Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1225 and EX28DRAFT_1226 are separated by 174 nucleotides EX28DRAFT_1226 and EX28DRAFT_1227 are separated by 34 nucleotides EX28DRAFT_1227 and EX28DRAFT_1228 are separated by 135 nucleotides
EX28DRAFT_1225: EX28DRAFT_1225 - Helix-turn-helix domain, at 1,243,084 to 1,243,956
_1225
EX28DRAFT_1226: EX28DRAFT_1226 - EamA-like transporter family/Multidrug resistance efflux transporter, at 1,244,131 to 1,245,027
_1226
EX28DRAFT_1227: EX28DRAFT_1227 - Protein of unknown function (DUF3313), at 1,245,062 to 1,245,736
_1227
EX28DRAFT_1228: EX28DRAFT_1228 - ABC-type dipeptide transport system, periplasmic component, at 1,245,872 to 1,247,467
_1228
Position (kb)
1244
1245
1246 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1243.253 kb on + strand, within EX28DRAFT_1225 at 1243.253 kb on + strand, within EX28DRAFT_1225 at 1243.253 kb on + strand, within EX28DRAFT_1225 at 1243.254 kb on - strand, within EX28DRAFT_1225 at 1243.449 kb on - strand, within EX28DRAFT_1225 at 1243.661 kb on + strand, within EX28DRAFT_1225 at 1243.803 kb on - strand, within EX28DRAFT_1225 at 1243.816 kb on - strand, within EX28DRAFT_1225 at 1243.856 kb on + strand, within EX28DRAFT_1225 at 1243.939 kb on - strand at 1243.992 kb on - strand at 1244.131 kb on - strand at 1244.141 kb on + strand at 1244.142 kb on - strand at 1244.325 kb on + strand, within EX28DRAFT_1226 at 1244.356 kb on - strand, within EX28DRAFT_1226 at 1244.434 kb on + strand, within EX28DRAFT_1226 at 1244.434 kb on + strand, within EX28DRAFT_1226 at 1244.434 kb on + strand, within EX28DRAFT_1226 at 1244.606 kb on - strand, within EX28DRAFT_1226 at 1244.764 kb on + strand, within EX28DRAFT_1226 at 1244.764 kb on + strand, within EX28DRAFT_1226 at 1244.783 kb on - strand, within EX28DRAFT_1226 at 1244.822 kb on - strand, within EX28DRAFT_1226 at 1244.842 kb on - strand, within EX28DRAFT_1226 at 1244.842 kb on - strand, within EX28DRAFT_1226 at 1244.864 kb on - strand, within EX28DRAFT_1226 at 1244.905 kb on - strand, within EX28DRAFT_1226 at 1244.912 kb on - strand, within EX28DRAFT_1226 at 1244.912 kb on - strand, within EX28DRAFT_1226 at 1244.920 kb on + strand, within EX28DRAFT_1226 at 1244.921 kb on - strand, within EX28DRAFT_1226 at 1244.921 kb on - strand, within EX28DRAFT_1226 at 1244.940 kb on + strand at 1244.941 kb on - strand at 1244.973 kb on + strand at 1244.974 kb on - strand at 1244.974 kb on - strand at 1244.986 kb on - strand at 1245.025 kb on + strand at 1245.059 kb on - strand at 1245.059 kb on - strand at 1245.120 kb on - strand at 1245.286 kb on + strand, within EX28DRAFT_1227 at 1245.321 kb on + strand, within EX28DRAFT_1227 at 1245.322 kb on - strand, within EX28DRAFT_1227 at 1245.322 kb on - strand, within EX28DRAFT_1227 at 1245.511 kb on - strand, within EX28DRAFT_1227 at 1245.517 kb on + strand, within EX28DRAFT_1227 at 1245.518 kb on - strand, within EX28DRAFT_1227 at 1245.535 kb on + strand, within EX28DRAFT_1227 at 1245.558 kb on + strand, within EX28DRAFT_1227 at 1245.559 kb on - strand, within EX28DRAFT_1227 at 1245.559 kb on - strand, within EX28DRAFT_1227 at 1245.625 kb on - strand, within EX28DRAFT_1227 at 1245.632 kb on + strand, within EX28DRAFT_1227 at 1245.645 kb on + strand, within EX28DRAFT_1227 at 1245.646 kb on - strand, within EX28DRAFT_1227 at 1245.701 kb on - strand at 1245.752 kb on + strand at 1245.753 kb on - strand at 1245.763 kb on - strand at 1245.763 kb on - strand at 1246.023 kb on + strand at 1246.024 kb on - strand at 1246.024 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,243,253 + EX28DRAFT_1225 0.19 -0.5 1,243,253 + EX28DRAFT_1225 0.19 -0.5 1,243,253 + EX28DRAFT_1225 0.19 +0.9 1,243,254 - EX28DRAFT_1225 0.19 +0.2 1,243,449 - EX28DRAFT_1225 0.42 -0.1 1,243,661 + EX28DRAFT_1225 0.66 +0.1 1,243,803 - EX28DRAFT_1225 0.82 +1.2 1,243,816 - EX28DRAFT_1225 0.84 +0.3 1,243,856 + EX28DRAFT_1225 0.88 +0.8 1,243,939 - -0.6 1,243,992 - +1.1 1,244,131 - -3.2 1,244,141 + +1.5 1,244,142 - +0.5 1,244,325 + EX28DRAFT_1226 0.22 +0.2 1,244,356 - EX28DRAFT_1226 0.25 +0.3 1,244,434 + EX28DRAFT_1226 0.34 +0.5 1,244,434 + EX28DRAFT_1226 0.34 -0.8 1,244,434 + EX28DRAFT_1226 0.34 +1.6 1,244,606 - EX28DRAFT_1226 0.53 +1.3 1,244,764 + EX28DRAFT_1226 0.71 +1.8 1,244,764 + EX28DRAFT_1226 0.71 +1.3 1,244,783 - EX28DRAFT_1226 0.73 +0.1 1,244,822 - EX28DRAFT_1226 0.77 +0.5 1,244,842 - EX28DRAFT_1226 0.79 -3.2 1,244,842 - EX28DRAFT_1226 0.79 +0.7 1,244,864 - EX28DRAFT_1226 0.82 +0.6 1,244,905 - EX28DRAFT_1226 0.86 +1.5 1,244,912 - EX28DRAFT_1226 0.87 -1.4 1,244,912 - EX28DRAFT_1226 0.87 +0.6 1,244,920 + EX28DRAFT_1226 0.88 +2.0 1,244,921 - EX28DRAFT_1226 0.88 +0.5 1,244,921 - EX28DRAFT_1226 0.88 +1.4 1,244,940 + +0.9 1,244,941 - +1.2 1,244,973 + -0.0 1,244,974 - -0.4 1,244,974 - +0.2 1,244,986 - -0.8 1,245,025 + +1.4 1,245,059 - +0.1 1,245,059 - -0.5 1,245,120 - +0.5 1,245,286 + EX28DRAFT_1227 0.33 -2.4 1,245,321 + EX28DRAFT_1227 0.38 -3.2 1,245,322 - EX28DRAFT_1227 0.39 +0.3 1,245,322 - EX28DRAFT_1227 0.39 -1.0 1,245,511 - EX28DRAFT_1227 0.67 -0.1 1,245,517 + EX28DRAFT_1227 0.67 -0.3 1,245,518 - EX28DRAFT_1227 0.68 +0.2 1,245,535 + EX28DRAFT_1227 0.70 -0.5 1,245,558 + EX28DRAFT_1227 0.73 -0.1 1,245,559 - EX28DRAFT_1227 0.74 -0.2 1,245,559 - EX28DRAFT_1227 0.74 -0.4 1,245,625 - EX28DRAFT_1227 0.83 +0.9 1,245,632 + EX28DRAFT_1227 0.84 -2.0 1,245,645 + EX28DRAFT_1227 0.86 -0.2 1,245,646 - EX28DRAFT_1227 0.87 -0.3 1,245,701 - -0.5 1,245,752 + +0.6 1,245,753 - +2.2 1,245,763 - +1.0 1,245,763 - +0.4 1,246,023 + -0.3 1,246,024 - +0.6 1,246,024 - -1.8
Or see this region's nucleotide sequence