Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0654 and EX28DRAFT_0655 are separated by 176 nucleotides EX28DRAFT_0655 and EX28DRAFT_0656 overlap by 26 nucleotides
EX28DRAFT_0654: EX28DRAFT_0654 - Ribose/xylose/arabinose/galactoside ABC-type transport systems, permease components, at 648,548 to 649,534
_0654
EX28DRAFT_0655: EX28DRAFT_0655 - trehalose-phosphatase, at 649,711 to 650,514
_0655
EX28DRAFT_0656: EX28DRAFT_0656 - alpha,alpha-trehalose-phosphate synthase [UDP-forming], at 650,489 to 651,913
_0656
Position (kb) 649
650
651 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 648.986 kb on + strand, within EX28DRAFT_0654 at 648.988 kb on + strand, within EX28DRAFT_0654 at 648.988 kb on + strand, within EX28DRAFT_0654 at 648.989 kb on - strand, within EX28DRAFT_0654 at 648.989 kb on - strand, within EX28DRAFT_0654 at 649.145 kb on - strand, within EX28DRAFT_0654 at 649.213 kb on + strand, within EX28DRAFT_0654 at 649.258 kb on + strand, within EX28DRAFT_0654 at 649.259 kb on - strand, within EX28DRAFT_0654 at 649.259 kb on - strand, within EX28DRAFT_0654 at 649.379 kb on + strand, within EX28DRAFT_0654 at 649.380 kb on - strand, within EX28DRAFT_0654 at 649.380 kb on - strand, within EX28DRAFT_0654 at 649.457 kb on + strand at 649.565 kb on - strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.572 kb on + strand at 649.647 kb on - strand at 649.649 kb on - strand at 649.649 kb on - strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.652 kb on + strand at 649.753 kb on + strand at 649.754 kb on - strand at 649.754 kb on - strand at 649.819 kb on - strand, within EX28DRAFT_0655 at 649.992 kb on + strand, within EX28DRAFT_0655 at 650.213 kb on + strand, within EX28DRAFT_0655 at 650.214 kb on - strand, within EX28DRAFT_0655 at 650.232 kb on + strand, within EX28DRAFT_0655 at 650.430 kb on - strand, within EX28DRAFT_0655 at 650.462 kb on + strand at 650.463 kb on - strand at 650.499 kb on + strand at 650.574 kb on + strand at 650.574 kb on + strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.575 kb on - strand at 650.671 kb on + strand, within EX28DRAFT_0656 at 650.671 kb on + strand, within EX28DRAFT_0656 at 650.705 kb on + strand, within EX28DRAFT_0656 at 650.714 kb on + strand, within EX28DRAFT_0656 at 650.717 kb on + strand, within EX28DRAFT_0656 at 650.718 kb on - strand, within EX28DRAFT_0656 at 650.760 kb on - strand, within EX28DRAFT_0656 at 651.088 kb on + strand, within EX28DRAFT_0656 at 651.089 kb on - strand, within EX28DRAFT_0656 at 651.233 kb on - strand, within EX28DRAFT_0656
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 648,986 + EX28DRAFT_0654 0.44 +0.1 648,988 + EX28DRAFT_0654 0.45 +0.2 648,988 + EX28DRAFT_0654 0.45 -0.4 648,989 - EX28DRAFT_0654 0.45 -1.8 648,989 - EX28DRAFT_0654 0.45 -0.3 649,145 - EX28DRAFT_0654 0.60 -2.7 649,213 + EX28DRAFT_0654 0.67 -1.8 649,258 + EX28DRAFT_0654 0.72 +0.5 649,259 - EX28DRAFT_0654 0.72 -0.5 649,259 - EX28DRAFT_0654 0.72 -0.5 649,379 + EX28DRAFT_0654 0.84 +0.3 649,380 - EX28DRAFT_0654 0.84 +2.2 649,380 - EX28DRAFT_0654 0.84 +0.2 649,457 + +0.2 649,565 - +0.7 649,572 + -0.1 649,572 + -0.4 649,572 + +0.2 649,647 - -0.9 649,649 - +0.1 649,649 - -0.5 649,652 + -0.1 649,652 + -1.4 649,652 + +0.3 649,652 + -0.6 649,753 + +0.3 649,754 - +0.6 649,754 - +0.7 649,819 - EX28DRAFT_0655 0.13 +1.2 649,992 + EX28DRAFT_0655 0.35 +0.1 650,213 + EX28DRAFT_0655 0.62 -1.1 650,214 - EX28DRAFT_0655 0.63 +1.5 650,232 + EX28DRAFT_0655 0.65 +0.5 650,430 - EX28DRAFT_0655 0.89 +1.3 650,462 + +0.2 650,463 - -0.2 650,499 + +1.6 650,574 + -4.5 650,574 + -1.1 650,575 - -0.8 650,575 - -0.0 650,575 - +0.2 650,575 - -0.8 650,575 - +0.2 650,671 + EX28DRAFT_0656 0.13 -2.3 650,671 + EX28DRAFT_0656 0.13 +0.3 650,705 + EX28DRAFT_0656 0.15 +0.4 650,714 + EX28DRAFT_0656 0.16 -0.4 650,717 + EX28DRAFT_0656 0.16 +1.0 650,718 - EX28DRAFT_0656 0.16 -0.2 650,760 - EX28DRAFT_0656 0.19 +0.2 651,088 + EX28DRAFT_0656 0.42 -0.5 651,089 - EX28DRAFT_0656 0.42 -1.1 651,233 - EX28DRAFT_0656 0.52 +0.1
Or see this region's nucleotide sequence