Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23600 and EX31_RS25660 are separated by 190 nucleotides EX31_RS25660 and EX31_RS23610 are separated by 195 nucleotides
EX31_RS23600: EX31_RS23600 - molybdenum-dependent transcriptional regulator, at 485,954 to 486,736
_RS23600
EX31_RS25660: EX31_RS25660 - multidrug efflux pump-associated protein, AcrZ family, at 486,927 to 487,082
_RS25660
EX31_RS23610: EX31_RS23610 - molybdate ABC transporter substrate-binding protein, at 487,278 to 488,051
_RS23610
Position (kb) 486
487
488 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 485.936 kb on - strand at 485.946 kb on + strand at 486.001 kb on - strand at 486.301 kb on + strand, within EX31_RS23600 at 486.301 kb on + strand, within EX31_RS23600 at 486.301 kb on + strand, within EX31_RS23600 at 486.301 kb on + strand, within EX31_RS23600 at 486.301 kb on + strand, within EX31_RS23600 at 486.301 kb on + strand, within EX31_RS23600 at 486.301 kb on + strand, within EX31_RS23600 at 486.302 kb on - strand, within EX31_RS23600 at 486.302 kb on - strand, within EX31_RS23600 at 486.302 kb on - strand, within EX31_RS23600 at 486.302 kb on - strand, within EX31_RS23600 at 486.302 kb on - strand, within EX31_RS23600 at 486.302 kb on - strand, within EX31_RS23600 at 486.335 kb on + strand, within EX31_RS23600 at 486.335 kb on + strand, within EX31_RS23600 at 486.336 kb on - strand, within EX31_RS23600 at 486.336 kb on - strand, within EX31_RS23600 at 486.336 kb on - strand, within EX31_RS23600 at 486.336 kb on - strand, within EX31_RS23600 at 486.428 kb on + strand, within EX31_RS23600 at 486.604 kb on - strand, within EX31_RS23600 at 486.665 kb on + strand at 486.666 kb on - strand at 486.666 kb on - strand at 486.674 kb on - strand at 486.716 kb on + strand at 486.717 kb on - strand at 486.752 kb on - strand at 486.764 kb on + strand at 486.764 kb on + strand at 486.764 kb on + strand at 486.765 kb on - strand at 486.801 kb on + strand at 486.802 kb on - strand at 486.802 kb on - strand at 486.808 kb on + strand at 486.813 kb on + strand at 486.813 kb on + strand at 486.813 kb on + strand at 486.813 kb on + strand at 486.813 kb on + strand at 486.813 kb on + strand at 486.814 kb on - strand at 486.814 kb on - strand at 486.814 kb on - strand at 486.817 kb on + strand at 486.833 kb on + strand at 486.834 kb on - strand at 486.836 kb on + strand at 486.875 kb on + strand at 486.875 kb on + strand at 486.875 kb on + strand at 486.875 kb on + strand at 486.875 kb on + strand at 486.875 kb on + strand at 486.913 kb on + strand at 486.913 kb on + strand at 486.913 kb on + strand at 486.913 kb on + strand at 486.913 kb on + strand at 486.914 kb on - strand at 486.914 kb on - strand at 486.923 kb on + strand at 486.924 kb on - strand at 486.931 kb on + strand at 486.931 kb on + strand at 486.931 kb on + strand at 486.931 kb on + strand at 486.932 kb on - strand at 486.932 kb on - strand at 486.937 kb on + strand at 486.937 kb on + strand at 486.941 kb on - strand at 487.021 kb on + strand, within EX31_RS25660 at 487.021 kb on + strand, within EX31_RS25660 at 487.052 kb on + strand, within EX31_RS25660 at 487.077 kb on + strand at 487.078 kb on - strand at 487.084 kb on + strand at 487.152 kb on - strand at 487.152 kb on - strand at 487.173 kb on + strand at 487.173 kb on + strand at 487.173 kb on + strand at 487.173 kb on + strand at 487.173 kb on + strand at 487.173 kb on + strand at 487.174 kb on - strand at 487.174 kb on - strand at 487.174 kb on - strand at 487.174 kb on - strand at 487.174 kb on - strand at 487.174 kb on - strand at 487.178 kb on + strand at 487.178 kb on + strand at 487.284 kb on + strand at 487.284 kb on + strand at 487.285 kb on - strand at 487.539 kb on + strand, within EX31_RS23610 at 487.539 kb on + strand, within EX31_RS23610 at 487.540 kb on - strand, within EX31_RS23610 at 487.569 kb on - strand, within EX31_RS23610 at 487.578 kb on + strand, within EX31_RS23610 at 487.778 kb on + strand, within EX31_RS23610 at 487.778 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.849 kb on + strand, within EX31_RS23610 at 487.850 kb on - strand, within EX31_RS23610 at 487.850 kb on - strand, within EX31_RS23610 at 487.850 kb on - strand, within EX31_RS23610 at 487.850 kb on - strand, within EX31_RS23610 at 487.850 kb on - strand, within EX31_RS23610 at 487.850 kb on - strand, within EX31_RS23610 at 487.855 kb on - strand, within EX31_RS23610 at 487.919 kb on + strand, within EX31_RS23610 at 487.919 kb on + strand, within EX31_RS23610 at 487.920 kb on - strand, within EX31_RS23610 at 487.983 kb on + strand at 487.983 kb on + strand at 487.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 485,936 - +0.1 485,946 + -2.8 486,001 - -0.7 486,301 + EX31_RS23600 0.44 -0.2 486,301 + EX31_RS23600 0.44 +0.6 486,301 + EX31_RS23600 0.44 +0.1 486,301 + EX31_RS23600 0.44 +0.4 486,301 + EX31_RS23600 0.44 -0.6 486,301 + EX31_RS23600 0.44 +1.0 486,301 + EX31_RS23600 0.44 +0.9 486,302 - EX31_RS23600 0.44 -0.8 486,302 - EX31_RS23600 0.44 -1.6 486,302 - EX31_RS23600 0.44 +1.1 486,302 - EX31_RS23600 0.44 +0.8 486,302 - EX31_RS23600 0.44 +0.1 486,302 - EX31_RS23600 0.44 -0.1 486,335 + EX31_RS23600 0.49 +0.1 486,335 + EX31_RS23600 0.49 -0.5 486,336 - EX31_RS23600 0.49 +1.3 486,336 - EX31_RS23600 0.49 +0.4 486,336 - EX31_RS23600 0.49 +1.5 486,336 - EX31_RS23600 0.49 +0.3 486,428 + EX31_RS23600 0.61 +0.7 486,604 - EX31_RS23600 0.83 +0.8 486,665 + -0.2 486,666 - -0.1 486,666 - +1.2 486,674 - +0.2 486,716 + -0.7 486,717 - -0.7 486,752 - +0.2 486,764 + -1.2 486,764 + +0.9 486,764 + +0.7 486,765 - -0.1 486,801 + +0.3 486,802 - +1.4 486,802 - -0.5 486,808 + -0.3 486,813 + +0.3 486,813 + +0.3 486,813 + +0.9 486,813 + +0.4 486,813 + +1.8 486,813 + -0.9 486,814 - +0.1 486,814 - +0.5 486,814 - +0.1 486,817 + -0.2 486,833 + -0.2 486,834 - +0.9 486,836 + +0.3 486,875 + -0.1 486,875 + -1.2 486,875 + -0.3 486,875 + -0.8 486,875 + +0.0 486,875 + -0.2 486,913 + -0.2 486,913 + +0.2 486,913 + -1.9 486,913 + -0.3 486,913 + -0.7 486,914 - +0.3 486,914 - +0.4 486,923 + -0.1 486,924 - -1.2 486,931 + -0.2 486,931 + -1.3 486,931 + -1.3 486,931 + +2.0 486,932 - +1.0 486,932 - +0.2 486,937 + -0.6 486,937 + +0.3 486,941 - +0.1 487,021 + EX31_RS25660 0.60 -2.0 487,021 + EX31_RS25660 0.60 -3.0 487,052 + EX31_RS25660 0.80 -0.5 487,077 + +0.6 487,078 - +0.3 487,084 + -0.1 487,152 - +0.4 487,152 - +0.5 487,173 + +0.9 487,173 + +0.3 487,173 + -0.2 487,173 + +0.7 487,173 + -0.2 487,173 + -0.8 487,174 - +0.8 487,174 - -1.0 487,174 - -0.6 487,174 - +0.4 487,174 - +0.4 487,174 - -0.2 487,178 + +0.8 487,178 + -0.6 487,284 + +1.5 487,284 + -1.7 487,285 - -0.8 487,539 + EX31_RS23610 0.34 -0.0 487,539 + EX31_RS23610 0.34 -0.0 487,540 - EX31_RS23610 0.34 -1.8 487,569 - EX31_RS23610 0.38 -0.6 487,578 + EX31_RS23610 0.39 -0.8 487,778 + EX31_RS23610 0.65 +1.0 487,778 + EX31_RS23610 0.65 +0.2 487,849 + EX31_RS23610 0.74 +0.3 487,849 + EX31_RS23610 0.74 +0.4 487,849 + EX31_RS23610 0.74 +0.4 487,849 + EX31_RS23610 0.74 -1.1 487,849 + EX31_RS23610 0.74 +0.5 487,849 + EX31_RS23610 0.74 +0.4 487,849 + EX31_RS23610 0.74 -0.3 487,849 + EX31_RS23610 0.74 +0.6 487,850 - EX31_RS23610 0.74 +0.5 487,850 - EX31_RS23610 0.74 +0.9 487,850 - EX31_RS23610 0.74 +0.1 487,850 - EX31_RS23610 0.74 -0.8 487,850 - EX31_RS23610 0.74 -0.4 487,850 - EX31_RS23610 0.74 +0.3 487,855 - EX31_RS23610 0.75 -0.3 487,919 + EX31_RS23610 0.83 -0.0 487,919 + EX31_RS23610 0.83 +0.2 487,920 - EX31_RS23610 0.83 +1.2 487,983 + +0.8 487,983 + -0.0 487,989 + -0.7
Or see this region's nucleotide sequence