Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0517 and EX28DRAFT_0518 are separated by 0 nucleotides EX28DRAFT_0518 and EX28DRAFT_0519 are separated by 38 nucleotides
EX28DRAFT_0517: EX28DRAFT_0517 - UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase, at 510,569 to 511,603
_0517
EX28DRAFT_0518: EX28DRAFT_0518 - Bacterial transferase hexapeptide (six repeats), at 511,604 to 512,164
_0518
EX28DRAFT_0519: EX28DRAFT_0519 - Predicted nucleoside-diphosphate sugar epimerases, at 512,203 to 514,089
_0519
Position (kb) 511
512
513 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 510.993 kb on - strand, within EX28DRAFT_0517 at 511.034 kb on - strand, within EX28DRAFT_0517 at 511.093 kb on - strand, within EX28DRAFT_0517 at 511.093 kb on - strand, within EX28DRAFT_0517 at 511.334 kb on - strand, within EX28DRAFT_0517 at 511.337 kb on - strand, within EX28DRAFT_0517 at 511.771 kb on + strand, within EX28DRAFT_0518 at 511.771 kb on + strand, within EX28DRAFT_0518 at 511.772 kb on - strand, within EX28DRAFT_0518 at 511.807 kb on + strand, within EX28DRAFT_0518 at 511.945 kb on - strand, within EX28DRAFT_0518 at 511.972 kb on + strand, within EX28DRAFT_0518 at 511.977 kb on + strand, within EX28DRAFT_0518 at 511.981 kb on + strand, within EX28DRAFT_0518 at 511.982 kb on - strand, within EX28DRAFT_0518 at 511.993 kb on + strand, within EX28DRAFT_0518 at 512.035 kb on - strand, within EX28DRAFT_0518 at 512.162 kb on + strand at 512.249 kb on + strand at 512.268 kb on - strand at 512.268 kb on - strand at 512.420 kb on + strand, within EX28DRAFT_0519 at 512.425 kb on + strand, within EX28DRAFT_0519 at 512.429 kb on + strand, within EX28DRAFT_0519 at 512.450 kb on + strand, within EX28DRAFT_0519 at 512.558 kb on + strand, within EX28DRAFT_0519 at 512.558 kb on + strand, within EX28DRAFT_0519 at 512.559 kb on - strand, within EX28DRAFT_0519 at 512.631 kb on - strand, within EX28DRAFT_0519 at 512.634 kb on + strand, within EX28DRAFT_0519 at 512.636 kb on + strand, within EX28DRAFT_0519 at 512.636 kb on + strand, within EX28DRAFT_0519 at 512.638 kb on + strand, within EX28DRAFT_0519 at 512.666 kb on + strand, within EX28DRAFT_0519 at 512.695 kb on + strand, within EX28DRAFT_0519 at 512.793 kb on - strand, within EX28DRAFT_0519 at 512.800 kb on + strand, within EX28DRAFT_0519 at 512.862 kb on + strand, within EX28DRAFT_0519 at 512.862 kb on + strand, within EX28DRAFT_0519 at 512.864 kb on + strand, within EX28DRAFT_0519 at 512.864 kb on + strand, within EX28DRAFT_0519 at 512.864 kb on + strand, within EX28DRAFT_0519 at 512.865 kb on - strand, within EX28DRAFT_0519 at 512.865 kb on - strand, within EX28DRAFT_0519 at 512.868 kb on + strand, within EX28DRAFT_0519 at 512.955 kb on + strand, within EX28DRAFT_0519 at 512.963 kb on + strand, within EX28DRAFT_0519 at 513.081 kb on - strand, within EX28DRAFT_0519
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 510,993 - EX28DRAFT_0517 0.41 +0.6 511,034 - EX28DRAFT_0517 0.45 -0.1 511,093 - EX28DRAFT_0517 0.51 +0.7 511,093 - EX28DRAFT_0517 0.51 +1.0 511,334 - EX28DRAFT_0517 0.74 +2.4 511,337 - EX28DRAFT_0517 0.74 -0.2 511,771 + EX28DRAFT_0518 0.30 +0.1 511,771 + EX28DRAFT_0518 0.30 +0.6 511,772 - EX28DRAFT_0518 0.30 +1.0 511,807 + EX28DRAFT_0518 0.36 +0.8 511,945 - EX28DRAFT_0518 0.61 +1.0 511,972 + EX28DRAFT_0518 0.66 -2.3 511,977 + EX28DRAFT_0518 0.66 -0.4 511,981 + EX28DRAFT_0518 0.67 +0.7 511,982 - EX28DRAFT_0518 0.67 +0.9 511,993 + EX28DRAFT_0518 0.69 +0.1 512,035 - EX28DRAFT_0518 0.77 +0.0 512,162 + +0.8 512,249 + +0.0 512,268 - +1.0 512,268 - -0.2 512,420 + EX28DRAFT_0519 0.11 +0.8 512,425 + EX28DRAFT_0519 0.12 +1.3 512,429 + EX28DRAFT_0519 0.12 +1.2 512,450 + EX28DRAFT_0519 0.13 +0.8 512,558 + EX28DRAFT_0519 0.19 +0.5 512,558 + EX28DRAFT_0519 0.19 +0.2 512,559 - EX28DRAFT_0519 0.19 -3.4 512,631 - EX28DRAFT_0519 0.23 +1.0 512,634 + EX28DRAFT_0519 0.23 +0.9 512,636 + EX28DRAFT_0519 0.23 -0.1 512,636 + EX28DRAFT_0519 0.23 +0.3 512,638 + EX28DRAFT_0519 0.23 +1.1 512,666 + EX28DRAFT_0519 0.25 +0.9 512,695 + EX28DRAFT_0519 0.26 +0.5 512,793 - EX28DRAFT_0519 0.31 +1.1 512,800 + EX28DRAFT_0519 0.32 +0.3 512,862 + EX28DRAFT_0519 0.35 -0.2 512,862 + EX28DRAFT_0519 0.35 -1.0 512,864 + EX28DRAFT_0519 0.35 -0.4 512,864 + EX28DRAFT_0519 0.35 +0.9 512,864 + EX28DRAFT_0519 0.35 +0.1 512,865 - EX28DRAFT_0519 0.35 +0.5 512,865 - EX28DRAFT_0519 0.35 +2.4 512,868 + EX28DRAFT_0519 0.35 +0.3 512,955 + EX28DRAFT_0519 0.40 -1.3 512,963 + EX28DRAFT_0519 0.40 +1.0 513,081 - EX28DRAFT_0519 0.47 -0.0
Or see this region's nucleotide sequence