Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3461 and EX28DRAFT_3462 are separated by 164 nucleotides EX28DRAFT_3462 and EX28DRAFT_3463 are separated by 46 nucleotides EX28DRAFT_3463 and EX28DRAFT_3464 are separated by 30 nucleotides EX28DRAFT_3464 and EX28DRAFT_3465 are separated by 106 nucleotides
EX28DRAFT_3461: EX28DRAFT_3461 - ornithine carbamoyltransferase, at 134,882 to 135,886
_3461
EX28DRAFT_3462: EX28DRAFT_3462 - Uncharacterized protein conserved in bacteria, at 136,051 to 136,476
_3462
EX28DRAFT_3463: EX28DRAFT_3463 - Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA, at 136,523 to 137,281
_3463
EX28DRAFT_3464: EX28DRAFT_3464 - hypothetical protein, at 137,312 to 138,034
_3464
EX28DRAFT_3465: EX28DRAFT_3465 - tRNA, at 138,141 to 138,225
_3465
Position (kb) 136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 135.571 kb on - strand, within EX28DRAFT_3461 at 135.661 kb on - strand, within EX28DRAFT_3461 at 135.979 kb on + strand at 136.022 kb on - strand at 136.022 kb on - strand at 136.022 kb on - strand at 136.101 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.144 kb on + strand, within EX28DRAFT_3462 at 136.145 kb on - strand, within EX28DRAFT_3462 at 136.333 kb on - strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.390 kb on + strand, within EX28DRAFT_3462 at 136.391 kb on - strand, within EX28DRAFT_3462 at 136.441 kb on + strand at 136.540 kb on - strand at 136.540 kb on - strand at 136.594 kb on + strand at 136.595 kb on - strand at 136.687 kb on + strand, within EX28DRAFT_3463 at 136.691 kb on + strand, within EX28DRAFT_3463 at 136.742 kb on + strand, within EX28DRAFT_3463 at 136.861 kb on + strand, within EX28DRAFT_3463 at 136.861 kb on + strand, within EX28DRAFT_3463 at 136.862 kb on - strand, within EX28DRAFT_3463 at 136.938 kb on - strand, within EX28DRAFT_3463 at 137.036 kb on + strand, within EX28DRAFT_3463 at 137.141 kb on + strand, within EX28DRAFT_3463 at 137.150 kb on + strand, within EX28DRAFT_3463 at 137.150 kb on + strand, within EX28DRAFT_3463 at 137.151 kb on - strand, within EX28DRAFT_3463 at 137.185 kb on + strand, within EX28DRAFT_3463 at 137.186 kb on - strand, within EX28DRAFT_3463 at 137.186 kb on - strand, within EX28DRAFT_3463 at 137.199 kb on + strand, within EX28DRAFT_3463 at 137.230 kb on + strand at 137.231 kb on - strand at 137.231 kb on - strand at 137.231 kb on - strand at 137.317 kb on + strand at 137.318 kb on - strand at 137.536 kb on + strand, within EX28DRAFT_3464 at 137.615 kb on + strand, within EX28DRAFT_3464 at 137.615 kb on + strand, within EX28DRAFT_3464 at 137.615 kb on + strand, within EX28DRAFT_3464 at 137.616 kb on - strand, within EX28DRAFT_3464 at 137.659 kb on - strand, within EX28DRAFT_3464 at 137.709 kb on + strand, within EX28DRAFT_3464 at 137.854 kb on + strand, within EX28DRAFT_3464 at 137.855 kb on - strand, within EX28DRAFT_3464 at 137.876 kb on + strand, within EX28DRAFT_3464 at 137.944 kb on + strand, within EX28DRAFT_3464 at 138.108 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 135,571 - EX28DRAFT_3461 0.69 -0.2 135,661 - EX28DRAFT_3461 0.78 +0.3 135,979 + +0.2 136,022 - -0.5 136,022 - +1.4 136,022 - +0.4 136,101 + EX28DRAFT_3462 0.12 -0.4 136,144 + EX28DRAFT_3462 0.22 -0.9 136,144 + EX28DRAFT_3462 0.22 -0.4 136,144 + EX28DRAFT_3462 0.22 +0.1 136,144 + EX28DRAFT_3462 0.22 +1.2 136,145 - EX28DRAFT_3462 0.22 +1.3 136,333 - EX28DRAFT_3462 0.66 +1.3 136,390 + EX28DRAFT_3462 0.80 +0.4 136,390 + EX28DRAFT_3462 0.80 +0.8 136,390 + EX28DRAFT_3462 0.80 -0.9 136,390 + EX28DRAFT_3462 0.80 +0.3 136,391 - EX28DRAFT_3462 0.80 +1.0 136,441 + -1.1 136,540 - +0.4 136,540 - -0.9 136,594 + +0.4 136,595 - +0.5 136,687 + EX28DRAFT_3463 0.22 -2.9 136,691 + EX28DRAFT_3463 0.22 -0.1 136,742 + EX28DRAFT_3463 0.29 -0.3 136,861 + EX28DRAFT_3463 0.45 +0.2 136,861 + EX28DRAFT_3463 0.45 -0.4 136,862 - EX28DRAFT_3463 0.45 +0.0 136,938 - EX28DRAFT_3463 0.55 +0.1 137,036 + EX28DRAFT_3463 0.68 -0.2 137,141 + EX28DRAFT_3463 0.81 -3.0 137,150 + EX28DRAFT_3463 0.83 -1.3 137,150 + EX28DRAFT_3463 0.83 -0.3 137,151 - EX28DRAFT_3463 0.83 +0.6 137,185 + EX28DRAFT_3463 0.87 -1.1 137,186 - EX28DRAFT_3463 0.87 -0.3 137,186 - EX28DRAFT_3463 0.87 -0.3 137,199 + EX28DRAFT_3463 0.89 -0.4 137,230 + +0.0 137,231 - -0.2 137,231 - -0.1 137,231 - +0.2 137,317 + -0.3 137,318 - -0.5 137,536 + EX28DRAFT_3464 0.31 -0.0 137,615 + EX28DRAFT_3464 0.42 -1.4 137,615 + EX28DRAFT_3464 0.42 +0.0 137,615 + EX28DRAFT_3464 0.42 -0.1 137,616 - EX28DRAFT_3464 0.42 +0.6 137,659 - EX28DRAFT_3464 0.48 -0.5 137,709 + EX28DRAFT_3464 0.55 -0.7 137,854 + EX28DRAFT_3464 0.75 +0.2 137,855 - EX28DRAFT_3464 0.75 +0.3 137,876 + EX28DRAFT_3464 0.78 -0.1 137,944 + EX28DRAFT_3464 0.87 -0.2 138,108 + -1.3
Or see this region's nucleotide sequence