Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3638 and EX28DRAFT_3639 are separated by 138 nucleotides EX28DRAFT_3639 and EX28DRAFT_3640 are separated by 103 nucleotides EX28DRAFT_3640 and EX28DRAFT_3641 overlap by 8 nucleotides EX28DRAFT_3641 and EX28DRAFT_3642 are separated by 114 nucleotides EX28DRAFT_3642 and EX28DRAFT_3643 are separated by 39 nucleotides
EX28DRAFT_3638: EX28DRAFT_3638 - carbamoyl-phosphate synthase, large subunit, at 298,604 to 301,828
_3638
EX28DRAFT_3639: EX28DRAFT_3639 - Bacterial protein of unknown function (DUF903), at 301,967 to 302,200
_3639
EX28DRAFT_3640: EX28DRAFT_3640 - Putative NADPH-quinone reductase (modulator of drug activity B), at 302,304 to 302,834
_3640
EX28DRAFT_3641: EX28DRAFT_3641 - transporter, monovalent cation:proton antiporter-2 (CPA2) family, at 302,827 to 304,692
_3641
EX28DRAFT_3642: EX28DRAFT_3642 - Dihydrofolate reductase, at 304,807 to 305,286
_3642
EX28DRAFT_3643: EX28DRAFT_3643 - Bis(5'nucleosyl)-tetraphosphatase, ApaH, at 305,326 to 306,174
_3643
Position (kb) 302
303
304
305 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 302.097 kb on + strand, within EX28DRAFT_3639 at 302.097 kb on + strand, within EX28DRAFT_3639 at 302.124 kb on - strand, within EX28DRAFT_3639 at 302.126 kb on - strand, within EX28DRAFT_3639 at 302.194 kb on + strand at 302.198 kb on + strand at 302.198 kb on + strand at 302.198 kb on + strand at 302.199 kb on - strand at 302.199 kb on - strand at 302.216 kb on + strand at 302.216 kb on + strand at 302.231 kb on + strand at 302.232 kb on - strand at 302.232 kb on - strand at 302.238 kb on + strand at 302.239 kb on - strand at 302.239 kb on - strand at 302.254 kb on + strand at 302.254 kb on + strand at 302.262 kb on - strand at 302.262 kb on - strand at 302.287 kb on + strand at 302.319 kb on + strand at 302.375 kb on - strand, within EX28DRAFT_3640 at 302.375 kb on - strand, within EX28DRAFT_3640 at 302.392 kb on + strand, within EX28DRAFT_3640 at 302.392 kb on + strand, within EX28DRAFT_3640 at 302.398 kb on + strand, within EX28DRAFT_3640 at 302.398 kb on + strand, within EX28DRAFT_3640 at 302.398 kb on + strand, within EX28DRAFT_3640 at 302.399 kb on - strand, within EX28DRAFT_3640 at 302.399 kb on - strand, within EX28DRAFT_3640 at 302.399 kb on - strand, within EX28DRAFT_3640 at 302.410 kb on - strand, within EX28DRAFT_3640 at 302.419 kb on - strand, within EX28DRAFT_3640 at 302.429 kb on + strand, within EX28DRAFT_3640 at 302.433 kb on - strand, within EX28DRAFT_3640 at 302.481 kb on - strand, within EX28DRAFT_3640 at 302.505 kb on + strand, within EX28DRAFT_3640 at 302.505 kb on + strand, within EX28DRAFT_3640 at 302.540 kb on + strand, within EX28DRAFT_3640 at 302.566 kb on - strand, within EX28DRAFT_3640 at 302.566 kb on - strand, within EX28DRAFT_3640 at 302.791 kb on - strand at 302.792 kb on + strand at 302.792 kb on + strand at 302.793 kb on - strand at 302.793 kb on - strand at 302.838 kb on + strand at 302.860 kb on + strand at 303.178 kb on - strand, within EX28DRAFT_3641 at 303.352 kb on - strand, within EX28DRAFT_3641 at 303.428 kb on - strand, within EX28DRAFT_3641 at 303.744 kb on + strand, within EX28DRAFT_3641 at 303.759 kb on - strand, within EX28DRAFT_3641 at 304.439 kb on - strand, within EX28DRAFT_3641 at 304.711 kb on - strand at 304.711 kb on - strand at 304.769 kb on + strand at 305.319 kb on + strand at 305.324 kb on + strand at 305.325 kb on - strand at 305.408 kb on - strand at 305.600 kb on - strand, within EX28DRAFT_3643
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 302,097 + EX28DRAFT_3639 0.56 -0.8 302,097 + EX28DRAFT_3639 0.56 +0.6 302,124 - EX28DRAFT_3639 0.67 +1.5 302,126 - EX28DRAFT_3639 0.68 -1.1 302,194 + -1.4 302,198 + +0.0 302,198 + +0.3 302,198 + +0.5 302,199 - +0.2 302,199 - -0.2 302,216 + +0.1 302,216 + +0.2 302,231 + -0.1 302,232 - -0.2 302,232 - -1.3 302,238 + +0.4 302,239 - -1.6 302,239 - -0.5 302,254 + -0.3 302,254 + +0.3 302,262 - +0.2 302,262 - -1.3 302,287 + -0.0 302,319 + -0.1 302,375 - EX28DRAFT_3640 0.13 +0.8 302,375 - EX28DRAFT_3640 0.13 +0.1 302,392 + EX28DRAFT_3640 0.17 -0.3 302,392 + EX28DRAFT_3640 0.17 +0.1 302,398 + EX28DRAFT_3640 0.18 +0.3 302,398 + EX28DRAFT_3640 0.18 -4.6 302,398 + EX28DRAFT_3640 0.18 -0.1 302,399 - EX28DRAFT_3640 0.18 +0.5 302,399 - EX28DRAFT_3640 0.18 -1.7 302,399 - EX28DRAFT_3640 0.18 +0.5 302,410 - EX28DRAFT_3640 0.20 -0.8 302,419 - EX28DRAFT_3640 0.22 +0.7 302,429 + EX28DRAFT_3640 0.24 -0.6 302,433 - EX28DRAFT_3640 0.24 -0.1 302,481 - EX28DRAFT_3640 0.33 -0.0 302,505 + EX28DRAFT_3640 0.38 +0.7 302,505 + EX28DRAFT_3640 0.38 -0.2 302,540 + EX28DRAFT_3640 0.44 +0.9 302,566 - EX28DRAFT_3640 0.49 -1.5 302,566 - EX28DRAFT_3640 0.49 -0.0 302,791 - -0.0 302,792 + -0.3 302,792 + -0.4 302,793 - -0.5 302,793 - +1.7 302,838 + +0.4 302,860 + +0.3 303,178 - EX28DRAFT_3641 0.19 -0.6 303,352 - EX28DRAFT_3641 0.28 -0.5 303,428 - EX28DRAFT_3641 0.32 -0.5 303,744 + EX28DRAFT_3641 0.49 +0.1 303,759 - EX28DRAFT_3641 0.50 -0.1 304,439 - EX28DRAFT_3641 0.86 -0.6 304,711 - +0.7 304,711 - -1.2 304,769 + +0.2 305,319 + -0.2 305,324 + +0.2 305,325 - -0.4 305,408 - +0.2 305,600 - EX28DRAFT_3643 0.32 -0.1
Or see this region's nucleotide sequence