Experiment: 150_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04620 and AMB_RS04625 are separated by 88 nucleotides AMB_RS04625 and AMB_RS04630 are separated by 105 nucleotides AMB_RS04630 and AMB_RS04635 are separated by 270 nucleotides AMB_RS04635 and AMB_RS04640 are separated by 256 nucleotides AMB_RS04640 and AMB_RS04645 are separated by 265 nucleotides
AMB_RS04620: AMB_RS04620 - 23S ribosomal RNA, at 966,932 to 969,685
_RS04620
AMB_RS04625: AMB_RS04625 - 5S ribosomal RNA, at 969,774 to 969,888
_RS04625
AMB_RS04630: AMB_RS04630 - tRNA-Met, at 969,994 to 970,070
_RS04630
AMB_RS04635: AMB_RS04635 - site-specific integrase, at 970,341 to 971,309
_RS04635
AMB_RS04640: AMB_RS04640 - hypothetical protein, at 971,566 to 971,820
_RS04640
AMB_RS04645: AMB_RS04645 - ATP-dependent helicase, at 972,086 to 974,710
_RS04645
Position (kb) 970
971
972 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 970.030 kb on + strand, within AMB_RS04630 at 970.136 kb on - strand at 970.474 kb on - strand, within AMB_RS04635 at 970.518 kb on + strand, within AMB_RS04635 at 970.518 kb on + strand, within AMB_RS04635 at 970.519 kb on - strand, within AMB_RS04635 at 970.519 kb on - strand, within AMB_RS04635 at 970.519 kb on - strand, within AMB_RS04635 at 970.646 kb on + strand, within AMB_RS04635 at 970.668 kb on + strand, within AMB_RS04635 at 970.668 kb on + strand, within AMB_RS04635 at 970.670 kb on + strand, within AMB_RS04635 at 970.727 kb on + strand, within AMB_RS04635 at 970.727 kb on + strand, within AMB_RS04635 at 970.728 kb on - strand, within AMB_RS04635 at 971.014 kb on + strand, within AMB_RS04635 at 971.241 kb on + strand at 971.242 kb on - strand at 971.252 kb on - strand at 971.339 kb on + strand at 971.351 kb on + strand at 971.352 kb on - strand at 971.385 kb on + strand at 971.418 kb on + strand at 971.419 kb on - strand at 971.456 kb on + strand at 971.456 kb on + strand at 971.457 kb on - strand at 971.565 kb on + strand at 971.565 kb on + strand at 971.565 kb on + strand at 971.565 kb on + strand at 971.566 kb on - strand at 971.566 kb on - strand at 971.708 kb on + strand, within AMB_RS04640 at 971.708 kb on + strand, within AMB_RS04640 at 971.818 kb on + strand at 971.819 kb on - strand at 971.819 kb on - strand at 971.960 kb on - strand at 972.073 kb on + strand at 972.136 kb on + strand at 972.136 kb on + strand at 972.203 kb on - strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.253 kb on + strand at 972.254 kb on - strand at 972.254 kb on - strand at 972.254 kb on - strand at 972.298 kb on + strand at 972.298 kb on + strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand at 972.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_nonmag_2 remove 970,030 + AMB_RS04630 0.47 +1.5 970,136 - -0.5 970,474 - AMB_RS04635 0.14 -4.2 970,518 + AMB_RS04635 0.18 -4.5 970,518 + AMB_RS04635 0.18 -2.8 970,519 - AMB_RS04635 0.18 -1.8 970,519 - AMB_RS04635 0.18 -2.2 970,519 - AMB_RS04635 0.18 -2.2 970,646 + AMB_RS04635 0.31 -0.5 970,668 + AMB_RS04635 0.34 -4.3 970,668 + AMB_RS04635 0.34 -0.9 970,670 + AMB_RS04635 0.34 +1.2 970,727 + AMB_RS04635 0.40 -0.3 970,727 + AMB_RS04635 0.40 +1.0 970,728 - AMB_RS04635 0.40 -0.8 971,014 + AMB_RS04635 0.69 -2.2 971,241 + -2.3 971,242 - -0.1 971,252 - -3.4 971,339 + -0.9 971,351 + -0.4 971,352 - -3.3 971,385 + -1.8 971,418 + +0.4 971,419 - -2.8 971,456 + +0.4 971,456 + -0.5 971,457 - -2.2 971,565 + +1.8 971,565 + +0.6 971,565 + -3.6 971,565 + +1.2 971,566 - -5.1 971,566 - -0.3 971,708 + AMB_RS04640 0.56 -0.3 971,708 + AMB_RS04640 0.56 -0.0 971,818 + -0.5 971,819 - +0.3 971,819 - -0.0 971,960 - -2.4 972,073 + -1.0 972,136 + -2.7 972,136 + +0.0 972,203 - -2.5 972,253 + -1.7 972,253 + -0.8 972,253 + -2.5 972,253 + -3.1 972,253 + +1.0 972,253 + +0.4 972,253 + -0.5 972,254 - +0.2 972,254 - +1.4 972,254 - +0.8 972,298 + +2.1 972,298 + -2.6 972,299 - +0.5 972,299 - -0.5 972,299 - -3.0 972,299 - +0.7 972,299 - -0.4 972,299 - +1.1 972,299 - -5.1
Or see this region's nucleotide sequence