Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2501 and EX28DRAFT_2502 are separated by 101 nucleotides EX28DRAFT_2502 and EX28DRAFT_2503 overlap by 4 nucleotides EX28DRAFT_2503 and EX28DRAFT_2504 are separated by 2 nucleotides EX28DRAFT_2504 and EX28DRAFT_2505 are separated by 218 nucleotides
EX28DRAFT_2501: EX28DRAFT_2501 - Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs, at 2,532,535 to 2,533,995
_2501
EX28DRAFT_2502: EX28DRAFT_2502 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 2,534,097 to 2,534,807
_2502
EX28DRAFT_2503: EX28DRAFT_2503 - ribosomal protein L36, bacterial type, at 2,534,804 to 2,534,944
_2503
EX28DRAFT_2504: EX28DRAFT_2504 - ribosomal protein L31, at 2,534,947 to 2,535,207
_2504
EX28DRAFT_2505: EX28DRAFT_2505 - Predicted signal transduction protein containing sensor and EAL domains, at 2,535,426 to 2,536,991
_2505
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2533.987 kb on + strand at 2533.988 kb on - strand at 2534.248 kb on - strand, within EX28DRAFT_2502 at 2534.509 kb on - strand, within EX28DRAFT_2502 at 2534.523 kb on + strand, within EX28DRAFT_2502 at 2534.524 kb on - strand, within EX28DRAFT_2502 at 2534.650 kb on + strand, within EX28DRAFT_2502 at 2534.849 kb on - strand, within EX28DRAFT_2503 at 2534.866 kb on - strand, within EX28DRAFT_2503 at 2534.889 kb on - strand, within EX28DRAFT_2503 at 2534.932 kb on + strand at 2534.932 kb on + strand at 2534.933 kb on - strand at 2534.937 kb on - strand at 2535.031 kb on - strand, within EX28DRAFT_2504 at 2535.077 kb on + strand, within EX28DRAFT_2504 at 2535.077 kb on + strand, within EX28DRAFT_2504 at 2535.078 kb on - strand, within EX28DRAFT_2504 at 2535.078 kb on - strand, within EX28DRAFT_2504 at 2535.121 kb on - strand, within EX28DRAFT_2504 at 2535.144 kb on - strand, within EX28DRAFT_2504 at 2535.144 kb on - strand, within EX28DRAFT_2504 at 2535.211 kb on - strand at 2535.230 kb on - strand at 2535.237 kb on - strand at 2535.237 kb on - strand at 2535.245 kb on - strand at 2535.248 kb on - strand at 2535.340 kb on - strand at 2535.343 kb on + strand at 2535.343 kb on + strand at 2535.343 kb on + strand at 2535.344 kb on - strand at 2535.405 kb on + strand at 2535.463 kb on + strand at 2535.464 kb on - strand at 2535.464 kb on - strand at 2535.468 kb on - strand at 2535.468 kb on - strand at 2535.552 kb on - strand at 2535.555 kb on + strand at 2535.555 kb on + strand at 2535.556 kb on - strand at 2535.556 kb on - strand at 2535.556 kb on - strand at 2535.647 kb on + strand, within EX28DRAFT_2505 at 2535.696 kb on + strand, within EX28DRAFT_2505 at 2535.696 kb on + strand, within EX28DRAFT_2505 at 2535.714 kb on + strand, within EX28DRAFT_2505 at 2535.714 kb on + strand, within EX28DRAFT_2505 at 2535.715 kb on - strand, within EX28DRAFT_2505 at 2535.715 kb on - strand, within EX28DRAFT_2505 at 2535.715 kb on - strand, within EX28DRAFT_2505 at 2535.716 kb on + strand, within EX28DRAFT_2505 at 2535.791 kb on + strand, within EX28DRAFT_2505 at 2535.795 kb on - strand, within EX28DRAFT_2505 at 2535.808 kb on - strand, within EX28DRAFT_2505 at 2535.852 kb on + strand, within EX28DRAFT_2505 at 2535.879 kb on + strand, within EX28DRAFT_2505 at 2535.880 kb on - strand, within EX28DRAFT_2505 at 2535.976 kb on + strand, within EX28DRAFT_2505 at 2536.050 kb on + strand, within EX28DRAFT_2505 at 2536.065 kb on + strand, within EX28DRAFT_2505 at 2536.066 kb on - strand, within EX28DRAFT_2505 at 2536.066 kb on - strand, within EX28DRAFT_2505 at 2536.082 kb on - strand, within EX28DRAFT_2505 at 2536.097 kb on + strand, within EX28DRAFT_2505 at 2536.183 kb on + strand, within EX28DRAFT_2505
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,533,987 + +0.2 2,533,988 - +0.1 2,534,248 - EX28DRAFT_2502 0.21 +0.5 2,534,509 - EX28DRAFT_2502 0.58 +0.2 2,534,523 + EX28DRAFT_2502 0.60 +1.0 2,534,524 - EX28DRAFT_2502 0.60 +0.2 2,534,650 + EX28DRAFT_2502 0.78 -1.9 2,534,849 - EX28DRAFT_2503 0.32 -0.1 2,534,866 - EX28DRAFT_2503 0.44 +0.2 2,534,889 - EX28DRAFT_2503 0.60 +0.1 2,534,932 + -0.0 2,534,932 + -0.4 2,534,933 - -0.3 2,534,937 - +0.6 2,535,031 - EX28DRAFT_2504 0.32 +0.1 2,535,077 + EX28DRAFT_2504 0.50 +0.5 2,535,077 + EX28DRAFT_2504 0.50 +0.2 2,535,078 - EX28DRAFT_2504 0.50 -2.2 2,535,078 - EX28DRAFT_2504 0.50 +0.3 2,535,121 - EX28DRAFT_2504 0.67 +0.1 2,535,144 - EX28DRAFT_2504 0.75 +1.6 2,535,144 - EX28DRAFT_2504 0.75 -0.0 2,535,211 - -0.5 2,535,230 - -0.1 2,535,237 - -0.0 2,535,237 - -0.4 2,535,245 - +0.9 2,535,248 - -0.2 2,535,340 - +0.2 2,535,343 + -1.0 2,535,343 + -0.7 2,535,343 + -1.2 2,535,344 - -0.8 2,535,405 + -0.3 2,535,463 + -0.3 2,535,464 - +1.0 2,535,464 - -1.2 2,535,468 - +0.2 2,535,468 - -0.2 2,535,552 - -1.1 2,535,555 + +3.6 2,535,555 + -0.0 2,535,556 - -0.1 2,535,556 - -0.8 2,535,556 - -0.1 2,535,647 + EX28DRAFT_2505 0.14 -0.2 2,535,696 + EX28DRAFT_2505 0.17 +0.1 2,535,696 + EX28DRAFT_2505 0.17 +0.8 2,535,714 + EX28DRAFT_2505 0.18 -1.0 2,535,714 + EX28DRAFT_2505 0.18 -0.2 2,535,715 - EX28DRAFT_2505 0.18 +1.0 2,535,715 - EX28DRAFT_2505 0.18 -0.3 2,535,715 - EX28DRAFT_2505 0.18 -0.2 2,535,716 + EX28DRAFT_2505 0.19 +0.6 2,535,791 + EX28DRAFT_2505 0.23 +0.6 2,535,795 - EX28DRAFT_2505 0.24 +0.3 2,535,808 - EX28DRAFT_2505 0.24 -0.1 2,535,852 + EX28DRAFT_2505 0.27 -0.5 2,535,879 + EX28DRAFT_2505 0.29 +0.9 2,535,880 - EX28DRAFT_2505 0.29 -0.0 2,535,976 + EX28DRAFT_2505 0.35 +0.0 2,536,050 + EX28DRAFT_2505 0.40 +0.1 2,536,065 + EX28DRAFT_2505 0.41 -0.3 2,536,066 - EX28DRAFT_2505 0.41 -1.8 2,536,066 - EX28DRAFT_2505 0.41 -0.4 2,536,082 - EX28DRAFT_2505 0.42 +0.0 2,536,097 + EX28DRAFT_2505 0.43 -0.4 2,536,183 + EX28DRAFT_2505 0.48 +0.3
Or see this region's nucleotide sequence