Experiment: Ammonium chloride (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01805 and EX31_RS01810 are separated by 158 nucleotides EX31_RS01810 and EX31_RS01815 are separated by 229 nucleotides EX31_RS01815 and EX31_RS01820 are separated by 53 nucleotides
EX31_RS01805: EX31_RS01805 - hypothetical protein, at 386,763 to 386,951
_RS01805
EX31_RS01810: EX31_RS01810 - hypothetical protein, at 387,110 to 387,523
_RS01810
EX31_RS01815: EX31_RS01815 - (S)-acetoin forming diacetyl reductase, at 387,753 to 388,532
_RS01815
EX31_RS01820: EX31_RS01820 - helix-turn-helix domain-containing protein, at 388,586 to 389,452
_RS01820
Position (kb) 387
388
389 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 386.757 kb on + strand at 386.757 kb on + strand at 386.855 kb on + strand, within EX31_RS01805 at 386.855 kb on + strand, within EX31_RS01805 at 386.904 kb on - strand, within EX31_RS01805 at 386.937 kb on + strand at 386.937 kb on + strand at 386.938 kb on - strand at 386.938 kb on - strand at 386.938 kb on - strand at 387.046 kb on + strand at 387.046 kb on + strand at 387.047 kb on - strand at 387.111 kb on + strand at 387.111 kb on + strand at 387.113 kb on + strand at 387.113 kb on + strand at 387.114 kb on - strand at 387.114 kb on - strand at 387.177 kb on - strand, within EX31_RS01810 at 387.509 kb on - strand at 387.509 kb on - strand at 387.509 kb on - strand at 387.543 kb on + strand at 387.544 kb on - strand at 387.544 kb on - strand at 387.544 kb on - strand at 387.549 kb on + strand at 387.549 kb on + strand at 387.550 kb on - strand at 387.550 kb on - strand at 387.560 kb on - strand at 387.613 kb on + strand at 387.627 kb on - strand at 387.656 kb on - strand at 387.735 kb on + strand at 387.735 kb on + strand at 387.735 kb on + strand at 387.736 kb on - strand at 387.736 kb on - strand at 387.739 kb on + strand at 387.740 kb on - strand at 387.740 kb on - strand at 387.752 kb on + strand at 387.752 kb on + strand at 387.752 kb on + strand at 387.752 kb on + strand at 387.752 kb on + strand at 387.753 kb on - strand at 387.753 kb on - strand at 387.753 kb on - strand at 387.863 kb on + strand, within EX31_RS01815 at 387.914 kb on + strand, within EX31_RS01815 at 388.013 kb on + strand, within EX31_RS01815 at 388.080 kb on + strand, within EX31_RS01815 at 388.083 kb on - strand, within EX31_RS01815 at 388.083 kb on - strand, within EX31_RS01815 at 388.215 kb on + strand, within EX31_RS01815 at 388.215 kb on + strand, within EX31_RS01815 at 388.286 kb on + strand, within EX31_RS01815 at 388.386 kb on - strand, within EX31_RS01815 at 388.406 kb on + strand, within EX31_RS01815 at 388.482 kb on + strand at 388.562 kb on - strand at 388.608 kb on + strand at 388.609 kb on - strand at 388.609 kb on - strand at 388.609 kb on - strand at 388.612 kb on - strand at 388.612 kb on - strand at 388.651 kb on - strand at 388.676 kb on - strand, within EX31_RS01820 at 388.676 kb on - strand, within EX31_RS01820 at 388.676 kb on - strand, within EX31_RS01820 at 388.711 kb on - strand, within EX31_RS01820 at 388.833 kb on + strand, within EX31_RS01820 at 389.124 kb on + strand, within EX31_RS01820 at 389.124 kb on + strand, within EX31_RS01820 at 389.124 kb on + strand, within EX31_RS01820 at 389.129 kb on - strand, within EX31_RS01820 at 389.193 kb on + strand, within EX31_RS01820 at 389.193 kb on + strand, within EX31_RS01820 at 389.194 kb on - strand, within EX31_RS01820 at 389.194 kb on - strand, within EX31_RS01820 at 389.196 kb on + strand, within EX31_RS01820 at 389.197 kb on - strand, within EX31_RS01820 at 389.221 kb on + strand, within EX31_RS01820 at 389.221 kb on + strand, within EX31_RS01820 at 389.274 kb on + strand, within EX31_RS01820 at 389.274 kb on + strand, within EX31_RS01820 at 389.274 kb on + strand, within EX31_RS01820 at 389.275 kb on - strand, within EX31_RS01820 at 389.275 kb on - strand, within EX31_RS01820 at 389.275 kb on - strand, within EX31_RS01820 at 389.278 kb on + strand, within EX31_RS01820 at 389.322 kb on + strand, within EX31_RS01820 at 389.323 kb on - strand, within EX31_RS01820 at 389.323 kb on - strand, within EX31_RS01820 at 389.323 kb on - strand, within EX31_RS01820 at 389.378 kb on + strand at 389.379 kb on - strand at 389.403 kb on - strand at 389.474 kb on + strand at 389.475 kb on - strand at 389.513 kb on + strand at 389.513 kb on + strand at 389.513 kb on + strand at 389.514 kb on - strand at 389.520 kb on + strand at 389.521 kb on - strand at 389.521 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); RCH2 media; shaking remove 386,757 + -0.2 386,757 + +1.3 386,855 + EX31_RS01805 0.49 +0.5 386,855 + EX31_RS01805 0.49 +1.4 386,904 - EX31_RS01805 0.75 +1.2 386,937 + -0.7 386,937 + +1.2 386,938 - +0.5 386,938 - -0.0 386,938 - -0.1 387,046 + +1.8 387,046 + -0.2 387,047 - -0.5 387,111 + +0.2 387,111 + +0.6 387,113 + +1.8 387,113 + -0.7 387,114 - -0.8 387,114 - +0.2 387,177 - EX31_RS01810 0.16 -1.4 387,509 - +2.8 387,509 - +0.8 387,509 - -0.2 387,543 + -0.9 387,544 - +0.5 387,544 - +1.5 387,544 - -0.5 387,549 + +0.8 387,549 + +0.1 387,550 - -0.1 387,550 - -1.1 387,560 - +1.0 387,613 + +0.5 387,627 - +0.1 387,656 - -0.5 387,735 + -0.1 387,735 + -0.5 387,735 + -0.2 387,736 - +1.1 387,736 - +1.6 387,739 + +2.0 387,740 - -0.0 387,740 - -0.2 387,752 + +1.4 387,752 + +0.0 387,752 + +0.8 387,752 + +0.5 387,752 + +1.0 387,753 - +1.2 387,753 - +0.7 387,753 - -1.2 387,863 + EX31_RS01815 0.14 -1.1 387,914 + EX31_RS01815 0.21 -0.5 388,013 + EX31_RS01815 0.33 +0.2 388,080 + EX31_RS01815 0.42 -0.5 388,083 - EX31_RS01815 0.42 -0.0 388,083 - EX31_RS01815 0.42 -0.1 388,215 + EX31_RS01815 0.59 -1.8 388,215 + EX31_RS01815 0.59 -0.6 388,286 + EX31_RS01815 0.68 +0.0 388,386 - EX31_RS01815 0.81 -0.4 388,406 + EX31_RS01815 0.84 -1.9 388,482 + -1.4 388,562 - +0.8 388,608 + -0.5 388,609 - +0.1 388,609 - +0.6 388,609 - -1.2 388,612 - +1.9 388,612 - -0.4 388,651 - -1.1 388,676 - EX31_RS01820 0.10 -0.1 388,676 - EX31_RS01820 0.10 +0.7 388,676 - EX31_RS01820 0.10 +1.2 388,711 - EX31_RS01820 0.14 -0.5 388,833 + EX31_RS01820 0.28 +2.1 389,124 + EX31_RS01820 0.62 -2.5 389,124 + EX31_RS01820 0.62 +2.3 389,124 + EX31_RS01820 0.62 +0.2 389,129 - EX31_RS01820 0.63 +0.1 389,193 + EX31_RS01820 0.70 +1.8 389,193 + EX31_RS01820 0.70 -0.7 389,194 - EX31_RS01820 0.70 -1.2 389,194 - EX31_RS01820 0.70 +0.8 389,196 + EX31_RS01820 0.70 +0.6 389,197 - EX31_RS01820 0.70 -0.5 389,221 + EX31_RS01820 0.73 +0.2 389,221 + EX31_RS01820 0.73 +0.1 389,274 + EX31_RS01820 0.79 -1.0 389,274 + EX31_RS01820 0.79 -0.8 389,274 + EX31_RS01820 0.79 +0.4 389,275 - EX31_RS01820 0.79 +2.4 389,275 - EX31_RS01820 0.79 +1.8 389,275 - EX31_RS01820 0.79 -1.4 389,278 + EX31_RS01820 0.80 -0.5 389,322 + EX31_RS01820 0.85 +0.1 389,323 - EX31_RS01820 0.85 +2.1 389,323 - EX31_RS01820 0.85 +0.8 389,323 - EX31_RS01820 0.85 +1.8 389,378 + -1.4 389,379 - +0.2 389,403 - -0.7 389,474 + +0.0 389,475 - -0.5 389,513 + +1.8 389,513 + +0.3 389,513 + +0.5 389,514 - +0.7 389,520 + +1.5 389,521 - -1.5 389,521 - +0.2
Or see this region's nucleotide sequence