Experiment: L-Serine (N); RCH2 media; shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06975 and EX31_RS06980 are separated by 162 nucleotides EX31_RS06980 and EX31_RS06985 overlap by 14 nucleotides EX31_RS06985 and EX31_RS06990 are separated by 50 nucleotides
EX31_RS06975: EX31_RS06975 - YdgA family protein, at 184,037 to 185,578
_RS06975
EX31_RS06980: EX31_RS06980 - iron ABC transporter ATP-binding protein FetA, at 185,741 to 186,412
_RS06980
EX31_RS06985: EX31_RS06985 - iron export ABC transporter permease subunit FetB, at 186,399 to 187,172
_RS06985
EX31_RS06990: EX31_RS06990 - GntR family transcriptional regulator, at 187,223 to 187,918
_RS06990
Position (kb) 186
187
188 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 185.404 kb on + strand, within EX31_RS06975 at 185.404 kb on + strand, within EX31_RS06975 at 185.408 kb on - strand, within EX31_RS06975 at 185.576 kb on + strand at 185.711 kb on - strand at 185.785 kb on + strand at 185.846 kb on - strand, within EX31_RS06980 at 185.882 kb on - strand, within EX31_RS06980 at 185.882 kb on - strand, within EX31_RS06980 at 185.896 kb on + strand, within EX31_RS06980 at 185.896 kb on + strand, within EX31_RS06980 at 185.934 kb on - strand, within EX31_RS06980 at 185.994 kb on - strand, within EX31_RS06980 at 186.015 kb on + strand, within EX31_RS06980 at 186.015 kb on + strand, within EX31_RS06980 at 186.256 kb on + strand, within EX31_RS06980 at 186.410 kb on + strand at 186.411 kb on - strand at 186.413 kb on + strand at 186.413 kb on + strand at 186.413 kb on + strand at 186.413 kb on + strand at 186.532 kb on + strand, within EX31_RS06985 at 186.657 kb on - strand, within EX31_RS06985 at 186.657 kb on - strand, within EX31_RS06985 at 186.675 kb on - strand, within EX31_RS06985 at 186.822 kb on + strand, within EX31_RS06985 at 186.834 kb on - strand, within EX31_RS06985 at 186.955 kb on + strand, within EX31_RS06985 at 187.047 kb on + strand, within EX31_RS06985 at 187.101 kb on - strand at 187.101 kb on - strand at 187.111 kb on - strand at 187.131 kb on + strand at 187.132 kb on - strand at 187.173 kb on + strand at 187.174 kb on - strand at 187.177 kb on + strand at 187.225 kb on - strand at 187.227 kb on - strand at 187.337 kb on - strand, within EX31_RS06990 at 187.583 kb on - strand, within EX31_RS06990 at 187.583 kb on - strand, within EX31_RS06990 at 187.583 kb on - strand, within EX31_RS06990 at 187.585 kb on + strand, within EX31_RS06990 at 187.586 kb on - strand, within EX31_RS06990 at 187.715 kb on - strand, within EX31_RS06990 at 187.715 kb on - strand, within EX31_RS06990 at 187.755 kb on - strand, within EX31_RS06990 at 187.913 kb on - strand at 187.961 kb on - strand at 187.990 kb on - strand at 187.990 kb on - strand at 188.039 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; shaking remove 185,404 + EX31_RS06975 0.89 +0.4 185,404 + EX31_RS06975 0.89 +0.1 185,408 - EX31_RS06975 0.89 +0.4 185,576 + -0.6 185,711 - +1.4 185,785 + -1.6 185,846 - EX31_RS06980 0.16 -0.6 185,882 - EX31_RS06980 0.21 -0.1 185,882 - EX31_RS06980 0.21 -0.2 185,896 + EX31_RS06980 0.23 -0.9 185,896 + EX31_RS06980 0.23 -1.2 185,934 - EX31_RS06980 0.29 +0.8 185,994 - EX31_RS06980 0.38 -0.9 186,015 + EX31_RS06980 0.41 +2.1 186,015 + EX31_RS06980 0.41 +0.3 186,256 + EX31_RS06980 0.77 -0.2 186,410 + +0.3 186,411 - -1.2 186,413 + -0.2 186,413 + -0.6 186,413 + -0.3 186,413 + +1.2 186,532 + EX31_RS06985 0.17 -0.2 186,657 - EX31_RS06985 0.33 +0.4 186,657 - EX31_RS06985 0.33 +0.4 186,675 - EX31_RS06985 0.36 -0.9 186,822 + EX31_RS06985 0.55 -0.2 186,834 - EX31_RS06985 0.56 -0.4 186,955 + EX31_RS06985 0.72 -0.3 187,047 + EX31_RS06985 0.84 -0.7 187,101 - +0.2 187,101 - +0.4 187,111 - -0.2 187,131 + -1.6 187,132 - +2.4 187,173 + -0.6 187,174 - +1.7 187,177 + -0.2 187,225 - +0.1 187,227 - -0.2 187,337 - EX31_RS06990 0.16 -1.4 187,583 - EX31_RS06990 0.52 +0.8 187,583 - EX31_RS06990 0.52 +0.8 187,583 - EX31_RS06990 0.52 -0.5 187,585 + EX31_RS06990 0.52 +0.3 187,586 - EX31_RS06990 0.52 +0.4 187,715 - EX31_RS06990 0.71 +0.4 187,715 - EX31_RS06990 0.71 +1.2 187,755 - EX31_RS06990 0.76 -0.7 187,913 - -0.6 187,961 - +3.8 187,990 - -0.9 187,990 - -0.5 188,039 + +0.8
Or see this region's nucleotide sequence