Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2014 and EX28DRAFT_2015 are separated by 3 nucleotides EX28DRAFT_2015 and EX28DRAFT_2016 are separated by 124 nucleotides EX28DRAFT_2016 and EX28DRAFT_2017 overlap by 24 nucleotides
EX28DRAFT_2014: EX28DRAFT_2014 - bacterial condensin subunit MukF, at 2,024,491 to 2,025,813
_2014
EX28DRAFT_2015: EX28DRAFT_2015 - 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase, at 2,025,817 to 2,026,596
_2015
EX28DRAFT_2016: EX28DRAFT_2016 - Uncharacterized conserved protein, at 2,026,721 to 2,027,500
_2016
EX28DRAFT_2017: EX28DRAFT_2017 - Phosphotransferase enzyme family, at 2,027,477 to 2,028,370
_2017
Position (kb)
2025
2026
2027 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2025.856 kb on - strand at 2025.856 kb on - strand at 2025.856 kb on - strand at 2025.858 kb on - strand at 2025.905 kb on - strand, within EX28DRAFT_2015 at 2025.957 kb on - strand, within EX28DRAFT_2015 at 2026.098 kb on - strand, within EX28DRAFT_2015 at 2026.149 kb on - strand, within EX28DRAFT_2015 at 2026.202 kb on - strand, within EX28DRAFT_2015 at 2026.305 kb on + strand, within EX28DRAFT_2015 at 2026.306 kb on - strand, within EX28DRAFT_2015 at 2026.670 kb on + strand at 2026.670 kb on + strand at 2026.670 kb on + strand at 2026.671 kb on - strand at 2026.674 kb on - strand at 2026.674 kb on - strand at 2026.683 kb on - strand at 2026.683 kb on - strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.694 kb on - strand at 2026.694 kb on - strand at 2026.695 kb on + strand at 2026.695 kb on + strand at 2026.696 kb on - strand at 2026.696 kb on - strand at 2026.736 kb on - strand at 2026.742 kb on + strand at 2026.743 kb on - strand at 2026.743 kb on - strand at 2026.846 kb on + strand, within EX28DRAFT_2016 at 2026.849 kb on + strand, within EX28DRAFT_2016 at 2026.867 kb on + strand, within EX28DRAFT_2016 at 2026.909 kb on - strand, within EX28DRAFT_2016 at 2026.961 kb on + strand, within EX28DRAFT_2016 at 2026.962 kb on - strand, within EX28DRAFT_2016 at 2026.985 kb on + strand, within EX28DRAFT_2016 at 2026.985 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.988 kb on - strand, within EX28DRAFT_2016 at 2027.212 kb on + strand, within EX28DRAFT_2016 at 2027.419 kb on + strand, within EX28DRAFT_2016 at 2027.578 kb on - strand, within EX28DRAFT_2017
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,025,856 - +1.7 2,025,856 - -0.3 2,025,856 - -1.8 2,025,858 - -2.0 2,025,905 - EX28DRAFT_2015 0.11 -1.0 2,025,957 - EX28DRAFT_2015 0.18 +0.8 2,026,098 - EX28DRAFT_2015 0.36 +2.4 2,026,149 - EX28DRAFT_2015 0.43 -0.2 2,026,202 - EX28DRAFT_2015 0.49 +0.6 2,026,305 + EX28DRAFT_2015 0.63 +0.9 2,026,306 - EX28DRAFT_2015 0.63 +0.1 2,026,670 + -0.6 2,026,670 + +0.3 2,026,670 + -0.2 2,026,671 - +0.1 2,026,674 - -0.2 2,026,674 - +0.4 2,026,683 - -1.0 2,026,683 - +0.0 2,026,693 + +1.8 2,026,693 + +0.2 2,026,693 + +0.0 2,026,693 + -1.9 2,026,694 - +0.1 2,026,694 - +0.2 2,026,695 + +0.1 2,026,695 + +0.8 2,026,696 - -0.6 2,026,696 - -0.4 2,026,736 - +0.1 2,026,742 + -0.2 2,026,743 - +0.1 2,026,743 - +0.6 2,026,846 + EX28DRAFT_2016 0.16 +0.4 2,026,849 + EX28DRAFT_2016 0.16 +0.4 2,026,867 + EX28DRAFT_2016 0.19 +0.9 2,026,909 - EX28DRAFT_2016 0.24 -0.6 2,026,961 + EX28DRAFT_2016 0.31 -0.1 2,026,962 - EX28DRAFT_2016 0.31 +0.2 2,026,985 + EX28DRAFT_2016 0.34 +0.5 2,026,985 + EX28DRAFT_2016 0.34 -0.0 2,026,987 + EX28DRAFT_2016 0.34 -0.0 2,026,987 + EX28DRAFT_2016 0.34 +0.3 2,026,987 + EX28DRAFT_2016 0.34 -0.1 2,026,987 + EX28DRAFT_2016 0.34 +0.7 2,026,988 - EX28DRAFT_2016 0.34 -1.7 2,027,212 + EX28DRAFT_2016 0.63 -0.2 2,027,419 + EX28DRAFT_2016 0.89 -0.2 2,027,578 - EX28DRAFT_2017 0.11 +0.3
Or see this region's nucleotide sequence