Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3784 and EX28DRAFT_3785 are separated by 106 nucleotides EX28DRAFT_3785 and EX28DRAFT_3786 are separated by 40 nucleotides EX28DRAFT_3786 and EX28DRAFT_3787 overlap by 8 nucleotides
EX28DRAFT_3784: EX28DRAFT_3784 - hypothetical protein, at 466,037 to 466,441
_3784
EX28DRAFT_3785: EX28DRAFT_3785 - lipoprotein, YaeC family, at 466,548 to 467,363
_3785
EX28DRAFT_3786: EX28DRAFT_3786 - ABC-type metal ion transport system, permease component, at 467,404 to 468,057
_3786
EX28DRAFT_3787: EX28DRAFT_3787 - D-methionine ABC transporter, ATP-binding protein, at 468,050 to 469,081
_3787
Position (kb) 467
468
469 Strain fitness (log2 ratio) -2
-1
0
1
2 at 466.441 kb on + strand at 466.444 kb on + strand at 466.444 kb on + strand at 466.445 kb on - strand at 466.445 kb on - strand at 466.445 kb on - strand at 466.476 kb on - strand at 466.514 kb on - strand at 466.530 kb on + strand at 466.533 kb on + strand at 466.533 kb on + strand at 466.533 kb on + strand at 466.534 kb on - strand at 466.534 kb on - strand at 466.534 kb on - strand at 466.559 kb on + strand at 466.615 kb on + strand at 466.616 kb on - strand at 466.697 kb on + strand, within EX28DRAFT_3785 at 466.750 kb on + strand, within EX28DRAFT_3785 at 466.751 kb on - strand, within EX28DRAFT_3785 at 466.751 kb on - strand, within EX28DRAFT_3785 at 467.061 kb on - strand, within EX28DRAFT_3785 at 467.092 kb on + strand, within EX28DRAFT_3785 at 467.092 kb on + strand, within EX28DRAFT_3785 at 467.100 kb on - strand, within EX28DRAFT_3785 at 467.158 kb on + strand, within EX28DRAFT_3785 at 467.158 kb on + strand, within EX28DRAFT_3785 at 467.194 kb on + strand, within EX28DRAFT_3785 at 467.194 kb on + strand, within EX28DRAFT_3785 at 467.195 kb on - strand, within EX28DRAFT_3785 at 467.264 kb on + strand, within EX28DRAFT_3785 at 467.331 kb on + strand at 467.332 kb on - strand at 467.350 kb on - strand at 467.355 kb on - strand at 467.374 kb on + strand at 467.399 kb on + strand at 467.405 kb on + strand at 467.456 kb on + strand at 467.456 kb on + strand at 467.505 kb on + strand, within EX28DRAFT_3786 at 467.505 kb on + strand, within EX28DRAFT_3786 at 467.506 kb on - strand, within EX28DRAFT_3786 at 467.506 kb on - strand, within EX28DRAFT_3786 at 467.507 kb on + strand, within EX28DRAFT_3786 at 467.515 kb on - strand, within EX28DRAFT_3786 at 467.517 kb on - strand, within EX28DRAFT_3786 at 467.764 kb on + strand, within EX28DRAFT_3786 at 467.765 kb on - strand, within EX28DRAFT_3786 at 467.863 kb on - strand, within EX28DRAFT_3786 at 467.863 kb on - strand, within EX28DRAFT_3786 at 467.943 kb on - strand, within EX28DRAFT_3786 at 467.943 kb on - strand, within EX28DRAFT_3786 at 467.943 kb on - strand, within EX28DRAFT_3786 at 467.951 kb on - strand, within EX28DRAFT_3786 at 468.057 kb on + strand at 468.058 kb on - strand at 468.077 kb on + strand at 468.078 kb on - strand at 468.078 kb on - strand at 468.175 kb on - strand, within EX28DRAFT_3787 at 468.407 kb on + strand, within EX28DRAFT_3787 at 468.408 kb on - strand, within EX28DRAFT_3787 at 468.490 kb on + strand, within EX28DRAFT_3787 at 468.490 kb on + strand, within EX28DRAFT_3787 at 468.491 kb on - strand, within EX28DRAFT_3787 at 468.491 kb on - strand, within EX28DRAFT_3787 at 468.491 kb on - strand, within EX28DRAFT_3787 at 468.491 kb on - strand, within EX28DRAFT_3787 at 468.493 kb on + strand, within EX28DRAFT_3787 at 468.493 kb on + strand, within EX28DRAFT_3787 at 468.494 kb on - strand, within EX28DRAFT_3787 at 468.599 kb on + strand, within EX28DRAFT_3787 at 468.676 kb on + strand, within EX28DRAFT_3787 at 468.896 kb on + strand, within EX28DRAFT_3787 at 468.952 kb on + strand, within EX28DRAFT_3787 at 468.953 kb on - strand, within EX28DRAFT_3787
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 466,441 + -0.1 466,444 + +0.0 466,444 + -0.2 466,445 - -0.6 466,445 - +0.4 466,445 - -0.1 466,476 - -0.2 466,514 - -1.2 466,530 + -1.1 466,533 + -0.3 466,533 + -0.4 466,533 + -0.1 466,534 - -1.1 466,534 - -2.5 466,534 - -0.4 466,559 + -0.5 466,615 + +0.1 466,616 - +0.6 466,697 + EX28DRAFT_3785 0.18 +0.9 466,750 + EX28DRAFT_3785 0.25 -0.6 466,751 - EX28DRAFT_3785 0.25 +0.1 466,751 - EX28DRAFT_3785 0.25 +0.2 467,061 - EX28DRAFT_3785 0.63 -0.2 467,092 + EX28DRAFT_3785 0.67 -0.6 467,092 + EX28DRAFT_3785 0.67 -0.1 467,100 - EX28DRAFT_3785 0.68 -0.1 467,158 + EX28DRAFT_3785 0.75 -0.4 467,158 + EX28DRAFT_3785 0.75 -2.1 467,194 + EX28DRAFT_3785 0.79 +1.9 467,194 + EX28DRAFT_3785 0.79 +0.9 467,195 - EX28DRAFT_3785 0.79 -0.2 467,264 + EX28DRAFT_3785 0.88 +1.7 467,331 + +0.1 467,332 - -0.4 467,350 - -0.9 467,355 - +0.4 467,374 + -0.1 467,399 + -0.2 467,405 + +0.0 467,456 + -0.5 467,456 + -1.5 467,505 + EX28DRAFT_3786 0.15 +0.1 467,505 + EX28DRAFT_3786 0.15 +0.1 467,506 - EX28DRAFT_3786 0.16 -0.5 467,506 - EX28DRAFT_3786 0.16 -0.1 467,507 + EX28DRAFT_3786 0.16 +0.5 467,515 - EX28DRAFT_3786 0.17 -0.0 467,517 - EX28DRAFT_3786 0.17 +1.1 467,764 + EX28DRAFT_3786 0.55 -0.3 467,765 - EX28DRAFT_3786 0.55 +0.9 467,863 - EX28DRAFT_3786 0.70 +0.0 467,863 - EX28DRAFT_3786 0.70 -0.5 467,943 - EX28DRAFT_3786 0.82 +0.2 467,943 - EX28DRAFT_3786 0.82 +1.0 467,943 - EX28DRAFT_3786 0.82 -0.1 467,951 - EX28DRAFT_3786 0.84 +0.1 468,057 + +0.0 468,058 - -1.1 468,077 + -0.1 468,078 - -0.7 468,078 - -0.0 468,175 - EX28DRAFT_3787 0.12 +0.2 468,407 + EX28DRAFT_3787 0.35 -0.6 468,408 - EX28DRAFT_3787 0.35 +1.8 468,490 + EX28DRAFT_3787 0.43 +0.6 468,490 + EX28DRAFT_3787 0.43 +0.5 468,491 - EX28DRAFT_3787 0.43 +0.2 468,491 - EX28DRAFT_3787 0.43 +0.6 468,491 - EX28DRAFT_3787 0.43 +0.3 468,491 - EX28DRAFT_3787 0.43 +1.1 468,493 + EX28DRAFT_3787 0.43 +0.3 468,493 + EX28DRAFT_3787 0.43 +0.2 468,494 - EX28DRAFT_3787 0.43 +0.9 468,599 + EX28DRAFT_3787 0.53 +1.1 468,676 + EX28DRAFT_3787 0.61 -1.8 468,896 + EX28DRAFT_3787 0.82 +0.9 468,952 + EX28DRAFT_3787 0.87 -0.1 468,953 - EX28DRAFT_3787 0.88 +0.4
Or see this region's nucleotide sequence