Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1601 and EX28DRAFT_1602 are separated by 9 nucleotides EX28DRAFT_1602 and EX28DRAFT_1603 are separated by 38 nucleotides EX28DRAFT_1603 and EX28DRAFT_1604 are separated by 64 nucleotides EX28DRAFT_1604 and EX28DRAFT_1605 overlap by 1 nucleotides
EX28DRAFT_1601: EX28DRAFT_1601 - Enterobacterial exodeoxyribonuclease VIII, at 1,615,615 to 1,618,776
_1601
EX28DRAFT_1602: EX28DRAFT_1602 - RecT family, at 1,618,786 to 1,619,871
_1602
EX28DRAFT_1603: EX28DRAFT_1603 - Protein of unknown function (DUF4060), at 1,619,910 to 1,620,152
_1603
EX28DRAFT_1604: EX28DRAFT_1604 - Putative excisionase (DUF1233), at 1,620,217 to 1,620,432
_1604
EX28DRAFT_1605: EX28DRAFT_1605 - Integrase, at 1,620,432 to 1,621,712
_1605
Position (kb)
1618
1619
1620 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1617.843 kb on + strand, within EX28DRAFT_1601 at 1617.844 kb on - strand, within EX28DRAFT_1601 at 1617.844 kb on - strand, within EX28DRAFT_1601 at 1617.904 kb on + strand, within EX28DRAFT_1601 at 1617.904 kb on + strand, within EX28DRAFT_1601 at 1617.905 kb on - strand, within EX28DRAFT_1601 at 1617.905 kb on - strand, within EX28DRAFT_1601 at 1617.907 kb on - strand, within EX28DRAFT_1601 at 1617.907 kb on - strand, within EX28DRAFT_1601 at 1617.926 kb on - strand, within EX28DRAFT_1601 at 1617.933 kb on + strand, within EX28DRAFT_1601 at 1617.933 kb on + strand, within EX28DRAFT_1601 at 1618.146 kb on + strand, within EX28DRAFT_1601 at 1618.147 kb on - strand, within EX28DRAFT_1601 at 1618.272 kb on - strand, within EX28DRAFT_1601 at 1618.334 kb on - strand, within EX28DRAFT_1601 at 1618.501 kb on + strand at 1618.522 kb on + strand at 1618.523 kb on - strand at 1618.583 kb on - strand at 1618.583 kb on - strand at 1618.670 kb on + strand at 1618.671 kb on - strand at 1618.714 kb on + strand at 1618.715 kb on - strand at 1618.785 kb on + strand at 1618.785 kb on + strand at 1618.785 kb on + strand at 1618.785 kb on + strand at 1618.785 kb on + strand at 1618.785 kb on + strand at 1618.786 kb on - strand at 1618.786 kb on - strand at 1618.797 kb on + strand at 1618.830 kb on + strand at 1618.861 kb on + strand at 1618.861 kb on + strand at 1618.912 kb on - strand, within EX28DRAFT_1602 at 1618.912 kb on - strand, within EX28DRAFT_1602 at 1619.102 kb on - strand, within EX28DRAFT_1602 at 1619.102 kb on - strand, within EX28DRAFT_1602 at 1619.235 kb on + strand, within EX28DRAFT_1602 at 1619.336 kb on - strand, within EX28DRAFT_1602 at 1619.336 kb on - strand, within EX28DRAFT_1602 at 1619.472 kb on + strand, within EX28DRAFT_1602 at 1619.590 kb on - strand, within EX28DRAFT_1602 at 1619.878 kb on - strand at 1619.933 kb on + strand at 1619.995 kb on - strand, within EX28DRAFT_1603 at 1620.031 kb on - strand, within EX28DRAFT_1603 at 1620.031 kb on - strand, within EX28DRAFT_1603 at 1620.033 kb on - strand, within EX28DRAFT_1603 at 1620.090 kb on + strand, within EX28DRAFT_1603 at 1620.090 kb on + strand, within EX28DRAFT_1603 at 1620.091 kb on - strand, within EX28DRAFT_1603 at 1620.162 kb on - strand at 1620.171 kb on + strand at 1620.183 kb on + strand at 1620.183 kb on + strand at 1620.184 kb on - strand at 1620.187 kb on + strand at 1620.187 kb on + strand at 1620.187 kb on + strand at 1620.187 kb on + strand at 1620.187 kb on + strand at 1620.187 kb on + strand at 1620.187 kb on + strand at 1620.188 kb on - strand at 1620.188 kb on - strand at 1620.188 kb on - strand at 1620.188 kb on - strand at 1620.188 kb on - strand at 1620.188 kb on - strand at 1620.189 kb on + strand at 1620.232 kb on + strand at 1620.298 kb on - strand, within EX28DRAFT_1604 at 1620.339 kb on + strand, within EX28DRAFT_1604 at 1620.391 kb on + strand, within EX28DRAFT_1604 at 1620.392 kb on - strand, within EX28DRAFT_1604 at 1620.392 kb on - strand, within EX28DRAFT_1604 at 1620.408 kb on + strand, within EX28DRAFT_1604 at 1620.409 kb on - strand, within EX28DRAFT_1604 at 1620.470 kb on + strand at 1620.470 kb on + strand at 1620.482 kb on - strand at 1620.503 kb on + strand at 1620.503 kb on + strand at 1620.535 kb on + strand at 1620.535 kb on + strand at 1620.655 kb on - strand, within EX28DRAFT_1605 at 1620.656 kb on + strand, within EX28DRAFT_1605 at 1620.656 kb on + strand, within EX28DRAFT_1605 at 1620.656 kb on + strand, within EX28DRAFT_1605 at 1620.657 kb on - strand, within EX28DRAFT_1605 at 1620.672 kb on - strand, within EX28DRAFT_1605 at 1620.755 kb on - strand, within EX28DRAFT_1605 at 1620.760 kb on + strand, within EX28DRAFT_1605 at 1620.778 kb on + strand, within EX28DRAFT_1605 at 1620.844 kb on + strand, within EX28DRAFT_1605 at 1620.861 kb on - strand, within EX28DRAFT_1605 at 1620.864 kb on + strand, within EX28DRAFT_1605 at 1620.864 kb on + strand, within EX28DRAFT_1605 at 1620.864 kb on + strand, within EX28DRAFT_1605 at 1620.864 kb on + strand, within EX28DRAFT_1605 at 1620.865 kb on - strand, within EX28DRAFT_1605
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,617,843 + EX28DRAFT_1601 0.70 +1.2 1,617,844 - EX28DRAFT_1601 0.70 +0.3 1,617,844 - EX28DRAFT_1601 0.70 -0.1 1,617,904 + EX28DRAFT_1601 0.72 -0.4 1,617,904 + EX28DRAFT_1601 0.72 +0.2 1,617,905 - EX28DRAFT_1601 0.72 +1.8 1,617,905 - EX28DRAFT_1601 0.72 -0.0 1,617,907 - EX28DRAFT_1601 0.72 +2.4 1,617,907 - EX28DRAFT_1601 0.72 -0.5 1,617,926 - EX28DRAFT_1601 0.73 +0.3 1,617,933 + EX28DRAFT_1601 0.73 -0.1 1,617,933 + EX28DRAFT_1601 0.73 -0.3 1,618,146 + EX28DRAFT_1601 0.80 +0.6 1,618,147 - EX28DRAFT_1601 0.80 -0.9 1,618,272 - EX28DRAFT_1601 0.84 +0.4 1,618,334 - EX28DRAFT_1601 0.86 +0.3 1,618,501 + -0.4 1,618,522 + -0.7 1,618,523 - -0.4 1,618,583 - +0.6 1,618,583 - +0.1 1,618,670 + +0.1 1,618,671 - -0.5 1,618,714 + -0.2 1,618,715 - +0.7 1,618,785 + +0.4 1,618,785 + +0.2 1,618,785 + +0.7 1,618,785 + +0.2 1,618,785 + +0.7 1,618,785 + +1.2 1,618,786 - -0.1 1,618,786 - -0.2 1,618,797 + +1.5 1,618,830 + -0.1 1,618,861 + +1.2 1,618,861 + -0.1 1,618,912 - EX28DRAFT_1602 0.12 +0.2 1,618,912 - EX28DRAFT_1602 0.12 -0.6 1,619,102 - EX28DRAFT_1602 0.29 +0.4 1,619,102 - EX28DRAFT_1602 0.29 -2.2 1,619,235 + EX28DRAFT_1602 0.41 -1.4 1,619,336 - EX28DRAFT_1602 0.51 -0.0 1,619,336 - EX28DRAFT_1602 0.51 +0.1 1,619,472 + EX28DRAFT_1602 0.63 -0.6 1,619,590 - EX28DRAFT_1602 0.74 -0.7 1,619,878 - +0.4 1,619,933 + -0.7 1,619,995 - EX28DRAFT_1603 0.35 -0.5 1,620,031 - EX28DRAFT_1603 0.50 +0.1 1,620,031 - EX28DRAFT_1603 0.50 -0.2 1,620,033 - EX28DRAFT_1603 0.51 -1.0 1,620,090 + EX28DRAFT_1603 0.74 -0.0 1,620,090 + EX28DRAFT_1603 0.74 +0.1 1,620,091 - EX28DRAFT_1603 0.74 -0.0 1,620,162 - +1.3 1,620,171 + -0.9 1,620,183 + -0.8 1,620,183 + +1.5 1,620,184 - +0.4 1,620,187 + +0.4 1,620,187 + +1.4 1,620,187 + +2.0 1,620,187 + +1.3 1,620,187 + -0.5 1,620,187 + +0.4 1,620,187 + +0.2 1,620,188 - +2.1 1,620,188 - +0.0 1,620,188 - -0.4 1,620,188 - -1.1 1,620,188 - +0.1 1,620,188 - +0.4 1,620,189 + +0.9 1,620,232 + +0.5 1,620,298 - EX28DRAFT_1604 0.38 +0.3 1,620,339 + EX28DRAFT_1604 0.56 -0.2 1,620,391 + EX28DRAFT_1604 0.81 +0.5 1,620,392 - EX28DRAFT_1604 0.81 -2.3 1,620,392 - EX28DRAFT_1604 0.81 -0.3 1,620,408 + EX28DRAFT_1604 0.88 -0.2 1,620,409 - EX28DRAFT_1604 0.89 -0.1 1,620,470 + -0.3 1,620,470 + -0.3 1,620,482 - -0.5 1,620,503 + +0.4 1,620,503 + -0.3 1,620,535 + -0.2 1,620,535 + -0.4 1,620,655 - EX28DRAFT_1605 0.17 +0.8 1,620,656 + EX28DRAFT_1605 0.17 -0.1 1,620,656 + EX28DRAFT_1605 0.17 +1.3 1,620,656 + EX28DRAFT_1605 0.17 +0.1 1,620,657 - EX28DRAFT_1605 0.18 +0.3 1,620,672 - EX28DRAFT_1605 0.19 -0.3 1,620,755 - EX28DRAFT_1605 0.25 +0.0 1,620,760 + EX28DRAFT_1605 0.26 -0.2 1,620,778 + EX28DRAFT_1605 0.27 +0.3 1,620,844 + EX28DRAFT_1605 0.32 -0.3 1,620,861 - EX28DRAFT_1605 0.33 -0.0 1,620,864 + EX28DRAFT_1605 0.34 +0.6 1,620,864 + EX28DRAFT_1605 0.34 -0.4 1,620,864 + EX28DRAFT_1605 0.34 -0.2 1,620,864 + EX28DRAFT_1605 0.34 -0.2 1,620,865 - EX28DRAFT_1605 0.34 -0.8
Or see this region's nucleotide sequence