -0.5
1,760,822 + EX28DRAFT_1746 0.43 -0.1
1,761,120 - -0.3
1,761,461 + +0.2
1,761,858 - EX28DRAFT_1747 0.85 +0.5
1,761,927 - -2.5
1,762,002 + -0.7
1,762,147 - -1.1
1,762,215 + +0.6
1,762,216 - +0.2
1,762,342 - -0.7

Or see this region's nucleotide sequence